公开(公告)号：EP0959901A4

公开(公告)日：2002-11-04

申请号：EP97936185

申请日：1997-07-31

申请人： UNIV MARYLAND BIOTECH INST

发明人： VAKHARIA VIKRAM N ,  MUNDT EGBERT

IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K39/12 ,  A61P31/12 ,  C07K14/08 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N7/00 ,  C12P21/02 ,  A61K39/38 ,  C12N7/01 ,  C12N7/04 ,  C12N7/06 ,  C12N7/08 ,  C12N15/00 ,  C12P21/04

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K2039/525 ,  C12N2720/10051 ,  Y10S424/816 ,  Y10S424/826

摘要： A system for the generation of live Birnavirus such as infectious bursal disease virus (IBDV), a segmented double-stranded (ds)RNA virus of the Birnavirdae family, using synthetic transcripts derived from cloned DNA has been developed. Independent full-length cDNA clones were constructed which contained the entire coding and non-coding regions of RNA segments A and B of IBDV, respectively. Synthetic RNAs of both segments were produced by in vitro transcription of linearized plasmids with T7 RNA polymerase. Transfection of Vero cells with combined plus-sense transcripts of both segments generated infectious virus as early as 36 hours post-transfection. The development of a reverse genetics system for dsRNA viruses will greatly facilitate studies of the regulation of viral gene expression pathogenesis, and design of a new generation of live and inactivated vaccines.