公开(公告)号：EP1930026A1

公开(公告)日：2008-06-11

申请号：EP07012332.8

申请日：1997-07-31

申请人： UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE

发明人： Mundt, Egbert ,  Vakharia, Vikram N.

IPC分类号： A61K39/12 ,  C12N7/02 ,  C12N15/63 ,  C12N15/86

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K2039/525 ,  C12N2720/10051 ,  Y10S424/816 ,  Y10S424/826

摘要： A system for the generation of live Birnavirus such as infectious bursal disease virus (IBDV), a segmented double-stranded (ds)RNA virus of the Birnavirdae family, using synthetic transcripts derived from cloned DNA has been developed. Independent full-length cDMA clones were constructed which contained the entire coding and noncoding regions of RNA segments A and B of IBDV, respectively. Synthetic RNAs of both segments were produced by in viro transcription of linearized plasmids with T7 RNA polymerase. Transfection of Vero cells with combined plus-sense transcripts of both segments generated infectious virus as early as 36 hours post-transfection. The development of a reverse genetics system for dsRNA viruses will greatly facilitate studies of the regulation of viral gene expression pathogenesis, and design of a new generation of live and inactivated vaccines.

摘要翻译： 已经开发了使用衍生自克隆DNA的合成转录物来产生活的Birnavirus如传染性法氏囊病病毒（IBDV），Birnavirdae家族的分段双链（ds）RNA病毒的系统。 构建了独立的全长cDMA克隆，其分别含有IBDV的RNA片段A和B的整个编码区和非编码区。 通过在T7 RNA聚合酶的线性化质粒的病毒转录中产生两个片段的合成RNA。 早期转染后36小时，用两个片段的组合加正义转录物转染Vero细胞产生感染性病毒。 dsRNA病毒反向遗传学系统的开发将大大促进病毒基因表达发病机制的调控研究，并设计新一代的活疫苗和灭活疫苗。

公开(公告)号：EP1255562B1

公开(公告)日：2008-01-16

申请号：EP01921281.0

申请日：2001-02-13

申请人： Vlaams Interuniversitair Instituut voor Biotechnologie vzw.

发明人： REVETS, Hilde ,  CORNELIS, Pierre ,  DE BAETSELIER, Patrick

IPC分类号： A61K39/39

CPC分类号： A61K39/39 ,  A61K2039/523 ,  A61K2039/55544 ,  A61K2039/57 ,  C07K14/21 ,  C07K14/44 ,  C07K16/10 ,  C07K2319/00 ,  Y02A50/41 ,  Y10S424/823 ,  Y10S424/824 ,  Y10S424/825 ,  Y10S424/826 ,  Y10S424/828 ,  Y10S424/829

摘要： The present invention relates to the use of the major OprI lipoprotein of Pseudomonas aeruginosa to elicit a Type-1 immune response towards a heterologous antigen. The invention relates specifically to the use of OprI-antigen fusion proteins to elicit the Type-1 response. More particularly, the present invention is directed to pharmaceutical formulations comprising OprI and/or OprI fusion proteins, optionally together with a suitable excipient, to stimulate the Th1 dependent, cellular immune response.

公开(公告)号：EP0524205B1

公开(公告)日：1997-08-27

申请号：EP91906493.1

申请日：1991-03-28

申请人： THE WELLCOME FOUNDATION LIMITED

发明人： DOUGAN, Gordon Langley Court ,  CHARLES, Ian, George Langley Court ,  HORMAECHE, Carlos, Estenio Pathology Dept. Univ. ,  JOHNSON, Kevin, Stuart Department of Pathology ,  CHATFIELD, Steven, Neville

IPC分类号： C12N1/21

CPC分类号： C12N15/00 ,  A61K39/0275 ,  A61K2039/522 ,  C07K14/255 ,  C12N9/52 ,  Y02A50/482 ,  Y02A50/484 ,  Y10S424/826

摘要： Attenuated microorganism for use in immunoprophylaxis in which the attenuation is brought about by the presence of a mutation in the DNA sequence of the microorganism which encodes, or which regulates the expression of DNA encoding a protein that is produced in response to environmental stress, the microorganism optionally being capable of expressing DNA encoding a heterologous antigen.

公开(公告)号：EP0749321A1

公开(公告)日：1996-12-27

申请号：EP95911683.0

申请日：1995-02-09

申请人： WILLMAR POULTRY COMPANY, INC. ,  Emery, Daryll A. ,  Straub, Darren E. ,  Huisinga, Richard ,  Carlson, Beth A.

发明人： EMERY, Daryll A. ,  STRAUB, Darren E. ,  HUISINGA, Richard ,  CARLSON, Beth A.

IPC分类号： A61K39 ,  A61P31 ,  G01N33

CPC分类号： C07K14/245 ,  A61K39/0258 ,  A61K39/0275 ,  A61K39/102 ,  G01N33/56911 ,  G01N33/56916 ,  Y02A50/474 ,  Y02A50/482 ,  Y10S424/823 ,  Y10S424/824 ,  Y10S424/825 ,  Y10S424/826 ,  Y10S530/825

摘要： The invention provides a vaccine for immunizing poultry and other animals against infection by a gram-negative bacteria, and a method of immunizing an animal using the vaccine. The vaccine may contain purified siderophore receptor proteins derived from a single strain or species of gram-negative bacteria or other organism, which are cross-reactive with siderophores produced by two or more strains, species or genera of gram-negative bacteria. The invention further provides a process for isolating and purifying the siderophore receptor proteins, and for preparing a vaccine containing the proteins. Also provided is a method for diagnosing gram-negative sepsis.

公开(公告)号：EP0625190A1

公开(公告)日：1994-11-23

申请号：EP93922957.0

申请日：1993-10-14

申请人： Akzo Nobel N.V.

发明人： STORM, Paul Karel ,  VAN EMPEL, Paul Cornelius Maria

IPC分类号： A61K ,  C12N ,  C12N1 ,  A01N63 ,  A61K39 ,  A61P31 ,  G01N33 ,  C12R1

CPC分类号： C12R1/01 ,  A61K39/0208 ,  A61K2039/552 ,  Y10S424/826 ,  Y10S435/82 ,  Y10S435/961

摘要： Cette invention concerne une nouvelle maladie respiratoire d'origine bactérienne affectant les volailles et l'identification de l'agent pathogène responsable. Un vaccin dérivé de cet agent a efficacement protégé de la maladie des poulets mis en contact avec les souches de terrain virulentes.

公开(公告)号：EP0959901A4

公开(公告)日：2002-11-04

申请号：EP97936185

申请日：1997-07-31

申请人： UNIV MARYLAND BIOTECH INST

发明人： VAKHARIA VIKRAM N ,  MUNDT EGBERT

IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K39/12 ,  A61P31/12 ,  C07K14/08 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N7/00 ,  C12P21/02 ,  A61K39/38 ,  C12N7/01 ,  C12N7/04 ,  C12N7/06 ,  C12N7/08 ,  C12N15/00 ,  C12P21/04

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K2039/525 ,  C12N2720/10051 ,  Y10S424/816 ,  Y10S424/826

摘要： A system for the generation of live Birnavirus such as infectious bursal disease virus (IBDV), a segmented double-stranded (ds)RNA virus of the Birnavirdae family, using synthetic transcripts derived from cloned DNA has been developed. Independent full-length cDNA clones were constructed which contained the entire coding and non-coding regions of RNA segments A and B of IBDV, respectively. Synthetic RNAs of both segments were produced by in vitro transcription of linearized plasmids with T7 RNA polymerase. Transfection of Vero cells with combined plus-sense transcripts of both segments generated infectious virus as early as 36 hours post-transfection. The development of a reverse genetics system for dsRNA viruses will greatly facilitate studies of the regulation of viral gene expression pathogenesis, and design of a new generation of live and inactivated vaccines.

公开(公告)号：EP0650733B1

公开(公告)日：2002-01-16

申请号：EP94117270.2

申请日：1994-11-02

申请人： AMERICAN CYANAMID COMPANY

发明人： Coloe, Peter John

IPC分类号： A61K39/02 ,  A61K39/112 ,  A61K39/12 ,  C12N15/00

CPC分类号： A61K39/0275 ,  A61K2039/522 ,  A61K2039/545 ,  A61K2039/552 ,  Y02A50/482 ,  Y10S424/826

摘要： The present invention relates generally to modified microorganisms suitable for use as live in ovo vaccines for avian species. The live in ovo vaccines of the present invention are useful for inducing immunity before or immediately after hatching against a virulent form of the modified microorganism or a microorganism immunologically related to the modified microorganism or a virulent organism or virus carrying an antigenic determinant expressed by the modified microorganism in the live vaccine. The subject live in ovo vaccines are particularly efficacious in enhancing the survival rate of newly-hatched poultry birds.

公开(公告)号：EP0414689B1

公开(公告)日：1994-12-07

申请号：EP89902719.7

申请日：1989-02-10

申请人： COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION

发明人： BOYLE, David, Bernard ,  KUMAR, Sharad

IPC分类号： C12N15/86 ,  A61K39/275

CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N2710/24022 ,  C12N2710/24043 ,  Y10S424/816 ,  Y10S424/826

摘要： A gene sequence including a first promoter for the expression of a major early fowlpox virus (FPV) protein. In a preferred aspect, the gene sequence further includes a second promoter for the expression of a late fowlpox virus protein in opposite orientation to said first promoter. The promoter is useful in developing FPV based vectors for the delivery of vaccine antigens preferably to poultry, and as a tool to study the temporal regulation of poxvirus genes. The invention also offers methods useful in the construction of recombinant fowlpox viruses or related avian poxviruses, which methods are characterized by the introduction of foreign DNA into the fowlpox virus or into virus DNA sequences, which sequences are able to use native FPV promoter regions.

公开(公告)号：EP0414689A1

公开(公告)日：1991-03-06

申请号：EP89902719.0

申请日：1989-02-10

申请人： COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION

发明人： BOYLE, David, Bernard ,  KUMAR, Sharad

IPC分类号： C12N15 ,  A61K39 ,  C07K14

CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N2710/24022 ,  C12N2710/24043 ,  Y10S424/816 ,  Y10S424/826

摘要： La présente invention décrit une séquence de gènes comprenant un premier promoteur servant à l'expression d'une grande protéine précoce du virus de la variole des poules. Dans un aspect préféré, la séquence de gènes comporte en outre un second promoteur servant à l'expression d'une protéine tardive du virus de la variole des poules selon une orientation opposée au premier promoteur. Le promoteur est utile dans le développement de vecteurs fondés sur le virus de la variole des poules et servant à l'administration d'antigènes de vaccin de préférence à des volailles et d'outil pour l'étude de la régulation temporelle des gènes du virus de la variole des poules. La présente invention décrit également des procédés qui sont utiles dans la construction de virus recombinants de la variole des poules ou de virus associés de la variole aviaire et qui se caractérisent par l'introduction d'ADN étranger dans le virus de la variole des poules ou dans des séquences d'ADN dudit virus, lesquelles peuvent utiliser des régions promotrices natives du virus de la variole des poules.

公开(公告)号：EP0307976A1

公开(公告)日：1989-03-22

申请号：EP88201623.1

申请日：1988-07-28

申请人： Akzo Nobel N.V.

发明人： Storm, Paul Karel

IPC分类号： A61K39/02

CPC分类号： A61K39/0241 ,  A61K2039/544 ,  A61K2039/552 ,  Y10S424/826

摘要： The present invention is concerned with a nove Mycoplasma vaccine which is avirulent for chickens an is not pathogenic for turkeys. In particular such vaccine contains the Mycoplasma strain Intervet 6/8 (deposit No. I-673 at CNCM, Pasteur Institute, Paris France).

摘要翻译： 本发明涉及一种对禽类无毒的无支原体疫苗，对火鸡不致病。 特别地，这种疫苗含有支原体菌株Intervet 6/8（CNCM的保藏号I-673，法国巴黎巴斯德研究所）。