公开(公告)号：EP1741791A9

公开(公告)日：2009-07-29

申请号：EP04805142.9

申请日：2004-12-03

申请人： UNIVERSITAT AUTONOMA DE BARCELONA

发明人： JUAN OTERO, Manuel, LIRAD-SSR-CTBT ,  CASAMITJANA PONCES, Natàlia, LIRAD-SSR-CTBT ,  PUJOL BORRELL, Ricardo, LIRAD-SSR-CTBT ,  COLOBRAN ORIOL, Roger, LIRAD-SSR-CTBT ,  PALOU RIVERA, Eduardo, LIRAD-SSR-CTBT ,  FANER CANET, Maria Rosa, LIRAD-SSR-CTBT ,  RIBERA CRUSAFONT, Ana, LIRAD-SSR-CTBT

IPC分类号： C12Q1/68

CPC分类号： C07K14/70539 ,  C12Q1/6883 ,  C12Q2600/156

摘要： The invention relates to a method for determining alleles of the 27 allelic group of B locus of human leukocyte antigen (HLA-B27) from a nucleic acid sample and to a kit for performing this method. The kit comprises allele-specific primers for using in real-time polymerase chain reaction (PCR), and allele-specific fluorescently labelled probes or an intercalating agent, for detecting fluorescence signals. The analysis is completed with the determination of the melting temperatures of the amplified nucleic acid sequences. It overcomes some of the limitations of conventional DNA-based typing methods, in terms of time, possibility of automation, and increase in resolution, while reducing the number of PCR reactions. It is useful e.g. for determining susceptibility for a specific disease.

公开(公告)号：EP1741791A2

公开(公告)日：2007-01-10

申请号：EP04805142.9

申请日：2004-12-03

申请人： UNIVERSITAT AUTONOMA DE BARCELONA

发明人： JUAN OTERO, Manuel, LIRAD-SSR-CTBT ,  CASAMITJANA PONCES, Natàlia, LIRAD-SSR-CTBT ,  PUJOL BORRELL, Ricardo, LIRAD-SSR-CTBT ,  COLOBRAN ORIOL, Roger, LIRAD-SSR-CTBT ,  PALOU RIVERA, Eduardo, LIRAD-SSR-CTBT ,  FANER CANET, Maria Rosa, LIRAD-SSR-CTBT ,  RIBERA CRUSAFONT, Ana, LIRAD-SSR-CTBT

IPC分类号： C12Q1/68

CPC分类号： C07K14/70539 ,  C12Q1/6883 ,  C12Q2600/156

摘要： The invention relates to a method for determining alleles of the 27 allelic group of B locus of human leukocyte antigen (HLA-B27) from a nucleic acid sample and to a kit for performing this method. The kit comprises allele-specific primers for using in real-time polymerase chain reaction (PCR), and allele-specific fluorescently labelled probes or an intercalating agent, for detecting fluorescence signals. The analysis is completed with the determination of the melting temperatures of the amplified nucleic acid sequences. It overcomes some of the limitations of conventional DNA-based typing methods, in terms of time, possibility of automation, and increase in resolution, while reducing the number of PCR reactions. It is useful e.g. for determining susceptibility for a specific disease.

摘要翻译： 本发明涉及一种用于确定来自核酸样品的人类白细胞抗原（HLA-B27）的B基因座的等位基因的等位基因和用于进行该方法的试剂盒的方法。 该试剂盒包括用于实时聚合酶链反应（PCR）的等位基因特异性引物和用于检测荧光信号的等位基因特异性荧光标记探针或插入剂。 通过确定扩增的核酸序列的解链温度来完成分析。 克服了传统的基于DNA的打字方法的一些局限性，从时间，自动化的可能性和分辨率的提高，同时减少了PCR反应的数量。 这是有用的 用于确定特定疾病的易感性。