公开(公告)号：EP2106446A1

公开(公告)日：2009-10-07

申请号：EP08700513.8

申请日：2008-01-10

申请人： University of Saskatchewan

发明人： GEYER, Ronald, C.

IPC分类号： C12N15/62 ,  C07K16/00 ,  C07K16/46 ,  C07K19/00 ,  C07K7/64 ,  C12N15/11 ,  C12N15/13 ,  C12N15/52 ,  C12N9/00 ,  C12P21/00 ,  C12P21/04 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/68

CPC分类号： C07K16/00 ,  C07K2317/565 ,  C07K2317/622 ,  G01N33/5008

摘要： In various aspects, the invention provides methods for cyclizing proteins, including methods for enhancing the stability of cyclized proteins under cytosolic conditions. The invention also provides various methods for using the cyclized proteins. For example, cyclized proteins of the invention may be used in screening assays analogous to the yeast two hybrid assay. Selected embodiments of the invention provide cyclized single chain variable fragment (ScFv) molecules, including molecules in the form of an immunoglobulin fold.