公开(公告)号：EP0498920B1

公开(公告)日：2000-01-26

申请号：EP91107960.6

申请日：1991-05-16

申请人： VICAM, L.P.

发明人： Benjamin, Thomas L. ,  Chen-Wu, Joan ,  Hansen, Thomsen ,  Jackson, Barbara ,  Livingstone, David ,  Tannenbaum, Steven ,  Wogan, Gerald

IPC分类号： G01N33/569 ,  C12Q1/04 ,  G01N33/543

CPC分类号： G01N33/54326 ,  C12Q1/04 ,  C12Q1/24 ,  C12Q1/6804 ,  G01N33/54313 ,  G01N33/569 ,  G01N33/56911

公开(公告)号：EP0605003A3

公开(公告)日：1995-06-07

申请号：EP93121082.7

申请日：1993-12-29

申请人： VICAM, L.P.

发明人： Benjamin, Thomas L. ,  Chen-Wu, Joan ,  Hansen, Thomas ,  Wogan, Gerald ,  Jackson, Barbara ,  Livingston, David ,  Tannenbaum, Steven

IPC分类号： C12Q1/04 ,  C12Q1/68 ,  G01N33/543 ,  G01N33/569 ,  C12Q1/24

CPC分类号： C12Q1/689 ,  C12Q1/04 ,  C12Q1/24 ,  C12Q1/6804 ,  G01N33/54313 ,  G01N33/54326 ,  G01N33/569 ,  G01N33/56911

摘要： An assay method is provided to easily and quickly detect the presence of organisms capable of being cultured, such as bacteria, characterized by antibody capture of the organism of interest by use of specialized magnetic beads, incubation of the captured cells to form colonies; removal of material from the colonies with a colony lift membrane; and detection of the colony material on the membrane sheet by use of labeled antibodies, PCR or nucleic acid probes.

公开(公告)号：EP0605003A2

公开(公告)日：1994-07-06

申请号：EP93121082.7

申请日：1993-12-29

申请人： VICAM, L.P.

发明人： Benjamin, Thomas L. ,  Chen-Wu, Joan ,  Hansen, Thomas ,  Wogan, Gerald ,  Jackson, Barbara ,  Livingston, David ,  Tannenbaum, Steven

IPC分类号： C12Q1/04 ,  C12Q1/68 ,  G01N33/543 ,  G01N33/569 ,  C12Q1/24

CPC分类号： C12Q1/689 ,  C12Q1/04 ,  C12Q1/24 ,  C12Q1/6804 ,  G01N33/54313 ,  G01N33/54326 ,  G01N33/569 ,  G01N33/56911

摘要： An assay method is provided to easily and quickly detect the presence of organisms capable of being cultured, such as bacteria, characterized by antibody capture of the organism of interest by use of specialized magnetic beads, incubation of the captured cells to form colonies; removal of material from the colonies with a colony lift membrane; and detection of the colony material on the membrane sheet by use of labeled antibodies, PCR or nucleic acid probes.

摘要翻译： 提供测定方法以容易且快速地检测能够培养的生物体的存在，例如细菌，其特征在于通过使用专门的磁珠抗体捕获所关注的生物体，孵育所捕获的细胞以形成菌落; 用菌落提取膜从菌落中去除物质; 以及通过使用标记抗体，PCR或核酸探针检测膜片上的菌落物质。

公开(公告)号：EP0498920A3

公开(公告)日：1993-06-16

申请号：EP91107960.6

申请日：1991-05-16

申请人： VICAM, L.P.

发明人： Benjamin, Thomas L. ,  Chen-Wu, Joan ,  Hansen, Thomsen ,  Jackson, Barbara ,  Livingstone, David ,  Tannenbaum, Steven ,  Wogan, Gerald

IPC分类号： G01N33/569 ,  C12Q1/04 ,  G01N33/543

CPC分类号： G01N33/54326 ,  C12Q1/04 ,  C12Q1/24 ,  C12Q1/6804 ,  G01N33/54313 ,  G01N33/569 ,  G01N33/56911

摘要： An assay method is provided to easily and visually detect the presence of organisms capable of being cultured, such as bacteria, characterized by antibody capture of the organism of interest, incubation of the captured cells to form colonies; removal of material from the colonies with a colony lift membrane; and detection of the colony material on the membrane sheet by use of labeled antibodies.

公开(公告)号：EP0498920A2

公开(公告)日：1992-08-19

申请号：EP91107960.6

申请日：1991-05-16

申请人： VICAM, L.P.

发明人： Benjamin, Thomas L. ,  Chen-Wu, Joan ,  Hansen, Thomsen ,  Jackson, Barbara ,  Livingstone, David ,  Tannenbaum, Steven ,  Wogan, Gerald

IPC分类号： G01N33/569 ,  C12Q1/04 ,  G01N33/543

CPC分类号： G01N33/54326 ,  C12Q1/04 ,  C12Q1/24 ,  C12Q1/6804 ,  G01N33/54313 ,  G01N33/569 ,  G01N33/56911

摘要： An assay method is provided to easily and visually detect the presence of organisms capable of being cultured, such as bacteria, characterized by antibody capture of the organism of interest, incubation of the captured cells to form colonies; removal of material from the colonies with a colony lift membrane; and detection of the colony material on the membrane sheet by use of labeled antibodies.

摘要翻译： 提供了一种测定方法，用于容易地和目视地检测能够培养的生物体的存在，例如细菌，其特征在于感兴趣的生物体的抗体捕获，孵育所捕获的细胞以形成菌落; 用菌落提取膜从菌落中去除物质; 以及通过使用标记的抗体检测膜片上的菌落物质。