公开(公告)号：EP1098966A1

公开(公告)日：2001-05-16

申请号：EP99932304.1

申请日：1999-07-07

申请人： Valentis Inc.

发明人： BRIDENBAUGH, Robert ,  DANG, Warren ,  BUSSEY, Lee

IPC分类号： C12N15/10 ,  C12P19/34

CPC分类号： C07H1/06 ,  C07H21/00 ,  C12N15/1003 ,  C12N15/1017

摘要： Methods are provided for producing highly purified compositions of nucleic acids by using a highly streamlined and readily automated process. The methods use static mixers for lysing cells and precipitating debris, followed by centrifugation and ion exchange chromatography. The process may include a purification step using tangential flow ultrafiltration. A scaleable process for producing pharmaceutical grade plasmid DNA, useful for gene therapy, is provided.