公开(公告)号：EP1892292A3

公开(公告)日：2008-09-24

申请号：EP07075392.6

申请日：2001-05-24

申请人： Verenium Corporation

发明人： Short, Jay M. ,  Gray, Kevin A. ,  Barton, Nelson Robert ,  Garrett, James B. ,  O'Donoghue, Eileen ,  Robertson, Dan

IPC分类号： C12N9/16 ,  C12N15/01 ,  C12N15/82 ,  A23K1/165 ,  A23L1/015 ,  A23L1/03 ,  C12N15/09 ,  C12N15/29 ,  C12N15/55 ,  C12N15/63 ,  C12N15/79 ,  C12N5/10 ,  C12N5/14 ,  A01H5/00 ,  A23K1/00 ,  A23K1/18 ,  A23K1/20 ,  A23L1/30

CPC分类号： A21D8/042 ,  A01K2217/05 ,  A23J3/14 ,  A23K10/30 ,  A23K20/189 ,  A23L5/25 ,  A23L7/107 ,  A23L11/33 ,  A23L29/06 ,  A23V2002/00 ,  A61K38/00 ,  C12N9/16 ,  C12Y301/03008 ,  C12Y301/03026 ,  A23V2300/21

摘要： A purified and modified phytase enzyme from Escherichia coli K12 appA phytase is provided. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytase where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytase rich ingredients.

公开(公告)号：EP1892292A2

公开(公告)日：2008-02-27

申请号：EP07075392.6

申请日：2001-05-24

申请人： Verenium Corporation

发明人： Short, Jay M. ,  Kretz, Keith A. ,  Gray, Kevin A. ,  Barton, Nelson Robert ,  Garrett, James B. ,  O'Donoghue, Eileen

IPC分类号： C12N9/16 ,  C12N15/01 ,  C12N15/82 ,  A23K1/165 ,  A23L1/015 ,  A23L1/03 ,  C12N15/09 ,  C12N15/29 ,  C12N15/55 ,  C12N15/63 ,  C12N15/79 ,  C12N5/10 ,  C12N5/14 ,  A01H5/00 ,  A23K1/00 ,  A23K1/18 ,  A23K1/20 ,  A23L1/30

CPC分类号： A21D8/042 ,  A01K2217/05 ,  A23J3/14 ,  A23K10/30 ,  A23K20/189 ,  A23L5/25 ,  A23L7/107 ,  A23L11/33 ,  A23L29/06 ,  A23V2002/00 ,  A61K38/00 ,  C12N9/16 ,  C12Y301/03008 ,  C12Y301/03026 ,  A23V2300/21

摘要： A purified and modified phytase enzyme from Escherichia coli K12 appA phytase is provided. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytase where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytase rich ingredients.

摘要翻译： 提供了来自大肠杆菌K12 appA植酸酶的纯化和修饰的植酸酶。 与野生型酶相比，该酶具有植酸酶活性和改善的耐热性。 此外，酶在低pH下具有改善的蛋白酶稳定性。 修饰的植酸酶的糖基化提供了具有改善的热耐受性和蛋白酶稳定性的进一步改进的酶。 该酶可以由天然的或重组的宿主细胞产生，并且可以用于有助于在需要时消化植酸酶。 特别地，本发明的植酸酶可用于食品中以改善富含植酸酶的成分的摄食量。

公开(公告)号：EP2468853A1

公开(公告)日：2012-06-27

申请号：EP12150400.5

申请日：2005-06-14

申请人： Verenium Corporation

发明人： Lam, David ,  Weiner, David ,  Hitchman, Timothy ,  Barton, Nelson Robert ,  Burk, Mark J.

IPC分类号： C12N9/18 ,  A23L1/27 ,  C11B3/00

CPC分类号： C11B3/003 ,  A23K10/14 ,  A23L5/49 ,  C12N9/18 ,  C12Y301/01014

摘要： The invention provides the invention provides compositions and methods for the enzymatic treatment ("bleaching" or "de-colorizing") of chlorophyll-comprising compositions, e.g., algae preparations, chlorophyll-containing or chlorophyll-contaminated feeds, foods or oils, for example, vegetable oils, including oils processed from oilseeds, such as canola (rapeseed) oil or soybean oil, or oil fruits, such as palm oil. In one aspect, the invention provides methods using a chlorophyllase enzyme for the enzymatic hydrolysis of chlorophyll in an algae, an animal (e.g., a fish) or plant preparation, a food or an oil. In one aspect, the chlorophyllase is immobilized onto a silica. The invention also provides compositions of manufacture and detergents.

摘要翻译： 本发明提供了本发明提供了包含叶绿素的组合物（例如藻类制剂，含叶绿素或叶绿素污染的饲料，食品或油）的酶处理（“漂白”或“脱色”）的组合物和方法，例如 植物油，包括从油籽加工的油，例如油菜籽油菜籽油或大豆油，或油果如棕榈油。 一方面，本发明提供了使用叶绿素酶在藻类，动物（例如鱼）或植物制剂，食物或油中的酶水解叶绿素的方法。 一方面，将叶绿素酶固定在二氧化硅上。 本发明还提供了制造和洗涤剂的组合物。