公开(公告)号：EP2039762A3

公开(公告)日：2009-07-29

申请号：EP08075753.7

申请日：2002-05-15

申请人： Verenium Corporation

发明人： Desantis, Grace ,  Chaplin, Jennifer, Ann ,  Weiner, David, Paul ,  Milan, Aileen ,  Chi, Ellen ,  Short, Jay, M. ,  Madden, Mark ,  Burk, Mark ,  Robertson, Dan

IPC分类号： C12N9/00 ,  C12N9/78 ,  C12N1/20 ,  C12N15/00 ,  C07H21/02 ,  C12P1/00 ,  C12P7/00 ,  C12P7/62 ,  C07K1/00

CPC分类号： C12P13/004 ,  C12N9/78 ,  C12P7/42 ,  C12P13/04 ,  C12P41/006

摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition, methods of designing new nitrilases and methods of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 此外，还提供了设计新的腈水解酶的方法及其使用方法。 腈水解酶在提高的pH和温度下具有增加的活性和稳定性。

公开(公告)号：EP2327766A1

公开(公告)日：2011-06-01

申请号：EP10190397.9

申请日：2002-05-15

申请人： Verenium Corporation

发明人： Chaplin, Jennifer Ann ,  Weiner, David Paul ,  Milan, Aileen ,  Chi, Ellen ,  Short, Jay M ,  Madden, Mark ,  Burk, Mark ,  Robertson, Dan ,  Desantis, Grace

IPC分类号： C12N9/00 ,  C12N9/78 ,  C12N1/20 ,  C12N15/00 ,  C07H21/02 ,  C12P1/00 ,  C12P7/00 ,  C12P7/62 ,  C07K1/00

CPC分类号： C12P13/004 ,  C12N9/78 ,  C12P7/42 ,  C12P13/04 ,  C12P41/006

摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and methods of use therefore are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

公开(公告)号：EP2327765A1

公开(公告)日：2011-06-01

申请号：EP10189100.0

申请日：2002-05-15

申请人： Verenium Corporation

发明人： Chaplin, Jennifer Ann ,  Weiner, David Paul ,  Milan, Aileen ,  Chi, Ellen ,  Short, Jay M ,  Madden, Mark ,  Burk, Mark ,  Robertson, Dan ,  Desantis, Grace

IPC分类号： C12N9/00 ,  C12N9/78 ,  C12N1/20 ,  C12N15/00 ,  C07H21/02 ,  C12P1/00 ,  C12P7/00 ,  C12P7/62 ,  C07K1/00

CPC分类号： C12P13/004 ,  C12N9/78 ,  C12P7/42 ,  C12P13/04 ,  C12P41/006

摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and methods of use therefore are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 此外还提供了设计新的腈水解酶的方法和使用方法。 腈水解酶在提高的pH和温度下具有增加的活性和稳定性。

公开(公告)号：EP2319919A1

公开(公告)日：2011-05-11

申请号：EP10190401.9

申请日：2002-05-15

申请人： Verenium Corporation

发明人： Chaplin, Jennifer Ann ,  Weiner, David Paul ,  Milan, Aileen ,  Chi, Ellen ,  Short, Jay M ,  Madden, Mark ,  Burk, Mark ,  Robertson, Dan ,  Desantis, Grace

IPC分类号： C12N9/00 ,  C12N9/78 ,  C12N1/20 ,  C12N15/00 ,  C07H21/02 ,  C12P1/00 ,  C12P7/00 ,  C12P7/62 ,  C07K1/00

CPC分类号： C12P13/004 ,  C12N9/78 ,  C12P7/42 ,  C12P13/04 ,  C12P41/006

摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and methods of use therefore are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

公开(公告)号：EP1892292A3

公开(公告)日：2008-09-24

申请号：EP07075392.6

申请日：2001-05-24

申请人： Verenium Corporation

发明人： Short, Jay M. ,  Gray, Kevin A. ,  Barton, Nelson Robert ,  Garrett, James B. ,  O'Donoghue, Eileen ,  Robertson, Dan

IPC分类号： C12N9/16 ,  C12N15/01 ,  C12N15/82 ,  A23K1/165 ,  A23L1/015 ,  A23L1/03 ,  C12N15/09 ,  C12N15/29 ,  C12N15/55 ,  C12N15/63 ,  C12N15/79 ,  C12N5/10 ,  C12N5/14 ,  A01H5/00 ,  A23K1/00 ,  A23K1/18 ,  A23K1/20 ,  A23L1/30

CPC分类号： A21D8/042 ,  A01K2217/05 ,  A23J3/14 ,  A23K10/30 ,  A23K20/189 ,  A23L5/25 ,  A23L7/107 ,  A23L11/33 ,  A23L29/06 ,  A23V2002/00 ,  A61K38/00 ,  C12N9/16 ,  C12Y301/03008 ,  C12Y301/03026 ,  A23V2300/21

摘要： A purified and modified phytase enzyme from Escherichia coli K12 appA phytase is provided. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytase where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytase rich ingredients.

公开(公告)号：EP2434008A1

公开(公告)日：2012-03-28

申请号：EP11194671.1

申请日：2007-08-31

申请人： Verenium Corporation ,  BASF SE

发明人： Kerovuo, Janne Samuli ,  Haremza, Sylke ,  Koch, Oliver ,  Habicher, Tilo ,  Robertson, Dan ,  Desantis, Grace ,  Luginbuhl, Peter ,  McCann, Ryan

IPC分类号： C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/68 ,  C12P21/06 ,  C12P21/04 ,  A61K38/44 ,  C07H21/04 ,  C07K1/00

CPC分类号： C12N9/0061 ,  C12N15/09 ,  C12P5/00 ,  C12P7/00 ,  C12P19/00 ,  C12Y110/03002 ,  D21C5/005 ,  D21C9/005 ,  D21C9/1063

摘要： Provided herein are isolated laccase enzymes and nucleic acids encoding them. Also provided are mediators for laccase reactions. Also provided herein are methods for using laccases to oxidize lignins and other phenolic and aromatic compounds, such as for bio-bleaching and decolorization of wood pulp under high temperature and pH conditions to facilitate a substantial reduction in use of bleaching chemicals, as well as for treatment of fibers.

摘要翻译： 本文提供了分离的漆酶和编码它们的核酸。 还提供了漆酶反应的介质。 本文还提供了使用漆酶氧化木质素和其它酚类和芳族化合物的方法，例如在高温和pH条件下用于木浆的生物漂白和脱色，以促进漂白化学品的使用的显着减少，以及用于 处理纤维。

公开(公告)号：EP2327767A1

公开(公告)日：2011-06-01

申请号：EP10190399.5

申请日：2002-05-15

申请人： Verenium Corporation

发明人： Chaplin, Jennifer Ann ,  Weiner, David Paul ,  Milan, Aileen ,  Chi, Ellen ,  Short, Jay M ,  Madden, Mark ,  Burk, Mark ,  Robertson, Dan ,  Desantis, Grace

IPC分类号： C12N9/00 ,  C12N9/78 ,  C12N1/20 ,  C12N15/00 ,  C07H21/02 ,  C12P1/00 ,  C12P7/00 ,  C12P7/62 ,  C07K1/00

CPC分类号： C12P13/004 ,  C12N9/78 ,  C12P7/42 ,  C12P13/04 ,  C12P41/006

摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and methods of use therefore are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提​​供了设计新腈水解酶的方法和使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。

公开(公告)号：EP2258840A1

公开(公告)日：2010-12-08

申请号：EP10177452.9

申请日：2000-12-29

申请人： Verenium Corporation

发明人： Madden, Mark ,  Weiner, David Paul ,  Chaplin, Jennifer Ann ,  Robertson, Dan

IPC分类号： C12N9/78 ,  C12P13/04 ,  C12P7/42 ,  C12P41/00

CPC分类号： C12N9/78 ,  C12N15/00 ,  C12P7/42 ,  C12P13/002 ,  C12P13/04 ,  C12P41/006 ,  C12Y305/05001 ,  Y02P20/52

摘要： In accordance with the present invention there are provided methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids, said method comprising an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity which stereoselectively hydrolyzes the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.

摘要翻译： 根据本发明，提供了制备对映体纯的±取代的羧酸的方法，例如± - 氨基酸和± - 羟酸，所述方法包括具有氰化物和氨的醛或酮或 铵盐或胺，在腈水解酶或具有腈水解酶活性的多肽的存在下，在足以产生羧酸的条件下立即选择性水解氨基腈或氰醇中间体。

公开(公告)号：EP2039762A2

公开(公告)日：2009-03-25

申请号：EP08075753.7

申请日：2002-05-15

申请人： Verenium Corporation

发明人： Desantis, Grace ,  Chaplin, Jennifer, Ann ,  Weiner, David, Paul ,  Milan, Aileen ,  Chi, Ellen ,  Short, Jay, M. ,  Madden, Mark ,  Burk, Mark ,  Robertson, Dan

IPC分类号： C12N9/00 ,  C12N9/78 ,  C12N1/20 ,  C12N15/00 ,  C07H21/02 ,  C12P1/00 ,  C12P7/00 ,  C12P7/62 ,  C07K1/00

CPC分类号： C12P13/004 ,  C12N9/78 ,  C12P7/42 ,  C12P13/04 ,  C12P41/006

摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition, methods of designing new nitrilases and methods of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 此外，还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。