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1.发明授权METHOD FOR DETECTING DNA HAVING MICROSATELLITE REGION 有权VERFAHREN ZUM NACHWEIS VON DNA MIT MIKROSATELLITENREGION
公开(公告)号：EP2840136B1
公开(公告)日：2017-02-08
申请号：EP13764078.5
申请日：2013-03-22
CPC分类号： C12Q1/6876 , C12Q2600/156
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公开(公告)号：EP2426488B1
公开(公告)日：2016-04-20
申请号：EP10769742.7
申请日：2010-04-27
IPC分类号： G01N27/447
CPC分类号： G01N27/44704
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公开(公告)号：EP2075586A1
公开(公告)日：2009-07-01
申请号：EP07807760.9
申请日：2007-09-21
IPC分类号： G01N35/10
CPC分类号： G01N35/1016 , G01N21/11 , G01N35/028 , G01N2035/1034
摘要： A dispensing device for a microchip, which discharges a liquid while preventing formation of a layer of air in a well without using a complicated control mechanism. The dispensing device discharges a liquid (F) into a well (11) of a microchip (10). The microchip (10) includes the well (11), which has an opening (11a) at the upper end thereof and is able to contain the liquid (F), and a microchannel (13a), which is in fluid communication with the lower end of the well (11). The well (11) has a well bottom portion (14a) formed by an annular step portion projecting inward at the lower end. The dispensing device discharges the liquid (F) from the tip opening (2a) after the dispensing nozzle (2) has been moved down until the tip opening (2a) is positioned in the well bottom portion.
摘要翻译： 一种用于微芯片的分配装置，其排出液体，同时防止在井中形成空气层而不使用复杂的控制机构。 分配装置将液体（F）排放到微芯片（10）的井（11）中。 微芯片（10）包括井（11），其在其上端具有开口（11a）并且能够容纳液体（F），以及与下部流体连通的微通道（13a） 井的端部（11）。 井（11）具有由在下端向内突出的环状台阶部形成的井底部（14a）。 在分配喷嘴（2）已经向下移动直到尖端开口（2a）定位在井底部分中之后，分配装置从尖端开口（2a）排出液体（F）。
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4.发明公开METHOD FOR IDENTIFICATION AND DETECTION OF MUTANT GENE USING INTERCALATOR 有权ERKENNUNG MUTIERENDER基因遗传病患者的VERFAHREN ZUR鉴定EINEM INTERKALATOR
公开(公告)号：EP2840137A1
公开(公告)日：2015-02-25
申请号：EP13764366.4
申请日：2013-03-22
CPC分类号： C12Q1/6823 , C12Q1/6827 , G01N2458/30 , C12Q2563/173 , C12Q2565/125 , C12Q2565/131 , C12Q2565/133
摘要： The present invention addresses the problem of improving separation performance in separating a hybrid of a mutant-type DNA and a probe or/and a hybrid of a wild-type type DNA and a probe, said hybrids being obtained by the LH method.
The present invention relates to "a method for detection of mutant-type DNA or/and wild-type DNA which comprises the following steps: (1) a step in which at least one of a single-stranded DNA having a substituted nucleotide, a deficient nucleotide region, or an inserted nucleotide region (mutant-type DNA), or/and a wild-type single-stranded DNA corresponding to the mutant-type DNA (wild-type DNA) is contacted with a probe hybridizing with both single-stranded DNAs, to form a hybrid with the mutant-type DNA (mutant-type hybrid) or/and a hybrid with the wild-type DNA (wild-type hybrid) (in this regard however, at least one of the obtained mutant-type hybrid and wild-type hybrid has a loop structure), (2) a step in which the obtained mutant-type hybrid or/and wild-type hybrid is contacted with an intercalator, and (3) a step in which the presence or absence of the mutant-type DNA or/and the wild-type DNA is detected by separating the conjugate of mutant-type hybrid and intercalator or/and the conjugate of wild-type hybrid and intercalator".摘要翻译： 本发明解决了通过LH法获得的突变型DNA与探针或/和野生型型DNA与探针杂交体的杂交体分离性能提高的问题。 本发明涉及“突变型DNA或/和野生型DNA的检测方法”，其包括以下步骤：（1）将具有取代核苷酸的单链DNA， 与突变型DNA（野生型DNA）相对应的野生型单链DNA（或野生型DNA）与插入的核苷酸区域（突变型DNA）或插入的核苷酸区域（突变型DNA） 与突变型DNA（突变型杂合体）或/和野生型DNA（野生型杂种）的杂交体形成杂交体（然而，在这方面，至少一种获得的突变型DNA） 型杂合体和野生型杂合体具有环结构），（2）所获得的突变型杂合体或/和野生型杂合体与嵌入剂接触的步骤，和（3） 突变型DNA或/和野生型DNA的缺失通过分离突变型杂合体和嵌入剂的共轭物或/和 野生型杂种和嵌入剂的结合物“。
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公开(公告)号：EP2073018A1
公开(公告)日：2009-06-24
申请号：EP07807759.1
申请日：2007-09-21
IPC分类号： G01N35/10
CPC分类号： G01N35/1016 , G01N35/028 , G01N2035/00158 , G01N2035/1034
摘要： A liquid suction device for efficiently suctioning a liquid remaining at an edge portion of the bottom surface of a well. The liquid suction device used to suction a liquid contained in a well (8) of a microchip (8') which includes the well (8) and a microchannel (82a) in fluid communication with the well (8). The liquid suction device includes: a suction nozzle (2) having a tip opening (2a) at a tip thereof and being connected to piping at a rear end thereof, the suction nozzle (2) suctioning the liquid through the tip opening (2a) ; a pump connected to the piping, the pump supplying a suction pressure to the suction nozzle (2) ; and a driving unit for moving the suction nozzle (2) relative to the well (8) . The suction nozzle (2) is moved by the driving unit until the tip opening (2a) contacts a bottom surface of the well (8) while the suction nozzle (2) applies a suction force produced by the suction pressure supplied by the pump, thereby enabling suction of the liquid from the well (8) and/or the microchannel (82a).
摘要翻译： 一种液体抽吸装置，用于有效地抽吸残留在井底表面边缘部分的液体。 用于抽吸容纳在包括井（8）和与井（8）流体连通的微通道（82a）的微芯片（8'）的井（8）中的液体的液体抽吸装置。 该吸液装置包括：在前端具有前端开口（2a）并与后端的配管连接的吸嘴（2），吸嘴（2）通过前端开口（2a）吸引液体， ; 连接到所述管道的泵，所述泵向所述吸嘴（2）供应吸入压力; 以及用于使吸嘴（2）相对于井（8）移动的驱动单元。 通过驱动单元使吸嘴（2）移动，直到吸嘴（2）施加由泵供应的吸入压力产生的吸力时，吸头开口（2a）接触井（8）的底面， 从而能够从井（8）和/或微通道（82a）抽吸液体。
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公开(公告)号：EP2840136A1
公开(公告)日：2015-02-25
申请号：EP13764078.5
申请日：2013-03-22
CPC分类号： C12Q1/6876 , C12Q2600/156
摘要： The problem of the present invention is to provide a method which DNA having a microsatellite region can be accurately detected without causing the problem of non-specific reaction product.
The present invention relates to: "a method for detecting DNA having a microsatellite region by (1) contacting a probe, which does not have a nucleotide sequence complementary to said microsatellite region and hybridizes with both sides of nucleotide sequences of said microsatellite region, with DNA having the microsatellite region, to form a hybrid of the above-mentioned DNA and the above-mentioned probe, which has a loop structure including microsatellite region, (2) separating the obtained hybrid, (3) detecting said hybrid" and "a hybrid of DNA and a probe, having a loop structure including the microsatellite region, which is made by contacting DNA having microsatellite region with the probe which does not have the nucleotide sequence complementary to said microsatellite region, and hybridizes with both sides of nucleotide sequence of said microsatellite region".-
公开(公告)号：EP2194385A1
公开(公告)日：2010-06-09
申请号：EP08833456.0
申请日：2008-09-24
发明人： DENAWA, Tatsuyuki , HAYASHI, Masayoshi , KUROSAWA, Tatsuo , SATOMURA, Masahiro , WATANABE, Mitsuo
IPC分类号： G01N35/02
CPC分类号： G01N35/1002 , B01L3/523 , B01L3/527 , B01L2200/026 , B01L2200/16 , B01L2300/044 , B01L2300/0672 , B01L2300/0861 , G01N35/026 , G01N35/1079 , G01N2035/0436
摘要： A reagent container configured not to require a specific strength for a probe, reduce contamination of the probe and the like, allow accurate liquid level detection, and to be opened easily. A reagent container (50) includes a plurality of open holding sections (52a, 52b, 52c, 52d), each holding a reagent and into which a probe is inserted to suck the reagent. An opening (66) of each of the holding sections (52a, 52b, 52c, 52d) are sealed with at least one sheet-like seal member (62). The reagent container (50) includes a container body (52) and a lid (54) having a plurality of hollow piercing sections (64), formed on a lower surface of the lid, protruding downward for piercing the seal member at each opening (66), and openings (86), formed in an upper surface of the lid, communicating with piercing sections (64) respectively to allow the probe to be inserted therethrough or withdrawn therefrom.
摘要翻译： 配置为不需要针对探针的比强度的试剂容器，可以减少探针等的污染，允许精确的液面检测，容易地打开。 试剂容器（50）包括多个保持试剂的开放保持部（52a，52b，52c，52d），插入探针以吸引试剂。 每个保持部分（52a，52b，52c，52d）的开口（66）用至少一个片状密封件（62）密封。 试剂容器（50）包括容器主体（52）和具有多个中空刺穿部（64）的盖（54），该盖形成在盖的下表面上，向下突出以在每个开口处刺穿密封构件 66）和形成在盖的上表面中的开口（86），分别与穿刺部分（64）连通，以允许探针插入其中或从中抽出。
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