公开(公告)号：EP2007883B1

公开(公告)日：2011-12-28

申请号：EP07755685.0

申请日：2007-04-19

申请人： Wyeth LLC

发明人： KANG, Yun ,  CUTLER, Mark William ,  OUATTARA, Amadou Affrey ,  SYVERTSEN, Kristen Elissa

IPC分类号： C12N7/02

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/5256 ,  C07K14/005 ,  C12N15/86 ,  C12N2740/16222 ,  C12N2760/20243 ,  C12N2760/20251 ,  C12N2760/20252 ,  Y02A50/464

摘要： A process is described for purifying vesicular stomatitis virus (VSV) from cell culture fluid of a mammalian cell culture infected with VSV, the process comprising: clarifying the cell culture fluid by low-speed centrifugation and recovering the VSV in the supernatant; filtering the supernatant through a 0.2 to 0.45 μm filter and recovering the VSV in the filtered solution; loading the VSV filtered solution onto a anion exchange membrane adsorber equilibrated with a first pH buffered salt solution, eluting the VSV from the anion exchange membrane adsorber with a second pH buffered salt solution and recovering the eluted VSV fractions; purifying the recovered VSV by tangential flow filtration (TFF) using a TFF membrane having a molecular weight cutoff between 300 kDa and 1,000 kDa and recovering the VSV in the retentate, and filtering the VSV retentate through a 0.2 to 0.22 μm filter and recovering the VSV in the filtered solution.