公开(公告)号：EP2597154A1

公开(公告)日：2013-05-29

申请号：EP13153949.6

申请日：2009-10-08

申请人： Von Laer, Dorothee

发明人： Von Laer, Dorothee ,  Heimann, Tsanan

IPC分类号： C12N15/86 ,  A61K35/76 ,  C12N5/10 ,  C12N5/074

CPC分类号： C12N15/86 ,  A61K48/005 ,  C07K14/005 ,  C07K14/52 ,  C07K14/5434 ,  C12N9/1211 ,  C12N2760/10022 ,  C12N2760/10023 ,  C12N2760/10032 ,  C12N2760/10033 ,  C12N2760/10044 ,  C12N2760/10045 ,  C12N2760/10122 ,  C12N2760/20222 ,  C12N2760/20223 ,  C12N2760/20241 ,  C12N2760/20243 ,  C12N2760/20245 ,  C12N2760/20252 ,  C12N2810/6072 ,  C12Y207/01021

摘要： Die Erfindung betrifft rekombinante VSV-Viren und virale Vektoren, welche ein Glykoprotein GP des lymphozytären Choriomeningitisvirus (LCMV) anstelle des G-Proteins des VSV enthalten, Virusproduzentenzellen, welche mit LCMV-GP pseudotypisierte VSV-Virionen produzieren, sowie die Verwendung genannter Vektoren und Zellen zur Therapie solider Tumore, insbesondere Hirntumore.

摘要翻译： 包含编码LCMV的GP的基因并且没有VSV的包膜蛋白G的功能基因的水泡性口炎病毒 - 淋巴细胞性脉络丛脑膜炎病毒 - 糖蛋白（VSV-LCMV-GP）假型载体是新的。 包括以下的独立权利要求：（1）包含至少两个补体的复制VSV载体的VSV-LCMV-GP假型载体系统，其含有n，l，p和m编码N，L，P- 和VSV的基质（M）蛋白，编码LCMV-GP的基因gp和没有编码VSV的G蛋白的功能基因，其中在系统的每个载体中，n，l，p的基因之一 ，m和gp缺失，缺失的基因存在于载体系统的另一个载体中; （2）包含作为包膜蛋白的LCMV的GP-蛋白质的假型VSV病毒体; （3）产生假型VSV病毒粒子的病毒生产细胞; （4）在体外转移细胞中的转基因，包括用假型VSV病毒体转导细胞，其中病毒粒子含有转基因，或使细胞与病毒生产细胞接触。 活动：细胞抑制。 行动机制：无给予。

公开(公告)号：EP2350295B1

公开(公告)日：2013-05-15

申请号：EP09748225.1

申请日：2009-10-08

申请人： Von Laer, Dorothee

发明人： VON LAER, Dorothee ,  HEIMANN, Tsanan

IPC分类号： C12N15/86 ,  A61K35/76 ,  C12N5/10 ,  C12N5/074

CPC分类号： C12N15/86 ,  A61K48/005 ,  C07K14/005 ,  C07K14/52 ,  C07K14/5434 ,  C12N9/1211 ,  C12N2760/10022 ,  C12N2760/10023 ,  C12N2760/10032 ,  C12N2760/10033 ,  C12N2760/10044 ,  C12N2760/10045 ,  C12N2760/10122 ,  C12N2760/20222 ,  C12N2760/20223 ,  C12N2760/20241 ,  C12N2760/20243 ,  C12N2760/20245 ,  C12N2760/20252 ,  C12N2810/6072 ,  C12Y207/01021

公开(公告)号：EP2597154B1

公开(公告)日：2014-09-03

申请号：EP13153949.6

申请日：2009-10-08

申请人： ViraTherapeutics GmbH

发明人： Von Laer, Dorothee ,  Heimann, Tsanan

IPC分类号： C12N15/86 ,  A61K35/76 ,  C12N5/10 ,  C12N5/074

CPC分类号： C12N15/86 ,  A61K48/005 ,  C07K14/005 ,  C07K14/52 ,  C07K14/5434 ,  C12N9/1211 ,  C12N2760/10022 ,  C12N2760/10023 ,  C12N2760/10032 ,  C12N2760/10033 ,  C12N2760/10044 ,  C12N2760/10045 ,  C12N2760/10122 ,  C12N2760/20222 ,  C12N2760/20223 ,  C12N2760/20241 ,  C12N2760/20243 ,  C12N2760/20245 ,  C12N2760/20252 ,  C12N2810/6072 ,  C12Y207/01021

公开(公告)号：EP2007883A2

公开(公告)日：2008-12-31

申请号：EP07755685.0

申请日：2007-04-19

申请人： Wyeth

发明人： KANG, Yun ,  CUTLER, Mark William ,  OUATTARA, Amadou Affrey ,  SYVERTSEN, Kristen Elissa

IPC分类号： C12N7/02

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/5256 ,  C07K14/005 ,  C12N15/86 ,  C12N2740/16222 ,  C12N2760/20243 ,  C12N2760/20251 ,  C12N2760/20252 ,  Y02A50/464

摘要： A process is described for purifying vesicular stomatitis virus (VSV) from cell culture fluid of a mammalian cell culture infected with VSV, the process comprising: clarifying the cell culture fluid by low-speed centrifugation and recovering the VSV in the supernatant; filtering the supernatant through a 0.2 to 0.45 μm filter and recovering the VSV in the filtered solution; loading the VSV filtered solution onto a anion exchange membrane adsorber equilibrated with a first pH buffered salt solution, eluting the VSV from the anion exchange membrane adsorber with a second pH buffered salt solution and recovering the eluted VSV fractions; purifying the recovered VSV by tangential flow filtration (TFF) using a TFF membrane having a molecular weight cutoff between 300 kDa and 1,000 kDa and recovering the VSV in the retentate, and filtering the VSV retentate through a 0.2 to 0.22 μm filter and recovering the VSV in the filtered solution.

公开(公告)号：EP3250550A1

公开(公告)日：2017-12-06

申请号：EP16742609.7

申请日：2016-01-26

申请人： Ottawa Hospital Research Institute ,  University Of Ottawa

发明人： DIALLO, Jean-Simon ,  BODDY, Christopher Noyce ,  DORNAN, Mark ,  KRISHNAN, Ramya ,  ARULANANDAM, Rozanne ,  LE BOEUF, Fabrice ,  SMITH, Jeffrey ,  MACKLIN, Andrew

IPC分类号： C07D207/38 ,  A61P31/12 ,  A61P37/04 ,  C07D237/14 ,  C07D307/60 ,  C07D307/66 ,  C12N5/00 ,  C12N5/07 ,  C12N7/00

CPC分类号： C12N7/00 ,  A61K31/4015 ,  A61K31/402 ,  C07D207/36 ,  C07D207/38 ,  C07D207/456 ,  C07D237/14 ,  C07D307/30 ,  C07D307/58 ,  C07D307/60 ,  C07D307/66 ,  C07D317/64 ,  C07D401/06 ,  C07D403/06 ,  C07D405/04 ,  C07D405/06 ,  C07D405/12 ,  C07D409/06 ,  C07D413/06 ,  C07D417/06 ,  C07D471/04 ,  C12N2710/10334 ,  C12N2710/10351 ,  C12N2710/10352 ,  C12N2710/16651 ,  C12N2710/24151 ,  C12N2750/14151 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16152 ,  C12N2760/20234 ,  C12N2760/20251 ,  C12N2760/20252

摘要： Provided are compounds that enhance the efficacy of viruses by increasing spread of the virus in cells, increasing the titer of virus in cells, or increasing the antigen expression from a virus, gene or trans-gene expression from a virus, or virus protein expression in cells. Other uses, compositions and methods of using same are also provided.

公开(公告)号：EP2225368A1

公开(公告)日：2010-09-08

申请号：EP08868608.4

申请日：2008-12-18

申请人： Wyeth LLC

发明人： PARKS, Christopher, L. ,  WITKO, Susan, E. ,  SIDHU, Maninder, K. ,  JOHNSON, J. Erik ,  HENDRY, Roger, Michael

IPC分类号： C12N7/04 ,  C07K14/145

CPC分类号： C12N7/00 ,  C07K14/005 ,  C12N2760/20222 ,  C12N2760/20252 ,  C12N2760/20261

摘要： A method of producing propagation-defective Vesicular Stomatitis Virus (VSV) in a cell culture is provided. The method involves introducing a plasmid vector encoding an optimized VSV G gene into a cell; expressing VSV G protein from the optimized VSV G gene; and introducing a propagation-defective VSV into the cell expressing the VSV G protein encoded by the optimized VSV G gene. The method further includes growing the cells in culture; and recovering the propagation-defective VSV from the culture.

公开(公告)号：EP2225264A1

公开(公告)日：2010-09-08

申请号：EP08867848.7

申请日：2008-12-18

申请人： Wyeth LLC

发明人： PARKS, Christopher, L. ,  WITKO, Susan, E. ,  SIDHU, Maninder, K. ,  JOHNSON, J., Erik ,  HENDRY, Roger, M.

IPC分类号： C07K14/145 ,  C12N7/04 ,  C12N15/63

CPC分类号： C12N7/00 ,  C12N2510/02 ,  C12N2760/20251 ,  C12N2760/20252

摘要： A method of producing propagation-defective Vesicular Stomatitis Virus (VSV) is provided. The method involves providing a cell that includes an optimized VSV G gene, wherein expression of VSV G protein from the optimized VSV G gene is inducible; and inducing the cell to express VSV G protein from the optimized VSV G gene. The method also involves infecting the induced cell with an attenuated VSV; growing the infected cells in culture; and recovering attenuated VSV from the culture.

公开(公告)号：EP2007883B1

公开(公告)日：2011-12-28

申请号：EP07755685.0

申请日：2007-04-19

申请人： Wyeth LLC

发明人： KANG, Yun ,  CUTLER, Mark William ,  OUATTARA, Amadou Affrey ,  SYVERTSEN, Kristen Elissa

IPC分类号： C12N7/02

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/5256 ,  C07K14/005 ,  C12N15/86 ,  C12N2740/16222 ,  C12N2760/20243 ,  C12N2760/20251 ,  C12N2760/20252 ,  Y02A50/464

摘要： A process is described for purifying vesicular stomatitis virus (VSV) from cell culture fluid of a mammalian cell culture infected with VSV, the process comprising: clarifying the cell culture fluid by low-speed centrifugation and recovering the VSV in the supernatant; filtering the supernatant through a 0.2 to 0.45 μm filter and recovering the VSV in the filtered solution; loading the VSV filtered solution onto a anion exchange membrane adsorber equilibrated with a first pH buffered salt solution, eluting the VSV from the anion exchange membrane adsorber with a second pH buffered salt solution and recovering the eluted VSV fractions; purifying the recovered VSV by tangential flow filtration (TFF) using a TFF membrane having a molecular weight cutoff between 300 kDa and 1,000 kDa and recovering the VSV in the retentate, and filtering the VSV retentate through a 0.2 to 0.22 μm filter and recovering the VSV in the filtered solution.

公开(公告)号：EP2350295A1

公开(公告)日：2011-08-03

申请号：EP09748225.1

申请日：2009-10-08

申请人： Von Laer, Dorothee

发明人： LAER, Dorothee von ,  HEIMANN, Tsanan

IPC分类号： C12N15/86 ,  A61K35/76 ,  C12N5/10 ,  C12N5/074

CPC分类号： C12N15/86 ,  A61K48/005 ,  C07K14/005 ,  C07K14/52 ,  C07K14/5434 ,  C12N9/1211 ,  C12N2760/10022 ,  C12N2760/10023 ,  C12N2760/10032 ,  C12N2760/10033 ,  C12N2760/10044 ,  C12N2760/10045 ,  C12N2760/10122 ,  C12N2760/20222 ,  C12N2760/20223 ,  C12N2760/20241 ,  C12N2760/20243 ,  C12N2760/20245 ,  C12N2760/20252 ,  C12N2810/6072 ,  C12Y207/01021

摘要： The invention relates to recombinant VSV viruses and viral vectors which produce a glycoprotein GP of the lymphocytic choriomeningitis virus (LCMV) instead of the G protein of the VSV, to virus-producing cells which produce LCMV-GP-pseudotyped VSV virions, and to the use of said vectors and cells in the therapy of solid tumors, especially brain tumors.

公开(公告)号：EP2298924A2

公开(公告)日：2011-03-23

申请号：EP10174369.8

申请日：2004-06-08

申请人： Wyeth LLC

发明人： Parks, Christopher L. ,  Udem, Stephen A. ,  Sidhu, Modinderjit S.

IPC分类号： C12N15/86 ,  A61K39/12 ,  C12N7/00

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/5258 ,  C12N7/00 ,  C12N15/86 ,  C12N2760/18022 ,  C12N2760/18422 ,  C12N2760/18451 ,  C12N2760/18452 ,  C12N2760/18551 ,  C12N2760/18552 ,  C12N2760/18622 ,  C12N2760/18651 ,  C12N2760/18652 ,  C12N2760/18662 ,  C12N2760/20243 ,  C12N2760/20251 ,  C12N2760/20252 ,  C12N2760/20262

摘要： Methods for producing infectious, non-segmented, negative-stranded RNA viruses of the Order Mononegavirales are provided that involve coexpression of a viral cDNA along with essential viral proteins, N, P, and L in a host cell transiently transfected with an expression vector encoding an RNA polymerase. In alternate methods, after the host cell is transfected with a viral cDNA expression vector and one or more vectors encoding the RNA polymerase, N protein, P protein, and L protein, the host cell is exposed to an effective heat shock under conditions sufficient to increase recovery of the recombinant virus. In other alternate embodiments, the host cells are transferred after viral rescue begins into co-culture with a plaque expansion cell, typically a monolayer of expansion cells, and the assembled infectious, non-segmented, negative-stranded RNA virus is recovered from the co-culture. Also provided within the invention are compositions for producing infectious, non-segmented, negative-stranded RNA virus of the Order Mononegavirales, recombinant viruses produced using the foregoing methods and compositions, and immunogenic compositions and methods employing the recombinant viruses. In additional embodiments, the methods and compositions of the invention are employed to produce growth- or replication-defective non-segmented negative-stranded RNA viruses and subviral particles.

摘要翻译： 提供了用于产生单宁属病毒感染性，非分段的负链RNA病毒的方法，其涉及病毒cDNA与基本病毒蛋白N，P和L共表达在用表达载体编码的瞬时转染的宿主细胞中 RNA聚合酶。 在替代方法中，在用病毒cDNA表达载体和编码RNA聚合酶，N蛋白，P蛋白和L蛋白的一种或多种载体转染宿主细胞后，将宿主细胞暴露于有效的热休克， 增加重组病毒的恢复。 在其他替代实施方案中，宿主细胞在病毒性拯救开始与斑块扩增细胞（通常为单层扩增细胞）共培养后被转移，并且组装的感染性，非分段的负链RNA病毒从共同 -文化。 在本发明中还提供了用于产生单宁属（Mononegavirales）的感染性，非分段的负链RNA病毒的组合物，使用前述方法和组合物产生的重组病毒，以及使用重组病毒的免疫原性组合物和方法。 在另外的实施方案中，本发明的方法和组合物用于产生生长或复制缺陷型非分段负链RNA病毒和亚病毒颗粒。