公开(公告)号：EP4202056A1

公开(公告)日：2023-06-28

申请号：EP21894688.7

申请日：2021-11-17

申请人： Xforest Therapeutics Co., Ltd.

发明人： KOMATSU, Richard Kaoru ,  URTEL, Georg Christian ,  EDELEVA, Evgeniia

IPC分类号： C12Q1/6806 ,  C12N15/09 ,  C40B40/06

摘要： Provided is a method for analyzing a higher-order structure of RNA, the method comprising the steps of: (a) preparing one or a plurality of RNA probes, each comprising an RNA to be analyzed attached to a barcode sequence; (b) contacting the RNA probes and an RNA modification reagent; and (c) detecting a position and a frequency of modified nucleotides in a sequence of the RNA probe obtained in step (b), wherein each of the barcode sequences has a structure that has a reduced reactivity with the RNA modification reagent. The method improves the accuracy of detecting mutations, insertions, and deletions, etc., of a base introduced when carrying out mutation profiling using an RNA library.