RNA REVERSE TRANSCRIPTION AMPLIFICATION METHOD

    公开(公告)号:EP3498861A1

    公开(公告)日:2019-06-19

    申请号:EP17819301.7

    申请日:2017-06-29

    申请人: Xiamen University

    IPC分类号: C12Q1/68

    摘要: A ribonucleic acid (RNA) reverse transcription amplification method, comprising the steps of reverse transcription of RNA into cDNA and immediate amplification of cDNA, characterized in that the process of reverse transcription of RNA into cDNA is completed during the process that a cDNA amplification reaction system is heated to reach the reaction condition for cDNA amplification. The RNA reverse transcription amplification method can combine the reaction condition for reverse transcription of RNA into cDNA with the reaction condition for cDNA amplification, thereby significantly shorten the time required for RNA reverse transcription amplification. And in the whole process of RNA reverse transcription amplification, there is no need to change the instrument temperature, and thus, it is possible to achieve detection at any time.