公开(公告)号：EP1267170A1

公开(公告)日：2002-12-18

申请号：EP01304975.4

申请日：2001-06-07

申请人： Xzillion GmbH & CO.KG

发明人： Schmidt, Gunter ,  Johnstone, Robert Alexander Walker ,  Thompson, Andrew Hugin ,  Hamon, Christian ,  Neumann, Thomas ,  Joubert, Richard

IPC分类号： G01N33/68 ,  C07K1/12

CPC分类号： G01N33/6848 ,  C07K1/12 ,  G01N33/6821

摘要： Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of:

(a) contacting a sample comprising one or more polypeptides with a lysine reactive agent to cap ε-amino groups;
(b) optionally reacting the sample of polypeptides with an amine reactive reagent to block α-amino groups;
(c) digesting the sample of polypeptides with a sequence specific cleavage reagent to produce peptide fragments;
(d) optionally deactivating the cleavage reagent;
(e) removing those peptides having uncapped or unblocked amino groups; and
(f) recovering the N-terminal peptides.

摘要翻译： 提供了表征多肽或多肽群体的方法，该方法包括以下步骤：（a）将包含一种或多种多肽的样品与赖氨酸反应剂接触以将ε-氨基封端; （b）任选地使多肽样品与胺反应试剂反应以阻断α-氨基; （c）用序列特异性裂解试剂消化多肽样品以产生肽片段; （d）任选地使切割试剂失活; （e）除去具有未封端或未封闭氨基的那些肽; 和（f）回收N-末端肽