-
1.发明公开
公开(公告)号：EP1267170A1
公开(公告)日：2002-12-18
申请号：EP01304975.4
申请日：2001-06-07
发明人： Schmidt, Gunter , Johnstone, Robert Alexander Walker , Thompson, Andrew Hugin , Hamon, Christian , Neumann, Thomas , Joubert, Richard
CPC分类号： G01N33/6848 , C07K1/12 , G01N33/6821
摘要： Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of:
(a) contacting a sample comprising one or more polypeptides with a lysine reactive agent to cap ε-amino groups;
(b) optionally reacting the sample of polypeptides with an amine reactive reagent to block α-amino groups;
(c) digesting the sample of polypeptides with a sequence specific cleavage reagent to produce peptide fragments;
(d) optionally deactivating the cleavage reagent;
(e) removing those peptides having uncapped or unblocked amino groups; and
(f) recovering the N-terminal peptides.摘要翻译： 提供了表征多肽或多肽群体的方法，该方法包括以下步骤：（a）将包含一种或多种多肽的样品与赖氨酸反应剂接触以将ε-氨基封端; （b）任选地使多肽样品与胺反应试剂反应以阻断α-氨基; （c）用序列特异性裂解试剂消化多肽样品以产生肽片段; （d）任选地使切割试剂失活; （e）除去具有未封端或未封闭氨基的那些肽; 和（f）回收N-末端肽
-
2.发明公开Silicon-containing linkers for nucleic acid mass markers 审中-公开Silicium enthaltende LinkerfürNukleinsäure-Massmarker
公开(公告)号：EP1275655A1
公开(公告)日：2003-01-15
申请号：EP02021105.8
申请日：1999-07-13
CPC分类号： C07H23/00 , C07H21/00 , C12Q1/6872 , C40B40/00
摘要： Provided is a method for characterising an analyte, which method comprises:
(a) providing a compound in which the analyte is attached by a cleavable linker to a reporter group relatable to the analyte, the linker having the following formula:
wherein R 1 and R 2 are substituents as defined in any of claims 1-4 16 and 17;
(b) cleaving the reporter group from the analyte; and
(c) identifying the reporter group, thereby characterising the analyte.摘要翻译： 提供了一种用于表征分析物的方法，该方法包括：（a）提供其中分析物通过可切割接头连接到与分析物相关的报告基团的化合物，所述接头具有下式：其中R R 1和R 2是如权利要求1-4-16和17中任一项所定义的取代基; （b）从分析物裂解报道基团; 和（c）识别报告基团，从而表征分析物。
-
公开(公告)号：EP1400599B1
公开(公告)日：2007-10-03
申请号：EP03076965.7
申请日：1998-01-15
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6816 , C12Q1/6872 , C12Q1/6874
-
公开(公告)号：EP1400599A1
公开(公告)日：2004-03-24
申请号：EP03076965.7
申请日：1998-01-15
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6816 , C12Q1/6872 , C12Q1/6874
摘要： An array of hybridisation probes, each of which comprises a mass label linked to a known base sequence of predetermined length, wherein each mass label of the array, optionally together with the known base sequence, is relatable to that base sequence by mass spectrometry.
摘要翻译： 杂交探针的阵列，每个探针包含与已知碱基序列连接的质量标签，其具有预定长度的已知碱基序列，其中阵列的每个质量标签（可选地与已知碱基序列一起）可通过质谱法与该碱基序列相关。
-
-
-
搜索结果
4 条记录 (耗时 0.015 秒)
