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公开(公告)号：EP1606623A2
公开(公告)日：2005-12-21
申请号：EP04721565.2
申请日：2004-03-18
CPC分类号： G01N33/68 , C07B2200/05 , C07C229/12 , C07D207/46 , C07D211/14 , C07D295/15 , C07D401/12 , C07K1/13 , C40B40/04 , C40B70/00 , G01N33/6848 , G01N2458/15 , Y10T436/24
摘要： Provided is a method for characterising a molecule by mass spectrometry, which molecule comprises one or more free amino groups, which method comprises: (a) reacting one or more free amino groups in the molecule with a mass tag reagent comprising a reactive functionality capable of reacting with an amino group, and a tertiary amino group linked to the reactive functionality; and (b) characterising the molecule by mass spectrometry.
摘要翻译： 提供了一种通过质谱法表征分子的方法，所述分子包含一个或多个游离氨基，所述方法包括：（a）使分子中的一个或多个游离氨基与包含反应性官能团的质量标签试剂反应，所述反应性官能团能够 与氨基和与反应性官能团连接的叔氨基反应; 和（b）通过质谱法表征分子。
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公开(公告)号：EP1397686A1
公开(公告)日：2004-03-17
申请号：EP02735602.1
申请日：2002-06-07
发明人： HAMON, Christian , THOMPSON, Andrew , NEUMANN, Thomas , JOHNSTONE, Robert , MOHAMMED, Abdul Karim Abed
CPC分类号： G01N33/6848 , C07K1/12 , G01N33/6821
摘要： Provided is a method for characterising a polypeptide, which method comprises the steps of: (a) optionnally reducing cysteine disulphide bridges in the polypeptide to form free thiols, and capping the free thiols; (b) cleaving the polypeptide with a sequence specific cleavage reagent to form peptide fragments; (c) optionally deactivating the cleavage reagent; (d) capping one or more ε-amino groups that are present with a lysine reactive agent; (e) analysing peptide fragments by mass spectrometry to form a mass fingerprint for the polypeptide; and (f) determining the identity of the polypeptide from the mass fingerprint.
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公开(公告)号：EP1397505A2
公开(公告)日：2004-03-17
申请号：EP02735600.5
申请日：2002-06-07
发明人： HAMON, Christian , THOMPSON, Andrew , KUHN, Karsten , NEUMANN, Thomas , JOUBERT, Richard , JOHNSTONE, Robert , SCHMIDT, Gunter
IPC分类号： C12Q1/37 , G01N33/68 , C07D213/71 , C07D215/36 , C07D241/18 , C07D239/38 , C07D251/58 , C07K1/107
CPC分类号： G01N33/6848 , C07K1/12 , G01N33/6821
摘要： Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of: (a) contacting a sample comprising one or more polypeptides with a lysine reactive agent to cap ε-amino groups; (b) optionally reacting the sample of polypeptides with an amine reactive reagent to block α-amino groups; (c) digesting the sample of polypeptides with a cleavage reagent to produce peptide fragments; (d) optionally deactivating the cleavage reagent; (e) removing those peptides having uncapped or unblocked amino groups; and (f) recovering the N-terminal peptides.
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