公开(公告)号：EP1397505A2

公开(公告)日：2004-03-17

申请号：EP02735600.5

申请日：2002-06-07

申请人： Xzillion GmbH & CO.KG

发明人： HAMON, Christian ,  THOMPSON, Andrew ,  KUHN, Karsten ,  NEUMANN, Thomas ,  JOUBERT, Richard ,  JOHNSTONE, Robert ,  SCHMIDT, Gunter

IPC分类号： C12Q1/37 ,  G01N33/68 ,  C07D213/71 ,  C07D215/36 ,  C07D241/18 ,  C07D239/38 ,  C07D251/58 ,  C07K1/107

CPC分类号： G01N33/6848 ,  C07K1/12 ,  G01N33/6821

摘要： Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of: (a) contacting a sample comprising one or more polypeptides with a lysine reactive agent to cap ε-amino groups; (b) optionally reacting the sample of polypeptides with an amine reactive reagent to block α-amino groups; (c) digesting the sample of polypeptides with a cleavage reagent to produce peptide fragments; (d) optionally deactivating the cleavage reagent; (e) removing those peptides having uncapped or unblocked amino groups; and (f) recovering the N-terminal peptides.

公开(公告)号：EP1275004A2

公开(公告)日：2003-01-15

申请号：EP01911912.2

申请日：2001-03-14

申请人： Xzillion GmbH & Co.KG

发明人： SCHMIDT, Gunter ,  THOMPSON, Andrew Hugin ,  JOHNSTONE, Robert Alexander Walker

IPC分类号： G01N33/58 ,  C12Q1/68 ,  G01N33/68

CPC分类号： C07H21/00 ,  C07K1/1077 ,  C12Q1/6872 ,  C40B40/00 ,  G01N33/6803 ,  G01N2458/15 ,  Y10T436/13

摘要： Provided is a set of two or more mass labels, each label in the set comprising a mass marker moiety attached via a cleavable linker to a mass normalisation moiety, the mass marker moiety being fragmentation resistant, wherein the aggregate mass of each label in the set may be the same or different and the mass of the mass marker moiety of each label in the set may be the same or different, and wherein in any group of labels within the set having a mass marker moiety of a common mass each label has an aggregate mass different from all other labels in that group, and wherein in any group of labels within the set having a common aggregate mass each label has a mass marker moiety having a mass different from that of all other mass marker moieties in that group, such that all of the mass labels in the set are distinguishable from each other by mass spectrometry.

公开(公告)号：EP0857208B1

公开(公告)日：2007-07-11

申请号：EP96930282.7

申请日：1996-09-13

申请人： Xzillion GmbH & CO.KG

发明人： SCHMIDT, Gunter

IPC分类号： C12N15/11 ,  C07H21/00 ,  A61K31/70

CPC分类号： C07H21/00

摘要： A chimaeric oligonucleotide library for use in identifying an antisense binding site in a target mRNA, comprising a plurality of distinct chimaeric oligonucleotides capable of hybridizing to mRNA to form a duplex, the nucleotide sequences of which each have a common length of 7 to 20 bases and are generated randomly or generated from information characterising the sequence of the target mRNA, wherein substantially all the nucleotide sequences of said common length which are present as sub-sequences in the target mRNA are present in the library, and wherein each nucleotide sequence comprises: a) a recognition region comprising a sequence of nucleotides which is recognisable by a duplex-cutting RNAase when hybridized to the mRNA, and b) a flanking region comprising a sequence of chemically-modified nucleotides which binds to the mRNA sufficiently tightly to stabilise the duplex for cutting of the mRNA in the duplex by the duplex-cutting RNAase, wherein the nucleotides constituting the flanking region are different from those constituting the recognition region, and wherein each oligonucleotide is protected against exonuclease attack.

公开(公告)号：EP0927267B1

公开(公告)日：2002-04-10

申请号：EP97937754.6

申请日：1997-09-05

申请人： Xzillion GmbH & CO.KG

发明人： SCHMIDT, Gunter ,  THOMPSON, Andrew, Hugin

IPC分类号： C12Q1/68

CPC分类号： C12N15/1096 ,  C12Q1/6837 ,  C12Q2525/191 ,  C12Q2523/107