公开(公告)号：EP1331271A4

公开(公告)日：2005-04-06

申请号：EP01994540

申请日：2001-09-30

申请人： YANG SHENG TANG COMPANY LTD

发明人： XIA NINGSHAO ,  ZHANG JUN ,  LI SHAOWEI ,  GE SHENGXIANG ,  GU YING ,  HE ZHIGIANG

IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K39/29 ,  A61K39/295 ,  A61P1/16 ,  A61P31/12 ,  C07K14/08 ,  C07K14/705 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/51 ,  G01N33/543 ,  G01N33/566 ,  G01N33/576 ,  G01N33/577 ,  C12Q1/70 ,  G01N33/569

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/12 ,  A61K39/29 ,  A61K2039/55505 ,  A61K2039/55566 ,  A61K2039/57 ,  A61K2039/6075 ,  C07K16/10 ,  C07K2319/00 ,  C12N2760/16022 ,  C12N2770/28122 ,  C12N2770/28134 ,  C12N2770/28171 ,  G01N33/576

摘要： The invention relates to n-met of polypeptide comprising the amino acids of ORF-2 of the hepatitis E virus shown by the sequences of SEQ ID NO:1 or fragments thereof, wherein n is integer of 2-180. The invention also relates to the chimeric polypeptide of said fragments fused to conservative fragment of influenza virus hemagglutinin, and to covalent binding of said fragments with epitope polypeptide of ORF3 of hepatitis E virus or its fragments of immungenic activity, and to an expression vector comprising DNA molecules coding for above polypeptide, an expression host comprising expression vector. The invention also relates to vaccine composition and a kit of comprising above said polypeptide of HEV for diagnosing infection of HEV, and to diagnostic kits comprising IgG, IgM and total antibody, and its uses for preventing, diagnosing and/or treating infection of HEV.