公开(公告)号：EP1331271A4

公开(公告)日：2005-04-06

申请号：EP01994540

申请日：2001-09-30

申请人： YANG SHENG TANG COMPANY LTD

发明人： XIA NINGSHAO ,  ZHANG JUN ,  LI SHAOWEI ,  GE SHENGXIANG ,  GU YING ,  HE ZHIGIANG

IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K39/29 ,  A61K39/295 ,  A61P1/16 ,  A61P31/12 ,  C07K14/08 ,  C07K14/705 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/51 ,  G01N33/543 ,  G01N33/566 ,  G01N33/576 ,  G01N33/577 ,  C12Q1/70 ,  G01N33/569

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/12 ,  A61K39/29 ,  A61K2039/55505 ,  A61K2039/55566 ,  A61K2039/57 ,  A61K2039/6075 ,  C07K16/10 ,  C07K2319/00 ,  C12N2760/16022 ,  C12N2770/28122 ,  C12N2770/28134 ,  C12N2770/28171 ,  G01N33/576

摘要： The invention relates to n-met of polypeptide comprising the amino acids of ORF-2 of the hepatitis E virus shown by the sequences of SEQ ID NO:1 or fragments thereof, wherein n is integer of 2-180. The invention also relates to the chimeric polypeptide of said fragments fused to conservative fragment of influenza virus hemagglutinin, and to covalent binding of said fragments with epitope polypeptide of ORF3 of hepatitis E virus or its fragments of immungenic activity, and to an expression vector comprising DNA molecules coding for above polypeptide, an expression host comprising expression vector. The invention also relates to vaccine composition and a kit of comprising above said polypeptide of HEV for diagnosing infection of HEV, and to diagnostic kits comprising IgG, IgM and total antibody, and its uses for preventing, diagnosing and/or treating infection of HEV.

公开(公告)号：EP2174957A4

公开(公告)日：2011-12-14

申请号：EP08772961

申请日：2008-06-16

申请人： UNIV XIAMEN ,  YANG SHENG TANG COMPANY LTD

发明人： CHEN YIXIN ,  LUO WENXIN ,  ZHANG JUN ,  XIA NINGSHAO ,  GUAN YI ,  CHEN HONGLIN

IPC分类号： C07K16/18 ,  A61K38/08 ,  A61K38/10 ,  A61K39/395 ,  A61P31/16 ,  C07K7/06 ,  C07K7/08 ,  C07K14/11 ,  C07K16/10 ,  C07K16/46 ,  C12N5/12 ,  C12N15/13 ,  C12N15/63 ,  G01N33/53 ,  G01N33/569

CPC分类号： C07K16/1018 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/622 ,  C07K2317/76 ,  C12N2760/16111 ,  G01N33/56983 ,  G01N2333/11

公开(公告)号：EP1452541A4

公开(公告)日：2005-07-20

申请号：EP02781056

申请日：2002-11-08

申请人： YANG SHENG TANG COMPANY LTD

发明人： XIA NINGSHAO ,  ZHANG JUN ,  GU YING ,  LI SHAOWEI ,  GE SHENGXIANG ,  HE ZHIQIANG

IPC分类号： G01N33/50 ,  A61K38/00 ,  A61K39/00 ,  A61K39/29 ,  A61K39/395 ,  A61P31/20 ,  C07K14/08 ,  C07K16/08 ,  C07K16/10 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/00 ,  C12N15/02 ,  C12N15/09 ,  C12N15/13 ,  C12P21/08 ,  G01N33/00 ,  G01N33/15 ,  G01N33/543 ,  G01N33/576

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/29 ,  A61K2039/545 ,  A61K2039/55505 ,  A61K2039/55566 ,  A61K2039/57 ,  C07K16/10 ,  C12N2770/28122 ,  C12N2770/28134 ,  G01N33/576

摘要： The present invention relates to monoclonal antibodies which specifically bind to hepatitis E virus (HEV) open reading frame (OFR) 2 (SEQ IN NO:1), to their conserved variants or active fragments, or other monoclonal antibodies which are against OFR2 region and can cross react with the monoclonal antibodies according to the present invention, to the nucleotide sequences encoding them or the degeneration sequences. The present invention also discloses the antigenic determinants on hepatitis E virus OFR2 region. The present invention also discloses methods for screening isolated or recombination polypeptides or peptide mimics, the said polypeptides or peptide mimics have the characters corresponding to that hepatitis E virus OFR2 antigenic determinants 1) or 3) specifically bind to the present monoclonal antibodies 8C11 and 8H3. The present invention also discloses the polypeptides or peptide mimics and the nucleotide sequences encoding them or the degeneration sequences which are screened by the above methods. The present invention also discloses the uses of the polypeptides or peptide mimics for diagnosis and/or prevents of hepatitis E virus infection. In addition, the present invention discloses assay kits for diagnosis of HEV infection, and vaccines for prevent of HEV infection. The present invention further relates to the uses of monoclonal antibodies, the active fragments thereof or their conserved variants for preparing medicines for diagnosis, prevents and/or treatments of hepatitis E virus infection. The present invention relates to pharmaceutical compositions for treating HEV infection, to methods for preventing and/or treating HEV infection. The present invention also relates to recombination express vectors containing the said nucleotide sequences, to the recombination hosts transformed by the recombination express vectors, the recombination hosts can express the present monoclonal antibodies, their conserved variants or the active fragments thereof, the polypeptides or peptide mimics.