公开(公告)号：EP1366362A2

公开(公告)日：2003-12-03

申请号：EP01988862.7

申请日：2001-10-26

申请人： responsif GmbH

发明人： HERRMANN, Martin ,  KALDEN, Joachim, Robert ,  BERTLING, Wolf ,  REISER, Christian ,  GAIPL, Udo ,  HEYDER, Petra ,  WOITH, Walter

IPC分类号： G01N33/50 ,  G01N33/569

CPC分类号： G01N33/56972 ,  G01N33/5091 ,  G01N2333/42 ,  G01N2510/00

摘要： The invention relates to a method for the identification of apoptotic and necrotic cells in a cell suspension, comprising the following steps: a) contacting a first sample taken from said cell suspension with a lectin that is suitable to bind to sugar structures contained in the membrane of apoptotic and necrotic cells; b) acidifying the first sample; c) detecting the amount of lectin bound by the apoptotic and necrotic cells.

公开(公告)号：EP1366362B1

公开(公告)日：2005-11-16

申请号：EP01988862.7

申请日：2001-10-26

申请人： responsif GmbH

发明人： HERRMANN, Martin ,  KALDEN, Joachim, Robert ,  BERTLING, Wolf ,  REISER, Christian ,  GAIPL, Udo ,  HEYDER, Petra ,  WOITH, Walter

IPC分类号： G01N33/50 ,  G01N33/569

CPC分类号： G01N33/56972 ,  G01N33/5091 ,  G01N2333/42 ,  G01N2510/00

摘要： The invention relates to a method for the identification of apoptotic and necrotic cells in a cell suspension, comprising the following steps: a) contacting a first sample taken from said cell suspension with a lectin that is suitable to bind to sugar structures contained in the membrane of apoptotic and necrotic cells; b) acidifying the first sample; c) detecting the amount of lectin bound by the apoptotic and necrotic cells.