公开(公告)号：EP0594669A1

公开(公告)日：1994-05-04

申请号：EP92914286.0

申请日：1992-07-07

申请人： NEUROSPHERES LTD.

发明人： WEISS, Samuel ,  REYNOLDS, Brent, A.

IPC分类号： C12N ,  A61K35 ,  A61K38 ,  A61P25 ,  C07K14 ,  C12N5 ,  C12N9 ,  A61K48

CPC分类号： A61K38/1808 ,  A01K2217/05 ,  A61K35/30 ,  A61K38/1841 ,  A61K48/00 ,  C07K14/47 ,  C07K14/475 ,  C07K14/82 ,  C12N5/0623 ,  C12N9/0065 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/148 ,  C12N2510/00 ,  A61K2300/00

摘要： An in vitro culture system for the perpetuation of an unlimited number of neural progenitor cells. Progenitor cells are isolated from particular neural regions and proliferated in suspension cultures in the presence of growth factors. The progenitor cells can be induced to differentiate into neurons and glial cells. The ability to perpetuate fetal progenitor cells allows for production of a large supply of tissue from a minimal number of fetuses for transplantation into an animal with neurodegeneration. The use of juvenile and adult cells for generating progenitors would eliminate the need to obtain fetal tissue and may allow for patient to supply his own progenitors. Such an approach would eliminate the ethical problem of obtaining fetal neuronal tissue as well as the problem of tissue rejection and the required use of immunosuppressive drugs.

公开(公告)号：EP0681477A4

公开(公告)日：1998-07-15

申请号：EP94907366

申请日：1994-01-28

申请人： NEUROSPHERES LTD

发明人： WEISS SAMUEL ,  REYNOLDS BRENT A ,  HAMMANG JOSEPH P ,  BAETGE EDWARD E

IPC分类号： C12N15/09 ,  A61K20060101 ,  A61K31/70 ,  A61K35/12 ,  A61K35/30 ,  A61K35/54 ,  A61K38/00 ,  A61K38/04 ,  A61K38/17 ,  A61K38/18 ,  A61K38/22 ,  A61K38/27 ,  A61K38/44 ,  A61K48/00 ,  A61P25/00 ,  A61P25/28 ,  C07H21/04 ,  C07K14/47 ,  C07K14/475 ,  C07K14/82 ,  C12N20060101 ,  C12N5/00 ,  C12N5/0797 ,  C12N5/10 ,  C12N9/08 ,  C12N9/38 ,  C12N15/85 ,  C12P21/00

CPC分类号： C12N5/0623 ,  A01K2217/05 ,  A61K35/30 ,  A61K38/179 ,  A61K48/00 ,  C07K14/47 ,  C07K14/475 ,  C07K14/82 ,  C12N9/0065 ,  C12N9/2471 ,  C12N2500/25 ,  C12N2500/46 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/148 ,  C12N2501/392 ,  C12N2510/00 ,  C12Y302/01023 ,  A61K2300/00

摘要： Genetically modified, epidermal growth factor-responsive neural stem cells are disclosed that are capable of differentiating into neurons, astrocytes, and oligodendrocytes in vitro. The stem cells can be proliferated in vitro to result in large numbers of cells for use in transplantation to treat various neurological disorders. The stem cells can be obtained from a human or other animal, cultured in vitro in the presence of a growth factor and genetically modified with desired DNA using techniques known in the art. Alternatively, the precursor cells can be derived from a transgenic animal which has had a desired gene inserted into its genome.

公开(公告)号：EP0681477A1

公开(公告)日：1995-11-15

申请号：EP94907366.0

申请日：1994-01-28

申请人： NEUROSPHERES LTD.

发明人： WEISS, Samuel ,  REYNOLDS, Brent A. ,  HAMMANG, Joseph P. ,  BAETGE, Edward E.

IPC分类号： A61K ,  C12N ,  C12N15 ,  A61K31 ,  A61K35 ,  A61K38 ,  A61K48 ,  A61P25 ,  C07H21 ,  C07K14 ,  C12N5 ,  C12N9 ,  C12P21

CPC分类号： C12N5/0623 ,  A01K2217/05 ,  A61K35/30 ,  A61K38/179 ,  A61K48/00 ,  C07K14/47 ,  C07K14/475 ,  C07K14/82 ,  C12N9/0065 ,  C12N9/2471 ,  C12N2500/25 ,  C12N2500/46 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/148 ,  C12N2501/392 ,  C12N2510/00 ,  C12Y302/01023 ,  A61K2300/00

摘要： Genetically modified, epidermal growth factor-responsive neural stem cells are disclosed that are capable of differentiating into neurons, astrocytes, and oligodendrocytes in vitro. The stem cells can be proliferated in vitro to result in large numbers of cells for use in transplantation to treat various neurological disorders. The stem cells can be obtained from a human or other animal, cultured in vitro in the presence of a growth factor and genetically modified with desired DNA using techniques known in the art. Alternatively, the precursor cells can be derived from a transgenic animal which has had a desired gene inserted into its genome.

公开(公告)号：EP0669973A1

公开(公告)日：1995-09-06

申请号：EP93923994.0

申请日：1993-10-27

申请人： NEUROSPHERES LTD.

发明人： WEISS, Samuel ,  REYNOLDS, Brent, A. ,  HAMMANG, Joseph, P. ,  BAETGE, E. Edward

IPC分类号： A61K38 ,  C07K14 ,  C12N5 ,  C12N9 ,  A61K35 ,  A61K48

CPC分类号： C07K14/82 ,  A01K2217/05 ,  A61K48/00 ,  C07K14/47 ,  C07K14/475 ,  C12N5/0623 ,  C12N9/0065 ,  C12N9/88 ,  C12N2500/25 ,  C12N2500/46 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/148 ,  C12N2501/392 ,  C12N2510/00 ,  C12Y402/01011

摘要： Methods are described for increasing the number of neural stem cells that differentiate into astrocytes, oligodendrocytes, or neurons. The methods comprise proliferating isolated neural stem cells in a culture medium having a first growth factor to produce precursor cells. The precursor cells are then differentiated into astrocytes, oligodendrocytes, or neurons in a second culture medium, free of the first growth factor, containing a second growth factor or combination of growth factors.

公开(公告)号：EP0664832A1

公开(公告)日：1995-08-02

申请号：EP93922482.0

申请日：1993-10-15

申请人： NEUROSPHERES LTD.

发明人： WEISS, Samuel ,  REYNOLDS, Brent, A. ,  HAMMANG, Joseph, P.

IPC分类号： A61K35 ,  A61K38 ,  A61P25 ,  C07K14 ,  C12N5 ,  C12N9 ,  A61K48

CPC分类号： A61K35/30 ,  A01K2217/05 ,  A61K48/00 ,  C07K14/47 ,  C07K14/475 ,  C07K14/82 ,  C12N5/0623 ,  C12N9/0065 ,  C12N2500/25 ,  C12N2500/46 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/392

摘要： A method for the remyelination of neurons is disclosed wherein neural stem cells isolated from adult or fetal neural tissue are proliferated in a culture medium containing a growth factor to produce precursor cells having a nestin (+) phenotype. The precursor cells are capable of differentiation into oligodendrocytes which, when associated with a demyelinated neuron, effect remyelination.