公开(公告)号：EP2585598B1

公开(公告)日：2015-12-30

申请号：EP11798901.2

申请日：2011-06-23

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Services

发明人： WU, Xianfu ,  RUPPRECHT, Charles, E. ,  KUZMIN, Ivan, V.

IPC分类号： C12N15/47 ,  C12N15/63 ,  A61K39/205

CPC分类号： A61K39/205 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/70 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2760/20121 ,  C12N2760/20122 ,  C12N2760/20134 ,  C12N2760/20143 ,  C12N2760/20152 ,  C12N2800/50

公开(公告)号：EP1768697B1

公开(公告)日：2010-06-02

申请号：EP05790962.4

申请日：2005-07-12

申请人： THOMAS JEFFERSON UNIVERSITY

发明人： DIETZSCHOLD, Bernhard ,  FABER, Marie Luise ,  FABER, Milosz ,  SCHNELL, Matthias

IPC分类号： C12N7/00 ,  C12N15/00 ,  C07K14/00 ,  A61K39/205

CPC分类号： C12N7/00 ,  A61K35/13 ,  C07K14/005 ,  C12N2760/20121 ,  C12N2760/20122 ,  C12N2760/20161

公开(公告)号：EP2254992A1

公开(公告)日：2010-12-01

申请号：EP09718773.6

申请日：2009-03-09

申请人： Novartis Forschungsstiftung, Zweigniederlassung ,  University of Szeged

发明人： BALINT, Kamill ,  BOLDOGKOI, Zsolt ,  ROSKA, Botond

IPC分类号： C12N7/00 ,  G01N33/569

CPC分类号： C12N7/00 ,  C12N2710/16721 ,  C12N2710/16743 ,  C12N2760/20121 ,  C12N2760/20143 ,  G01N33/5058 ,  G01N33/542 ,  G01N33/56994

摘要： The application relates to an isolated transsynaptic virus expressing an exogenous fluorescent activity sensor. Preferably, the transsynaptic virus is a rhabdovirus, e.g. rabies virus, or a herpesvirus, preferably an alphaherpesvirus, e.g. pseudorabies virus. The fluorescent exogenous activity sensor used in the transsynaptic virus can be a fluorescent protein Ca
2+ sensor e.g. yellow cameleon, camgaroo, G-CaMP/Pericam. or TN-L15, preferably TN-L15, or a fluorescent protein voltage sensor, e.g. FIaSh, SPARC, or a VSP, preferably VSP1. The transsynaptic virus of the inventioncan be used in a method for analyzing the neural activity in local circuits. Moreover, the present application also relates to a kit for analyzing the neural activity in local circuits, said kit comprising a transsynaptic virus of the invention.

公开(公告)号：EP2585598A4

公开(公告)日：2014-04-16

申请号：EP11798901

申请日：2011-06-23

申请人： US GOV HEALTH & HUMAN SERV

发明人： WU XIANFU ,  RUPPRECHT CHARLES E ,  KUZMIN IVAN V

IPC分类号： C12N15/47 ,  A61K39/205 ,  C12N15/63

CPC分类号： A61K39/205 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/70 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2760/20121 ,  C12N2760/20122 ,  C12N2760/20134 ,  C12N2760/20143 ,  C12N2760/20152 ,  C12N2800/50

公开(公告)号：EP1768697A4

公开(公告)日：2008-03-05

申请号：EP05790962

申请日：2005-07-12

申请人： UNIV JEFFERSON

发明人： DIETZSCHOLD BERNHARD ,  FABER MARIE LUISE ,  FABER MILOSZ ,  SCHNELL MATTHIAS

IPC分类号： C12N7/00 ,  A61K39/205 ,  C07K14/00 ,  C12N15/00

CPC分类号： C12N7/00 ,  A61K35/13 ,  C07K14/005 ,  C12N2760/20121 ,  C12N2760/20122 ,  C12N2760/20161

摘要： Recombinant rabies viruses in which the arginine residue of the glycoprotein (G) at amino acid position 333 is exchanged, renders these viruses nonpathogenic for immunocompetent mammals regardless of the route of infection. Some of these recombinant rabies viruses after several serial virus passages in newborn mice can become pathogenic for adult mice. The reversion to the pathogenic phenotype is associated with a thymidine to adenosine mutation (TôA) at position 639 of the G gene, which results in an asparagine to lysine exchange at position 194 of G. The codon at position 637-639 was changed by site directed mutagenesis to replace asparagine at position 194 by an amino acid that minimized the possibility for an AsnôLys exchange at amino acid position 194 of G and prevents reversion to a pathogenic form of the virus.

公开(公告)号：EP2585598A2

公开(公告)日：2013-05-01

申请号：EP11798901.2

申请日：2011-06-23

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Services

发明人： WU, Xianfu ,  RUPPRECHT, Charles, E. ,  KUZMIN, Ivan, V.

IPC分类号： C12N15/47 ,  C12N15/63 ,  A61K39/205

CPC分类号： A61K39/205 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/70 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2760/20121 ,  C12N2760/20122 ,  C12N2760/20134 ,  C12N2760/20143 ,  C12N2760/20152 ,  C12N2800/50

摘要： Described herein are recombinant rabies viruses encoding rabies virus glycoprotein and at least one heterologous glycoprotein from another lyssavirus, such as Mokola virus, Lagos bat virus and/or West Caucasian bat virus. In particular embodiments, the recombinant rabies virus includes two or three heterologous lyssavirus glycoproteins. The disclosed recombinant rabies viruses can be used as pan-lyssavirus vaccines to provide protection against lyssaviruses that cause rabies.

公开(公告)号：EP1768697A2

公开(公告)日：2007-04-04

申请号：EP05790962.4

申请日：2005-07-12

申请人： THOMAS JEFFERSON UNIVERSITY

发明人： DIETZSCHOLD, Bernhard ,  FABER, Marie Luise ,  FABER, Milosz ,  PAK, Koon Yan ,  MATTIS, Jeffrey, A.

IPC分类号： A61K39/205

CPC分类号： C12N7/00 ,  A61K35/13 ,  C07K14/005 ,  C12N2760/20121 ,  C12N2760/20122 ,  C12N2760/20161

摘要： Recombinant rabies viruses in which the arginine residue of the glycoprotein (G) at amino acid position 333 is exchanged, renders these viruses nonpathogenic for immunocompetent mammals regardless of the route of infection. Some of these recombinant rabies viruses after several serial virus passages in newborn mice can become pathogenic for adult mice. The reversion to the pathogenic phenotype is associated with a thymidine to adenosine mutation (TôA) at position 639 of the G gene, which results in an asparagine to lysine exchange at position 194 of G. The codon at position 637-639 was changed by site directed mutagenesis to replace asparagine at position 194 by an amino acid that minimized the possibility for an AsnôLys exchange at amino acid position 194 of G and prevents reversion to a pathogenic form of the virus.