公开(公告)号：EP4353824A1

公开(公告)日：2024-04-17

申请号：EP22201471.4

申请日：2022-10-13

申请人： Universidade de Évora

发明人： REIS VARANDA, CARLA MARISA ,  DE QUEIROZ MATERATSKI, PATRICK JOSÉ ,  FERNANDES FÉLIX, MARIA DO ROSÁRIO ,  MURTEIRA RICO DA COSTA CAMPOS, MARIA DOROTEIA ,  CARDOSO PATANITA, MARIANA ,  BARRETO ALBUQUERQUE, ANDRÉ FILIPE ,  AMARO RIBEIRO, JOANA

IPC分类号： C12N15/82 ,  C07K14/08

CPC分类号： C12N15/8203 ,  C12N15/8218 ,  C12N15/8283 ,  C12N15/86 ,  C07K14/005 ,  C12N2760/1202220130101 ,  C12N2770/3804320130101 ,  C12N2770/3803120130101 ,  C12N2760/1203120130101

摘要： The invention relates to a viral vector for conferring protection of plants against Tomato spotted wilt virus (TSWV), a virus that causes a devastating disease in tomato plants. The virus-based vector was modified to carry part of the TSWV non-structural protein NSm gene, conferring protection against TSWV in plants. The invention also relates to a process for silencing a target gene in a plant comprising the steps of i) obtaining a construct according to the previous claim; ii) introducing the construct inside the plant, preferably by using viral suspensions; inoculating into plants, preferably by spraying plants, even more preferably by spraying plants with a compressor set comprising an airbrush; iii) activating the plant's specific defence mechanism, consequently silencing the target gene. The present invention also discloses a composition for plant disease management and/or plant immunization.

公开(公告)号：EP4353740A1

公开(公告)日：2024-04-17

申请号：EP22201482.1

申请日：2022-10-13

申请人： Universidade de Évora

发明人： DE QUEIROZ MATERATSKI, PATRICK JOSÉ ,  REIS VARANDA, CARLA MARISA ,  MURTEIRA RICO DA COSTA CAMPOS, MARIA DOROTEIA ,  CARDOSO PATANITA, MARIANA ,  BARRETO ALBUQUERQUE, ANDRÉ FILIPE ,  AMARO RIBEIRO, JOANA ,  FERNANDES FÉLIX, MARIA DO ROSÁRIO

IPC分类号： C07K14/37 ,  C12N15/82

CPC分类号： C07K14/37 ,  C12N15/8218 ,  C12N15/8282 ,  C12N15/86 ,  C12N2770/3804320130101

摘要： The present invention discloses an isolated sequence having at least 95% homology with SEQ ID No 1, preferably at least 98% homology, even more preferably having at least 99% homology, the isolated sequence comprising at least one point mutation selected from: adenine to thymine at nucleotide 3200, cytosine to guanine at nucleotide 3361, guanine to cytosine at nucleotide 3365 and/or guanine to thymine at nucleotide 3368. The invention also relates to a process for silencing a target gene in a plant comprising the steps of i) obtaining a construct according to the previous claim; ii) introducing the construct inside the plant, preferably by using viral suspensions; inoculating into plants, preferably by spraying plants, even more preferably by spraying plants with a compressor set comprising an airbrush; iii) activating the plant's specific defence mechanism, consequently silencing the target gene. The present invention also discloses a composition for plant disease management and/or plant immunization.