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    METHOD FOR DETERMINING SNP GENOTYPE
    5.
    发明公开
    METHOD FOR DETERMINING SNP GENOTYPE 审中-公开
    VERFAHREN ZUR BESTIMMUNG EINES SNP-GENOTYPS

    公开(公告)号：EP3027772A4

    公开(公告)日：2017-04-05

    申请号：EP14831476

    申请日：2014-07-30

    申请人： SEEGENE INC

    发明人： CHUN JONG YOON ,  LEE YOUNG JO

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6883 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2527/107 ,  C12Q2533/101 ,  C12Q2561/109 ,  C12Q2565/1015

    摘要： The present invention is generally drawn to a novel method for determining a SNP (single nucleotide polymorphism) genotype using a PTO-SNV (Probing and Tagging Oligonucleotide for Single Nucleotide Variation). The present invention provides novel protocols for SNP genotyping in which only one allele-specific oligonucleotide permits in a SNP genotyping reaction to determine whether a target nucleic acid sequence to be analyzed is homozygous or heterozygous for the SNP allele of interest or has no SNP allele of interest.

    摘要翻译： 本发明一般涉及使用PTO-SNV（用于单核苷酸变异的探针和标记寡核苷酸）测定SNP（单核苷酸多态性）基因型的新方法。 本发明提供用于SNP基因分型的新方案，其中只有一个等位基因特异性寡核苷酸允许在SNP基因分型反应中确定待分析的靶核酸序列是否为感兴趣的SNP等位基因是纯合的或杂合的，或者没有SNP等位基因 利益。

    DETECTION OF DNA METHYLATION USING COMBINED NUCLEASE LIGATION REACTIONS
    6.
    发明公开
    DETECTION OF DNA METHYLATION USING COMBINED NUCLEASE LIGATION REACTIONS 审中-公开
    NACHWEIS VON DNA-METHYLIERUNG UNTER VERWENDUNG KOMBINIERTER NUKLEASELIGATIONSREAKINGEN

    公开(公告)号：EP3126523A2

    公开(公告)日：2017-02-08

    申请号：EP15772834.6

    申请日：2015-03-31

    申请人： Cornell University

    发明人： BARANY, Francis

    IPC分类号： C12Q1/68 ,  C40B20/00 ,  C40B30/04 ,  C40B40/06

    CPC分类号： C12Q1/683 ,  C12Q2521/331 ,  C12Q2525/301 ,  C12Q2561/109 ,  C12Q2533/107

    摘要： The present invention is directed to methods for identifying the presence of one or more methylated or unmethylated target nucleotide sequences in a sample that involve coupled methylation sensitive restriction enzyme digestion-ligation and/or extension processes. In some embodiments, the ligation and primary extension products formed in the reaction processes of the present invention are subsequently amplified using a polymerase chain reaction. The ligation products or primary extension products are detected, and the presence of one or more methylated or unmethylated target nucleotide sequences in the sample is identified based on the detection.

    摘要翻译： 本发明涉及用于鉴定样品中一种或多种甲基化或非甲基化靶核苷酸序列的存在的方法，其涉及偶联的甲基化敏感性限制酶消化连接和/或延伸过程。 在一些实施方案中，随后使用聚合酶链式反应扩增在本发明的反应过程中形成的连接和初级延伸产物。 检测连接产物或初级延伸产物，并且基于检测鉴定样品中一个或多个甲基化或未甲基化的靶核苷酸序列的存在。

    Chimeric primers with hairpin conformations and methods of using same
    8.
    发明公开
    Chimeric primers with hairpin conformations and methods of using same 有权
    ChimärePrimer mit Haarnadel-Konformationen und Verfahren zur Verwendung davon

    公开(公告)号：EP2789689A1

    公开(公告)日：2014-10-15

    申请号：EP14168543.8

    申请日：2010-06-29

    申请人： Luminex Corporation

    发明人： Whitman, Douglas F. ,  Zhang, Hongwei

    IPC分类号： C12N15/11 ,  C07H21/00 ,  C12Q1/68

    CPC分类号： C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6876 ,  C12Q2525/186 ,  C12Q2525/301 ,  C12Q2561/109 ,  C12Q2563/149

    摘要： Methods and compositions for nucleic acid amplification, detection, and genotyping techniques are disclosed. In one embodiment, a nucleic acid molecule having a target-specific primer sequence; an anti-tag sequence 5' of the target-specific primer sequence; a tag sequence 5 'of the anti-tag sequence; and a blocker between the anti-tag sequence and the tag sequence is disclosed. Compositions containing such a nucleic acid molecule and methods of using such a nucleic acid molecule are also disclosed.

    摘要翻译： 公开了用于核酸扩增，检测和基因分型技术的方法和组合物。 在一个实施方案中，具有靶特异性引物序列的核酸分子; 靶标特异性引物序列的抗标签序列5'; 抗标签序列的标签序列5'; 并且公开了抗标签序列和标签序列之间的阻断剂。 还公开了含有这种核酸分子的组合物和使用这种核酸分子的方法。

    METHYLATION ASSAY
    9.
    发明公开
    METHYLATION ASSAY 有权
    甲基化分析

    公开(公告)号：EP2640858A2

    公开(公告)日：2013-09-25

    申请号：EP11842202.1

    申请日：2011-11-02

    申请人： Exact Sciences Corporation

    发明人： ZOU, Hongzhi ,  LIDGARD, Graham, P. ,  DOMANICO, Michael, J. ,  ALLAWI, Hatim

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6886 ,  C12Q1/6827 ,  C12Q2600/154 ,  C12Q2521/301 ,  C12Q2523/125 ,  C12Q2561/109

    摘要： A method for detecting a methylated genomic locus is provided. In certain embodiments, the method comprises: a) treating a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an agent that modifies cytosine to uracil to produce a treated nucleic acid; b) amplifying a product from the treated nucleic acid using a first primer and a second primer, wherein the first primer hybridizes to a site in the locus that contain methylcytosines and the amplifying preferentially amplifies the methylated copies of the genomic locus, to produce an amplified sample; and c) detecting the presence of amplified methylated copies of the genomic locus in the amplified sample using a flap assay that employs an invasive oligonucleotide having a 3' terminal G or C nucleotide that corresponds to a site of methylation in the genomic locus.