QUALITY EVALUATION METHOD FOR SPHEROID INCLUDING HEPATOCYTES

    公开(公告)号:EP4428812A1

    公开(公告)日:2024-09-11

    申请号:EP24158147.9

    申请日:2024-02-16

    IPC分类号: G06T7/00

    摘要: According to this evaluation method, first, spheroids obtained by three-dimensional culture of multiple kinds of liver-derived cells are imaged by optical coherence photography (image acquisition step), a localization region is extracted from the photographic image (region extraction step), the localization region is analyzed (analysis step), and the condition of the spheroid is evaluated (evaluation step). The analysis step includes a first calculation step (S41) of calculating the area of the entire spheroid in the photographic image, a second calculation step (S42) of calculating the area of the localization region, a third calculation step (S43) of calculating the ratio of the localization region on the basis of the two areas, and a fourth calculation step (S44) of calculating an evaluation parameter on the basis of the ratio. With the use of this evaluation parameter, there is provided an evaluation method that makes it possible to noninvasively observe a localization region of a spheroid used in a bio 3D printer and evaluate the condition of the spheroid without processing cells by staining or the like.