摘要:
The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder, a pump and a plurality of valves configured to at least partially control fluid flow through a fluid circuitry and a separation column positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.
摘要:
A flow enhanced method and system for flow non-linear magnetophoresis (F-NLM) is described. By tuning an external field frequency and the flow rate the migration velocities of different bead types may be caused to differ by several orders of magnitude over an extended range of frequencies to allow for separation of particles. Use of such efficiency in separation in bio-separation and similar assays is described.
摘要:
A filter device for filtering a contaminated fluid containing particulates comprises: a filter tank (11) into which the contaminated fluid is introduced; a magnetic ball filter medium (33) which is formed of an aggregate of a large number of spherical metal balls (35) of a magnetic material and is held in the filter tank (11) in a manner such that the metal balls (35) are movable relatively to one another; a magnet (45) relatively movable with respect to the magnetic ball filter medium (33) between a first position (A) on the lower side and a second position (B) on the upper side and configured to apply a magnetic field to the metal balls (35), thereby causing the metal balls (35) magnetically to attract one another and be fixed to one another when in the first position (A), and to cancel the magnetic attraction between the metal balls (35) when in the second position (B); a dirty chamber (15) which is defined under the magnetic ball filter medium (33) and into which the contaminated fluid is introduced; and a clean chamber (16) which is defined over the magnetic ball filter medium (33), the clean chamber (16) containing a cleaned fluid filtered through the magnetic ball filter medium (33), a magnet guide (41) in the form of bottomed container is inserted in a magnet storage portion (40) in the filter tank (11), the magnetic ball filter medium (33) is disposed both sides of the magnet guide (41), the magnet guide (41) contains the magnet (45) which is movable along the magnet guide (41) between the first position (A) on the lower side and the second position (B) on the upper side, holding means (50, 51, 52) for relatively moving the magnet (45) between the first position (A) and the second position (B) along the magnet guide (41) in the filter tank (11), the holding means (50, 51, 52) moving the magnet (45) to the first position (A) during filtering operation and to the second position (B) so that the metal balls (35) are movable relatively to one another when the metal balls (35) are cleaned.