SULFONATED CELLULOSE MICROFIBERS AND METHOD FOR PRODUCING SAME

    公开(公告)号:EP4029983A1

    公开(公告)日:2022-07-20

    申请号:EP20862987.3

    申请日:2020-09-10

    Abstract: [Object] The present invention provides a sulfonated fine cellulose fiber having excellent viscosity and the like, as well as a sulfonated fine cellulose fiber production method capable of efficiently producing the sulfonated fine cellulose fibers.
    [Method for Achieving the Object] A fine cellulose fiber obtained by fibrillating a cellulose fiber wherein a part of the hydroxyl groups of the fine cellulose fiber is substituted with sulfo groups, and an introduction amount of sulfur attributable to the sulfo groups is higher than 0.42 mmol/g. The fine cellulose fiber comprises a plurality of unit fibers and has an average fiber width of 30 nm or less. A dispersion liquid in which the fine cellulose fiber is dispersed in a water-soluble solvent at a solid concentration of 0.5% by mass has a viscosity of 5,000 mPa·s or more at 25°C. This makes it possible to obtain the desired viscosity when the fine cellulose fibers are dispersed in a dispersion liquid.

    Cellulose sulfate salt having anti-coagulating action and process for preparing same
    6.
    发明公开
    Cellulose sulfate salt having anti-coagulating action and process for preparing same 失效
    纤维素硫酸酯具有抗凝血活性和其制备方法的盐。

    公开(公告)号:EP0053473A1

    公开(公告)日:1982-06-09

    申请号:EP81305569.6

    申请日:1981-11-25

    CPC classification number: C08B5/14

    Abstract: A cellulose sulfate salt having a heparinic action is provided, which satisfies requirements of 0.8 ≦ "F" ≦ 2.6, "f 2 " ≧ "f 3 " and "f 2 " ≧ "f 6 " wherein "f 2 ", "f 3 " and "f 6 " stand for probability of substitution of atoms H in the OH groups bonded to the C 2 , C 3 and C 6 positions of the glucopyranose ring unit by sulfuric acid radicals, respectively, and "F" stands for the total substitution degree which is the sum of "f 2 ", "f 3 " and "f s ". The cellulose sulfate salt is prepared by reacting a cellulose having a crystal form I with an S0 3 /amine or S0 3 /amide complex in an amount of 2 to 4 mole equivalents per glucose unit of the cellulose at -10°C to 40°C to form a gelatinous product; adding water to the gelatinous product in an amount 0.1 to 5 times the amount of the starting cellulose used for the reaction; treating the mixture at a high temperature to relatively reduce the values of "«f 6 " and "f 3 " among the values of "f 2 ", "f 3 " and "f 6 "; neutralizing, precipitating and drying the so obtained crude cellulose sulfate; dissolving the crude cellulose sulfate in water again; treating the solution with an adsorbent; and subjecting the treated cellulose sulfate to precipitation, drying, re-dissolution, dialysis, precipitation and then drying.

    Process for the preparation of magnetic sulfated cellulose particles, magnetic sulfated cellulose particles and their use
    7.
    发明公开
    Process for the preparation of magnetic sulfated cellulose particles, magnetic sulfated cellulose particles and their use 审中-公开
    一种用于生产磁性硫酸化纤维素的,硫酸化的磁性纤维素及其应用过程

    公开(公告)号:EP2966093A1

    公开(公告)日:2016-01-13

    申请号:EP14175925.8

    申请日:2014-07-07

    CPC classification number: C08B5/14 C12N7/00 C12N2760/16151

    Abstract: In a first aspect, the present invention relates to a process for the preparation of magnetic sulfated cellulose particles. In particular, the present invention relates to the preparation of magnetic sulfated cellulose particles under specific reaction conditions allowing providing said magnetic sulfated cellulose particles being particularly useful in further process steps including enrichment of proteinaceous compositions. In another aspect, the present invention relates to the magnetic sulfated cellulose particles itself as well as its use for isolation of proteinaceous compositions including composition of virus proteins and/or non-virus proteins as well as whole virus and for enrichment steps in in vitro diagnostic methods. Finally, the present invention provides a method for isolating whole virus or virus proteins as well as isolating heparin binding molecules comprising the step of affinity purification using the magnetic sulfated cellulose particles according to the present invention. The present invention relates further to a bioreactor system comprising a bioreactor and a magnetic separation unit useful for separating magnetic sulfated cellulose particles according to the present invention.

    Abstract translation: 在第一方面,本发明涉及用于磁性硫酸化纤维素颗粒的制备方法。 特别地,本发明涉及磁性硫酸化纤维素颗粒的特定的反应条件允许提供所述磁性硫酸化纤维素颗粒是在进一步处理中特别有用下制备步骤蛋白质的组合物,包括富集。 在另一个方面中,本发明涉及磁性硫酸化纤维素颗粒本身以及其对蛋白质组合物,包括病毒蛋白和/或非病毒蛋白的组合物的隔离使用以及全病毒和用于富集步骤的体外诊断 方法。 最后,本发明提供了用于分离完整病毒或病毒蛋白以及分离的肝素结合分子,其包括使用磁性硫酸化纤维素颗粒gemäß到本发明的亲和纯化的步骤的方法。 本发明还涉及一种生物反应器系统,其包括生物反应器和磁分离单元,用于分离磁性粒子硫酸纤维素gemäß对本发明是有用的。

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