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公开(公告)号：JP2010215657A
公开(公告)日：2010-09-30
申请号：JP2010134726
申请日：2010-06-14
申请人： Amgen , アムジエン・インコーポレーテツド
IPC分类号： A61K38/21 , C07K1/113 , A61K31/00 , A61K38/00 , A61K38/19 , A61K47/30 , A61K47/34 , A61K47/36 , A61K47/48 , A61P1/16 , A61P3/06 , A61P7/00 , A61P7/06 , A61P9/10 , A61P25/00 , A61P31/12 , A61P35/00 , A61P37/00 , A61P37/04 , C07K20060101 , C07K1/06 , C07K1/107 , C07K1/13 , C07K14/52 , C07K14/53 , C07K14/535 , C07K14/555 , C07K14/56 , C07K17/08
CPC分类号： A61K47/4823 , A61K38/00 , A61K47/60 , C07K14/535 , C07K14/56
摘要： PROBLEM TO BE SOLVED: To provide a composition containing uniform protein by selectively bonding a water-soluble polymer to the N-terminal of the protein. SOLUTION: This invention provides a method of producing a composition containing substantially uniform and stable protein of monopolymer/protein combination by selectively modifying α-amino acid at the N-terminal of the protein with a water-soluble polymer such as dextran, poly(N-vinyl pyrrolidone), polyethylene glycol using reductive alkylation using a reducing agent such as sodium borohydride and sodium cyanoborohydride. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译： 待解决的问题：通过选择性地将水溶性聚合物结合到蛋白质的N末端来提供含有均匀蛋白质的组合物。 解决方案：本发明提供了一种制备含有基本均匀稳定的单体/蛋白质组合蛋白质的组合物的方法，其通过用水溶性聚合物如葡聚糖选择性地修饰蛋白质N末端的α-氨基酸， 聚（N-乙烯基吡咯烷酮），使用还原剂例如硼氢化钠和氰基硼氢化钠进行还原烷基化的聚乙二醇。 版权所有（C）2010，JPO＆INPIT
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公开(公告)号：JP2006077021A
公开(公告)日：2006-03-23
申请号：JP2005286188
申请日：2005-09-30
申请人： Amgen , アムジエン・インコーポレーテツド
IPC分类号： A61K38/21 , C07K1/02 , A61K31/00 , A61K38/00 , A61K38/19 , A61K47/30 , A61K47/48 , A61P1/16 , A61P3/06 , A61P7/00 , A61P7/06 , A61P9/10 , A61P25/00 , A61P31/12 , A61P35/00 , A61P37/00 , A61P37/04 , C07K20060101 , C07K1/06 , C07K1/107 , C07K1/113 , C07K1/13 , C07K14/52 , C07K14/53 , C07K14/535 , C07K14/555 , C07K14/56 , C07K17/08 , C12N15/09
CPC分类号： A61K47/4823 , A61K38/00 , A61K47/60 , C07K14/535 , C07K14/56
摘要： PROBLEM TO BE SOLVED: To produce a homogeneous protein-containing composition by selectively bonding a water-soluble polymer to the N terminals of a protein. SOLUTION: What is provided is a method for producing a stable protein-containing composition containing a substantially homogeneous monopolymer/protein conjugate by selectively modifying the α-amino acids at the N-terminals of a protein with a water-soluble polymer such as dextran, poly(N-vinylpyrrolidone), or polyethylene glycol by using reductive alkylation using a reducing agent such as sodium borohydride or sodium cyanoborohydride. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译： 待解决的问题：通过选择性地将水溶性聚合物结合到蛋白质的N末端来制备均匀的含蛋白质的组合物。 解决方案：提供了通过用水溶性聚合物选择性地修饰蛋白质N末端的α-氨基酸来生产含有基本均匀的单体/蛋白质缀合物的稳定的含蛋白质组合物的方法，例如 作为葡聚糖，聚（N-乙烯基吡咯烷酮）或聚乙二醇，使用还原剂如硼氢化钠或氰基硼氢化钠进行还原烷基化。 版权所有（C）2006，JPO＆NCIPI
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公开(公告)号：JP2006045243A
公开(公告)日：2006-02-16
申请号：JP2005286189
申请日：2005-09-30
申请人： Amgen , アムジエン・インコーポレーテツド
IPC分类号： A61K38/00 , A61K38/21 , A61K31/00 , A61K38/19 , A61K47/30 , A61K47/48 , A61P1/16 , A61P3/06 , A61P7/00 , A61P7/06 , A61P9/10 , A61P25/00 , A61P31/12 , A61P35/00 , A61P37/00 , A61P37/04 , C07K20060101 , C07K1/06 , C07K1/107 , C07K1/113 , C07K1/13 , C07K14/52 , C07K14/53 , C07K14/535 , C07K14/555 , C07K14/56 , C07K17/08
CPC分类号： A61K47/4823 , A61K38/00 , A61K47/60 , C07K14/535 , C07K14/56
摘要： PROBLEM TO BE SOLVED: To produce a composition containing a homogeneous protein by selectively binding a water-soluble polymer to the N-terminus of the protein. SOLUTION: The method for producing the stable composition containing the protein of a substantially homogeneous monopolymer/protein conjugate, obtained by selectively modifying the α-amino acid at the N-terminus of the protein with a polyethylene glycol by using a reductive alkylation using a reducing agent such as sodium borohydride and sodium cyanoborohydride. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译： 待解决的问题：通过选择性地将水溶性聚合物结合到蛋白质的N末端来制备含有均匀蛋白质的组合物。 解决方案：制备含有基本上均匀的单体/蛋白质缀合物的蛋白质的稳定组合物的方法，其通过使用还原性烷基化选择性地用聚乙二醇改性蛋白质N-末端的α-氨基酸而获得 使用还原剂如硼氢化钠和氰基硼氢化钠。 版权所有（C）2006，JPO＆NCIPI
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公开(公告)号：JP2003327600A
公开(公告)日：2003-11-19
申请号：JP2003106520
申请日：2003-04-10
申请人： Amgen , アムジエン・インコーポレーテツド
IPC分类号： A61K38/21 , A61K31/00 , A61K38/00 , A61K38/19 , A61K47/30 , A61K47/48 , A61P1/16 , A61P3/06 , A61P7/00 , A61P7/06 , A61P9/10 , A61P25/00 , A61P31/12 , A61P35/00 , A61P37/00 , A61P37/04 , C07K20060101 , C07K1/06 , C07K1/107 , C07K1/113 , C07K1/13 , C07K14/52 , C07K14/53 , C07K14/535 , C07K14/555 , C07K14/56 , C07K17/08
CPC分类号： A61K47/4823 , A61K38/00 , A61K47/60 , C07K14/535 , C07K14/56
摘要： PROBLEM TO BE SOLVED: To produce a composition containing a homogeneous protein by selectively bonding a water-soluble polymer to the N-terminal of the protein. SOLUTION: The method for producing the composition containing the substantially homogeneous stable protein of a monopolymer/protein conjugate involves selectively modifying an α-amino acid at the N-terminal of the protein with a polyethylene glycol by using a reductive alkylation reaction using a reducing agent such as sodium borohydride and sodium cyanoborohydride. COPYRIGHT: (C)2004,JPO
摘要翻译： 待解决的问题：通过选择性地将水溶性聚合物结合到蛋白质的N末端来制备含有均匀蛋白质的组合物。 解决方案：含有单体/蛋白质结合物的基本均匀的稳定蛋白质的组合物的制备方法涉及通过使用还原性烷基化反应使用聚乙二醇选择性地修饰蛋白质N-末端的α-氨基酸，使用 还原剂如硼氢化钠和氰基硼氢化钠。 版权所有（C）2004，JPO
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公开(公告)号：JP2003155299A
公开(公告)日：2003-05-27
申请号：JP2002288746
申请日：2002-10-01
申请人： AMGEN
IPC分类号： A61K38/21 , A61K31/00 , A61K38/00 , A61K38/19 , A61K47/30 , A61K47/48 , A61P1/16 , A61P3/06 , A61P7/00 , A61P7/06 , A61P9/10 , A61P25/00 , A61P31/12 , A61P35/00 , A61P37/00 , A61P37/04 , C07K20060101 , C07K1/06 , C07K1/107 , C07K1/113 , C07K1/13 , C07K14/52 , C07K14/53 , C07K14/535 , C07K14/555 , C07K14/56 , C07K17/08
摘要： PROBLEM TO BE SOLVED: To selectively bind a water-soluble polymer to the N-terminal of a protein to produce a homogeneous protein-containing composition. SOLUTION: The method for producing a stable protein-containing composition substantially homogeneously containing a monopolymer/protein combination comprises selectively modifying an α-amino acid at the N-terminal of the protein with a water-soluble polymer such as dextran, poly(N-vinylpyrrolidone) or a polyethylene glycol in a reducing alkylation reaction using a reducing agent such as sodium borohydride, or sodium cyanoborohydride.
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公开(公告)号：JPH11310600A
公开(公告)日：1999-11-09
申请号：JP7695999
申请日：1999-03-19
申请人： AMGEN
IPC分类号： A61K38/21 , A61K31/00 , A61K38/00 , A61K38/19 , A61K47/30 , A61K47/48 , A61P1/16 , A61P3/06 , A61P7/00 , A61P7/06 , A61P9/10 , A61P25/00 , A61P31/12 , A61P35/00 , A61P37/00 , A61P37/04 , C07K20060101 , C07K1/06 , C07K1/107 , C07K1/113 , C07K1/13 , C07K14/52 , C07K14/53 , C07K14/535 , C07K14/555 , C07K14/56 , C07K17/08
摘要： PROBLEM TO BE SOLVED: To obtain a protein composition containing a cooperative interferon, etc., by binding a reactive aldehyde group-containing water-soluble polymer to an α-amino group such as a protein or the like under a reductive alkylation reaction condition and then separating the reaction product. SOLUTION: In this method for binding a water-soluble polymer containing a single reactive aldehyde group to a protein or its analog, the protein part is reacted with a water-soluble polymer part of a poly(N-vinylpyrrolidone), a polyethylene glycol, a polyvinyl alcohol or the like under reductive alkylation conditions using sodium boron hydride or the like at an acidic pH sufficient to selectively activate the α-amino group of the amino end of the protein part to selectively bond the water-soluble polymer to the α-amino group and the reaction product is separated from the unreacted part to give the objective chemically modified protein composition such as an N-terminal chemically modified G-CSF composition, a chemically modified cooperative interferon, etc.
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