公开(公告)号：JP2011083342A

公开(公告)日：2011-04-28

申请号：JP2009236777

申请日：2009-10-14

申请人： Kowa Co ,  興和株式会社

发明人： HIRONO TAIRYO

IPC分类号： A61B3/117 ,  A61B3/10 ,  G01N21/03 ,  G01N21/39 ,  G01N21/49 ,  G01N33/483 ,  G01N33/68

CPC分类号： A61B3/10 ,  G01N21/39 ,  G01N21/51 ,  G01N2021/399

摘要： PROBLEM TO BE SOLVED: To provide a molecular composition measuring method and device capable of measuring the molecular composition or concentration of a sample or of protein in the anterior chamber of a human eye with high reliability, in non-contact and non-invasive manner. SOLUTION: Laser beam fluxes of different wavelengths are emitted from a wavelength variable laser light source 10 into the anterior chamber 21 of the human eye including molecules of protein of albumin and globulin. The scattering light from the protein molecules is received by a detector 8, and an autocorrelation function of the scattering light signal is found by a correlator 9. A computing device 11 computes the concentration of albumin and of globulin or the ratio between the concentrations based on the autocorrelation function of the scattering light signals of albumin and globulin irradiated with the laser beam fluxes of different wavelengths respectively. With this structure, the concentrations of albumin and globulin, or the ratio between the concentrations can be reliably measured in non-contact and non-invasive manner. COPYRIGHT: (C)2011,JPO&INPIT

摘要翻译： 要解决的问题：为了提供能够以高可靠性测量人眼前房中的样品或蛋白质的分子组成或浓度的非接触和非接触的分子组成测量方法和装置， 侵入式。 解决方案：不同波长的激光束通量从波长可变激光光源10发射到人眼前房21，包括白蛋白和球蛋白的蛋白质分子。 来自蛋白质分子的散射光被检测器8接收，相关器9发现散射光信号的自相关函数。计算装置11计算白蛋白和球蛋白的浓度或基于 分别用不同波长的激光束照射的白蛋白和球蛋白的散射光信号的自相关函数。 通过这种结构，白蛋白和球蛋白的浓度或浓度之间的比例可以非接触和非侵入的方式可靠地测量。 版权所有（C）2011，JPO＆INPIT

公开(公告)号：JP2007315754A

公开(公告)日：2007-12-06

申请号：JP2006142215

申请日：2006-05-23

申请人： Kowa Co ,  Univ Of Electro-Communications ,  国立大学法人 電気通信大学 ,  興和株式会社

发明人： YAMADA YUKIO ,  TSUNODA NAOTO ,  HIRONO TAIRYO

IPC分类号： G01N35/08 ,  G01N37/00

CPC分类号： B01L3/502776 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/163 ,  G01N33/4905 ,  G01N2035/00544

摘要： PROBLEM TO BE SOLVED: To accurately apply an object substance onto a fine area, on the inner wall surface of a channel having a fine section.
SOLUTION: First, an affinity substance is applied onto the first domain (hatched region shown by the code M
1 in Figure (a), which is a domain along each of directions K
1 , K
2 , K
3 crossing the longitudinal direction J of the passage B
3 ) on the inner wall of the passage B
3 . Then, a first solution D
1 , containing the object substance and a second solution D
2 that does not contain the object substance are allowed to flow in a laminar flow state in the passage B
3 . As a result, the object substance can adhere accurately to the second region (namely, a part shown by the code M
2 in Figure (b)), in contact with the first solution D
1 in the first region M
1 .
COPYRIGHT: (C)2008,JPO&INPIT

摘要翻译： 要解决的问题：在精细部分的通道的内壁表面上精确地将物体施加到细小区域上。 解决方案：首先，将亲和性物质施加到第一区域（图（a）中由代码M 1 所示的阴影线区域），该区域沿着每个方向K 在通道的内壁上与通道B的长度方向J交叉的1 ，K 2 ，K 3 ） 乙 3 。 然后，使含有不含有物质的目标物质的第一溶液D 1 和不含有物质的第二溶液D 2 在 通道B 3 。 结果，物体可以精确地粘附到与第一溶液D 1接触的第二区域（即，图（b）中的代码M 2 所示的部分） 在第一区域M 1 。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2005147857A

公开(公告)日：2005-06-09

申请号：JP2003386107

申请日：2003-11-17

申请人： Kowa Co ,  興和株式会社

发明人： MITSUMOTO KOTARO ,  HIRONO TAIRYO

IPC分类号： G01N15/14

摘要： PROBLEM TO BE SOLVED: To provide an improved calibration method and calibration device for a suspended particle measuring instrument.
SOLUTION: In this calibration method for the particle measuring instrument, a rotation reflecting member is arranged in a particle-measuring position of the particle-measuring instrument, provided with a light-scattering part for irradiating particles with light and a measuring part for the scattered light and/or fluorescence by the particles, and measurement operation for the instrument is conducted to calibrate a measuring system of the instrument, based on the measured value therein. This calibration device for the instrument is provided with the rotatable reflection member, and a power part for rotating an attachment part for arranging the reflection member in the particle-measuring position of the particle measuring instrument, provided with the light-scattering part for irradiating the particles with the light and the measuring part for the scattered light and/or fluorescence by the particles, and the reflection member.
COPYRIGHT: (C)2005,JPO&NCIPI

摘要翻译： 要解决的问题：提供一种用于悬浮粒子测量仪器的改进的校准方法和校准装置。 解决方案：在该粒子测量装置的校准方法中，旋转反射部件布置在粒子测量装置的粒子测量位置，该粒子测量装置设有用于照射粒子的光散射部分和测量部分 对于颗粒的散射光和/或荧光，并且根据其中的测量值进行仪器的测量操作以校准仪器的测量系统。 该仪器的校准装置设置有可旋转反射构件，以及功率部件，用于旋转用于将反射构件布置在粒子测量仪器的粒子测量位置的安装部分，设置有用于照射的光散射部分 具有光的颗粒和用于颗粒的散射光和/或荧光的测量部分和反射构件。 版权所有（C）2005，JPO＆NCIPI

公开(公告)号：JP2010181253A

公开(公告)日：2010-08-19

申请号：JP2009024522

申请日：2009-02-05

申请人： Kowa Co ,  興和株式会社

发明人： HIRONO TAIRYO

IPC分类号： G01N33/49 ,  B01J19/00 ,  G01N37/00

CPC分类号： B01L3/502776 ,  B01L2300/0867 ,  B01L2400/0418 ,  B01L2400/0487

摘要： PROBLEM TO BE SOLVED: To provide a microchemical chip device capable of eliminating work to apply an agent to the wall surface of a microchannel.
SOLUTION: As shown in Fig.2(a), two laminar flows (i.e. a particle-containing solution A
1 and a buffer solution A
3 ) are supplied in a microchannel 20 in a microchemical chip device, and a gel including an agent A
2 is supplied to a location that contacts to the buffer solution A
3 . The reaction is started by the contact of a fine particle in the particle-containing solution A
1 to the gel including an agent A
2 . However, the reaction is not started by the separation due to the buffer solution A
3 in the state shown in Fig.2(a). When the buffer solution A
3 is stopped supplying in this condition, the particle-containing solution A
1 and the gel including an agent A
2 are contacted each other shown in Fig.2(c), which enables fixed point observation for the reaction of the particle containing and the agent. This device, which is not necessary to apply the wall surface of the microchannel 20 and is satisfied by supplying the gel A
2 including an agent from a supply channel 22, enables elimination of agent application.
COPYRIGHT: (C)2010,JPO&INPIT

摘要翻译： 要解决的问题：提供一种能够消除将试剂施加到微通道壁表面的工作的微化学芯片装置。 解决方案：如图2（a）所示，提供两个层流（即含有颗粒的溶液A 1 和缓冲溶液A 3） 在微量化学芯片装置中的微通道20中，并且将包含试剂A 2 的凝胶供给到与缓冲溶液A 3接触的位置。 通过使含有颗粒的溶液A 1 中的细颗粒与包含试剂A 2 的凝胶接触来开始反应。 但是，在图2（a）所示的状态下，由于缓冲液A 3 的分离，反应不能开始。 当在该条件下缓慢溶液A 3 停止供给时，含有颗粒的溶液A 1 和包含试剂A 2 的凝胶是 彼此接触，如图2（c）所示，这使得能够对含有颗粒和试剂的反应进行定点观察。 该装置不需要施加微通道20的壁面，并且通过从供给通道22供给包含试剂的凝胶A 2 可以消除试剂的应用。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2007315753A

公开(公告)日：2007-12-06

申请号：JP2006142214

申请日：2006-05-23

申请人： Kowa Co ,  Univ Of Electro-Communications ,  国立大学法人 電気通信大学 ,  興和株式会社

发明人： YAMADA YUKIO ,  TSUNODA NAOTO ,  HIRONO TAIRYO

IPC分类号： G01N37/00 ,  G01N33/49

CPC分类号： B01L3/502776 ,  B01L2200/0636 ,  B01L2200/143 ,  B01L2300/0816 ,  B01L2400/0487 ,  B01L2400/0622

摘要： PROBLEM TO BE SOLVED: To examine in detail the aggregation state of blood platelets by while the device is low in lost.
SOLUTION: Buffer solution D
1 and blood D
2 are allowed to flow in the layered state in a passage B
3 of a microchemical chip. An aggregate agent G for aggregating platelets in the blood is applied the wall surface on the side, whereupon the buffer solution D
1 flows. If the flow rate of the blood D
2 is heightened in this state, the laminar flow width W
2 of the blood can be widened, and the reaction between the aggregating agent G and the platelets can be analyzed in detail. Even when images or dynamic images are required to be taken, in order to compare the non-aggregation state with the aggregation state, by only photographing "a spot where the aggregating agent is applied (namely, a reaction part G)" is sufficient, and arrangement of two cameras or installation of a moving mechanism of the camera or the microchemical chip is not required to be installed, and the cost of the device can be reduced by this amount.
COPYRIGHT: (C)2008,JPO&INPIT

摘要翻译： 要解决的问题：在设备损失低的同时详细检查血小板的聚集状态。 解决方案：将缓冲溶液D 1 和血液D 2 在微量化学物质的通道B 3 中以分层状态流动 芯片。 将用于聚集血液中的血小板的聚集剂G施加在侧面上的壁表面，于是缓冲溶液D 1 流动。 如果在该状态下血液D 2 的流量升高，则血液的层流宽度W 2 可以变宽，聚集剂G 并且可以详细分析血小板。 即使需要拍摄图像或动态图像，为了将非聚集状态与聚合状态进行比较，仅通过拍摄“聚集剂被施加的点（即，反应部G）”就足够了， 并且不需要安装两个相机的布置或相机或微化学芯片的移动机构的安装，并且可以降低该装置的成本。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2012154815A

公开(公告)日：2012-08-16

申请号：JP2011014574

申请日：2011-01-26

申请人： Iwate Medical Univ ,  Kowa Co ,  学校法人 岩手医科大学 ,  興和株式会社

发明人： INADA KATSUYA ,  ENDO SHIGEATSU ,  HIRONO TAIRYO ,  ASANO TAKAHARU

IPC分类号： G01N33/579 ,  C12M1/34 ,  C12Q1/37

摘要： PROBLEM TO BE SOLVED: To improve the accuracy of a measurement when measuring the density of a biogenous physiologically active substance in a specimen by a stirring turbidimetric method, a light scattering method, or an AL-bound beads method, by suppressing aggregation and gelation that is not derived from the physiologically active substance but caused by stirring of liquid mixture.SOLUTION: When a protein aggregation derived from a reaction between AL and a biogenous physiologically active substance is detected by mixing AL and a specimen including the biogenous physiologically active substance while stirring the liquid mixture, a heat-treated predetermined protein is previously added to the liquid mixture so as to suppress the protein aggregation or gelation that is not derived from the reaction between the AL and the biogenous physiologically active substance in the liquid mixture.

摘要翻译： 要解决的问题：通过搅拌比浊法，光散射法或AL结合珠法测定样品中的生物生理活性物质的密度，通过抑制聚集来提高测量的精度 和不是衍生自生理活性物质但由液体混合物搅拌引起的凝胶化。 解决方案：当通过混合AL和包括生物生理活性物质的样品在搅拌液体混合物的同时检测来源于AL和生物生理活性物质之间的反应的蛋白质聚集时，预先加入经热处理的预定蛋白质 以抑制液体混合物中不与AL和生物生理活性物质的反应产生的蛋白质聚集或凝胶化。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2010185849A

公开(公告)日：2010-08-26

申请号：JP2009031852

申请日：2009-02-13

申请人： Kowa Co ,  興和株式会社

发明人： HIRONO TAIRYO

IPC分类号： G01N33/579 ,  G01N21/82 ,  G01N33/483

CPC分类号： G01N21/82 ,  G01N21/51 ,  G01N33/579

摘要： PROBLEM TO BE SOLVED: To provide an assay method capable of substantially shortening assay time when a physiologically active substance of biological origin such as an endotoxin or β-D-glucan is to be detected or its concentration is to be measured through the use of the reaction between the physiologically active substance and LAL and to provide an assay apparatus using the same. SOLUTION: The aggregation start time of a liquid mixture of an assay sample, an object of the detection of a physiologically active substance or the measurement of the concentration of the same, with LAL is detected. On the basis of this aggregation start time, the physiologically active substance is detected or the concentration thereof is measured. The liquid mixture of the assay sample with LAL is stirred with, for example, a magnetic stirrer to form gel particles, and the intensity of scattered light of laser light scattered by the gel particles is measured. A frequency distribution of fluctuations in the intensity of the scattered light is obtained. On the basis of the temporal change in the frequency distribution shape, the aggregation start time of the liquid mixture of the assay sample with LAL is detected. COPYRIGHT: (C)2010,JPO&INPIT

摘要翻译： 要解决的问题：提供一种能够在生物来源的生理活性物质例如内毒素或β-D-葡聚糖被检测或其浓度通过以下方法测量时基本缩短测定时间的测定方法： 使用生理活性物质和LAL之间的反应，并提供使用其的测定装置。 解决方案：检测测定样品的液体混合物的聚集开始时间，检测生理活性物质的对象或其浓度的测量与LAL的聚集开始时间。 基于该聚集开始时间，检测生理活性物质或其浓度。 将测定样品与LAL的液体混合物与例如磁力搅拌器一起搅拌以形成凝胶颗粒，并测量由凝胶颗粒散射的激光的散射光的强度。 获得散射光强度的波动的频率分布。 基于频率分布形状的时间变化，检测测定样品与LAL的液体混合物的聚集开始时间。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2008261737A

公开(公告)日：2008-10-30

申请号：JP2007104901

申请日：2007-04-12

申请人： Kowa Co ,  Univ Of Electro-Communications ,  国立大学法人 電気通信大学 ,  興和株式会社

发明人： YAMADA YUKIO ,  OKAWA SHINPEI ,  HIRONO TAIRYO

IPC分类号： G01N15/02 ,  G01N15/14

摘要： PROBLEM TO BE SOLVED: To measure a particle size accurately by a simple constitution.
SOLUTION: Particles A
1 , A
2 , A
3 flowing along a fine channel 2a are photographed by a camera 60, and the particle size is calculated from the image. If a particle is deviated to the front or rear side relative to a focal plane 60a of the camera 60 (refer to signs A
1 , A
3 ), the particle is photographed dimly and larger than the actual state, and therefore the calculated particle size is enlarged. In this invention, the position in the z-direction of each particle is determined, and deviation amounts Δz
1 , Δz
3 are calculated, to thereby correct the particle size. Consequently, the position in the z-direction of each particle can be determined only by a simple constitution having one camera, and the particle size can be measured accurately.
COPYRIGHT: (C)2009,JPO&INPIT

摘要翻译： 要解决的问题：通过简单的结构来准确地测量粒径。 解决方案：通过照相机60拍摄沿着细通道2a流动的颗粒A ，A 2 ，A 3 ，并且 从图像中计算出粒径。 如果颗粒相对于相机60的焦平面60a（参考符号A 1 ，A 3 ）偏离到前侧或后侧，则将该颗粒拍摄 大于实际状态，因此计算出的粒径变大。 在本发明中，确定每个颗粒的z方向的位置，计算偏差量Δz 1，Δz<3>，从而校正粒径。 因此，可以仅通过具有一个照相机的简单结构来确定每个颗粒在z方向上的位置，并且可以精确地测量粒度。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2012255679A

公开(公告)日：2012-12-27

申请号：JP2011127980

申请日：2011-06-08

申请人： Kowa Co ,  興和株式会社

发明人： HIRONO TAIRYO ,  ASANO TAKAHARU

IPC分类号： G01N33/579 ,  G01N21/47 ,  G01N33/53 ,  G01N33/543

摘要： PROBLEM TO BE SOLVED: To improve the accuracy of a measurement by performing the measurement without incurring coagulation or gelatinization which is not derived from a bio-based physiological substance, caused by stirring a mixture solution of an AL reagent and a sample when measuring the concentration of the physiological substance in the sample according to a light scattering method (including an AL bound beads method).SOLUTION: An AL reagent and a sample containing a bio-based physiological substance are mixed, light is made incident to a mixture solution and on the basis of the intensity of scattered light or transmitted light, the coagulation or gelatinization of protein caused by a reaction of the AL and the bio-based physiological substance in the mixture solution is detected. In place of stirring a mixture solution 7, an incident position of incident light is moved using a galvano mirror device 5 or the like.

摘要翻译： 要解决的问题：为了通过进行测量而提高测量的精度，而不会引起由生物基生理物质衍生的凝结或糊化，通过搅拌AL试剂和样品的混合溶液而引起的 根据光散射法（包括AL结合珠法）测定样品中生理物质的浓度。 解决方案：将AL试剂和含有生物基生理物质的样品混合，将光照入混合溶液，基于散射光或透射光的强度，引起蛋白质的凝结或凝胶化 通过AL和生物基生物物质在混合溶液中的反应被检测。 代替搅拌混合溶液7，使用电流镜装置5等移动入射光的入射位置。 版权所有（C）2013，JPO＆INPIT

公开(公告)号：JP2012215461A

公开(公告)日：2012-11-08

申请号：JP2011080714

申请日：2011-03-31

申请人： Kowa Co ,  興和株式会社

发明人： ASANO TAKAHARU ,  HIRONO TAIRYO ,  INADA KATSUYA

IPC分类号： G01N33/579 ,  G01N21/49 ,  G01N33/53 ,  G01N33/531 ,  G01N33/536

摘要： PROBLEM TO BE SOLVED: To improve measurement accuracy of measurement when measuring the concentration of a physiologically active substance derived from an organism in a sample by an agitation turbidimetric method, a light scattering method or an AL coupling bead method, by suppressing coagulation or gelatinization not derived from the physiologically active substance, which results in agitation of mixed liquid.SOLUTION: When mixing AL and a sample containing the physiologically active substance derived from an organism and detecting the coagulation of protein resulting in reaction of the AL and the physiologically active substance in the mixed liquid while agitating the mixed liquid, by adding a prescribed surfactant to the mixed liquid, the coagulation or gelatinization of the protein that does not result in the reaction of the AL and the physiologically active substance in the mixed liquid is suppressed.

摘要翻译： 要解决的问题：通过搅拌比浊法，光散射法或AL偶联珠法测定样品中来自生物的生理活性物质的浓度的测量精度，通过抑制凝血来提高测量精度 或不是衍生自生理活性物质的凝胶化，这导致混合液体的搅拌。 解决方案：当混合AL和含有衍生自生物体的生理活性物质的样品，并在搅拌混合液体的同时检测导致AL和生理活性物质在混合液体中的反应的蛋白质的凝结， 向混合液中配制表面活性剂，抑制不会导致AL与混合液中的生理活性物质的反应的蛋白质的凝结或凝胶化。 版权所有（C）2013，JPO＆INPIT