公开(公告)号：JPH06277040A

公开(公告)日：1994-10-04

申请号：JP10404391

申请日：1991-02-13

Applicant: NAT TAX ADMINISTRATION AGENCY ,  JOZO SHIGEN KENKYUSHO KK

Inventor： HARA MASAMICHI ,  TADENUMA MAKOTO ,  SATO SHUNICHI ,  IETOU HARUYUKI ,  SHIMOII HITOSHI ,  SUGIYAMA RYUICHI ,  TAMURA GAKUZO

IPC: C12N1/06 ,  C12N1/16 ,  C12N9/36 ,  C12R1/01

Abstract: PURPOSE:To selectively and efficiently lyse only the cell wall of a yeast without decomposing and denaturing its intracellular contents by allowing a microorganism having a yeast cell wall-lysing enzyme-producing ability or its cultured product to act on the yeast. CONSTITUTION:A microorganism having an ability of producing a yeast cell wall-lysing enzyme [e.g. a new microorganismic strain, YS-08 (FERM-P-11761)] and/or its culture product is allowed to act on a yeast, especially a basidiomycete such as a red yeast and/or a deuteromycete yeast. The yeast cell wall-lysing enzyme is preferably produced by inoculating the microorganism into a medium and subsequently culturing the microorganism at 25-35 deg.C at a pH of 6-7.

公开(公告)号：JPH02227192A

公开(公告)日：1990-09-10

申请号：JP32343788

申请日：1988-12-23

Applicant: NAT TAX ADMINISTRATION AGENCY ,  T D II KK

Inventor： YOSHIZAWA KIYOSHI ,  TADENUMA MAKOTO ,  SATO SHUNICHI ,  IETOU HARUYUKI ,  SHIMOII HITOSHI ,  SUZUKI OSAMU

IPC: C12N1/16 ,  C02F3/34 ,  C12R1/645

Abstract: PURPOSE:To save and conserve underground resources or water resources while reducing the use amount of cooling water and conserving energy by using yeast belonging to the genus Kluyveromyces and having heat resistance, flocculative properties and waste water treatment capacity. CONSTITUTION:Waste water of every kind, highly nutritive waste water, a diluted liquid thereof or a pretreated liquid thereof subjected to filtration, centrifugal separation or chemical treatment is inoculated with bacteria belonging to the genus Kluyveromyces to treat waste water. By treating even highly nutritive waste water difficult to treat heretofore with heat-resistant bacteria belonging to the genus Kluyveromyces, said waste water can be treated industrially in large quantities for the first time. By the use of said yeast, operation at high temp. wherein the liquid temp. of a yeast tank is as high as about 45 deg.C becomes possible for the first time and, as a result, the use amount of cooling water is reduced and the temp. of cooling water may be roughly controlled and the sharp conservation and saving of the energy of other cooling means can be achieved.

公开(公告)号：JPS63254978A

公开(公告)日：1988-10-21

申请号：JP8843887

申请日：1987-04-10

Applicant: NAT TAX ADMINISTRATION AGENCY ,  NAKAMURAYA CO LTD

Inventor： UEKI TOSHIHIRO ,  SAITO KAZUO ,  SHIMOII HITOSHI ,  SATO SHUNICHI ,  YAMAMOTO NAMI ,  TADENUMA MAKOTO ,  NISHIURA TAKATERU ,  YOSHINO HIROYUKI

IPC: A21D6/00 ,  A21D8/04 ,  C12N1/16 ,  C12N1/18 ,  C12R1/865

Abstract: PURPOSE:To obtain a freezing-resistant yeast having fermentation ability and flavor from a lysis-resistant strain, by repeating operations of lyzing baker's yeast with an yeast lytic enzyme, cultivating the residual yeast cells and then lyzing the yeast cells. CONSTITUTION:An adequate amount of baker's yeast is suspended in an yeast lytic enzyme solution containing preferably potassium chloride, sorbitol, etc., and lyzed for several hr. The residual microbial cells are then washed with sterile water. The live microbial cells are subsequently inoculated into a nutrient culture medium and cultivated to collect the microbial cells, which are washed. The residual yeast cells are then subjected to operation of similar lysis and cultivation several times to afford a lysis-resistant strain. A freezing-resistant yeast is obtained from the resultant lysis-resistant strain.

公开(公告)号：JPS63156597A

公开(公告)日：1988-06-29

申请号：JP30135086

申请日：1986-12-19

Applicant: NAT TAX ADMINISTRATION AGENCY

Inventor： SATO SHUNICHI ,  SHIMOII HITOSHI ,  SAITO KAZUO ,  TADENUMA MAKOTO ,  YOSHIZAWA KIYOSHI ,  OTANI MASAHIKO ,  NOSHIRO KIKUO

IPC: C02F3/34

Abstract: PURPOSE:To make efficient waste water treatment, by subjecting non- aggregating yeast for waste water treatment to a mutagenesis treatment then culturing the yeast, separating and growing the concd. aggregating variant and treating the waste water with the aggregating variant. CONSTITUTION:The non-aggregating yeast for waste water treatment (e.g., Hansenula Anolama J45-0) is subjected to the mutagenesis treatment such as UV irradiation and is then cultured. The fungus bodies settled quickly by gravity settlement or the fungus bodies sticking to the wall of a test tube are inoculated to a fresh medium and are cultured. The concd. aggregating variant is separated and grown by repeating the above-mentioned culture operation and the waste water is treated by such aggregating variant. As a result, the high yeast density is maintained and the org. materials in the waste water are efficiently decomposed; in addition, the nitrogen and phosphorus in the waste water are removed as well. Furthermore, this aggregating variant has the nature to stick well to carriers such as active carbon, polystyrene beads and disks and, therefore, the efficient waste water treatment is permitted by sticking the aggregating variant to the carrier.

公开(公告)号：JPS61153197A

公开(公告)日：1986-07-11

申请号：JP27273884

申请日：1984-12-26

Applicant: NAT TAX ADMINISTRATION AGENCY

Inventor： SAITO KAZUO ,  HASUO TETSUO ,  SATO SHUNICHI ,  YAMAMOTO NAMI ,  MAETANI TATSUO ,  TADENUMA MAKOTO ,  YOSHIZAWA KIYOSHI

IPC: C02F3/34 ,  C12N1/16 ,  C12R1/78

Abstract: PURPOSE:To remove org. materials contained in waste water by treating the waste water from a food industry, etc., with cohesive yeast. CONSTITUTION:Waste water from a food industry, etc., is treated with cohesive yeast Hansenula-Anomala J224 to remove org. materials in the waste water. In the waste water treatment by the cohesive yeast, the pH of the waste water is regulated in the wide range of 3-8, or preferably regulated to

公开(公告)号：JPH04158784A

公开(公告)日：1992-06-01

申请号：JP28448090

申请日：1990-10-24

Applicant: NAT TAX ADMINISTRATION AGENCY ,  JIYOUZOU SHIGEN KENKIYUUSHIYO

Inventor： HARA MASAMICHI ,  TADENUMA MAKOTO ,  SATO SHUNICHI ,  IETOU HARUYUKI ,  SHIMOII HITOSHI ,  SUGIYAMA RYUICHI ,  TAMURA GAKUZO

IPC: C12N9/24 ,  C12N1/16 ,  C12N15/04 ,  C12N15/10 ,  C12R1/01 ,  C12R1/645

Abstract: PURPOSE:To make it possible to selectively lyse only cell wall and lyse the title cell wall in such a way as to be useful for genetic engineering by acting a microorganism belonging to the genus Oerskovia and having yeast cell wall lysing enzyme producing ability or cultured mixture thereof on a yeast. CONSTITUTION:A yeast cell wall is lysed by acting a microorganism (e.g. FERM P-11737) belonging to the genus Oerskovia and having yeast cell wall lysing enzyme producing ability and/or cultured mixture thereof on a yeast. Furthermore, the microorganism has properties forming no Gram-positive bacillus and spore, having aerobic property and minute flagellum and having mobility and exhibiting no acid-fastness and culture thereof is preferably carried out at 25-35 deg.C and pH5-8 for 15-150 day when the microorganism is cultured.

公开(公告)号：JPH01224012A

公开(公告)日：1989-09-07

申请号：JP4585488

申请日：1988-03-01

Applicant: NAT TAX ADMINISTRATION AGENCY ,  TOHO DEV ENG

Inventor： YOSHIZAWA KIYOSHI ,  TADENUMA MAKOTO ,  SATO SHUNICHI ,  IEFUJI HARUYUKI ,  SHIMOII HITOSHI ,  SUZUKI OSAMU ,  HAMAZAKI KATSUJI ,  NITTA YOSHINOBU

IPC: B01D21/01 ,  C02F1/52 ,  C02F3/34

Abstract: PURPOSE:To enhance a solid-liq. separation effect on the waste water having a high content of org. matter with a simple method by treating the waste water, etc., from a food production process with flocculating yeast and a flocculant. CONSTITUTION:Waste water from a food production process is treated with flocculating yeast, and then added with a flocculant. Alternatively, the yeast and flocculant can be simultaneously added. Hansenula-anomala (The Microbiological Research Institute's report No.7671) can be exemplified as the yeast. The waste water treatment by the yeast is preferably carried out at pH3-8 and 10-36 deg.C. A cationic high molecular flocculant, an anionic high molecular flocculant, etc., are appropriately used as the flocculant, and the amt. of the flocculant to be added is appropriately controlled to 0.02-8% based on the solid matter.

公开(公告)号：JPH01191684A

公开(公告)日：1989-08-01

申请号：JP1470588

申请日：1988-01-27

Applicant: NAT TAX ADMINISTRATION AGENCY

Inventor： SHIMOII HITOSHI ,  KONDO HIROMOTO ,  IEFUJI HARUYUKI ,  SATO SHUNICHI ,  TADENUMA MAKOTO ,  HAYASHI MAKOTO ,  HARA MASAMICHI ,  YOSHIZAWA KIYOSHI

IPC: C12N9/80 ,  C12R1/38

Abstract: PURPOSE:To obtain the present urease capable of completely decomposing urea which causes the deterioration of liquors or unrefined liquors, by culturing a specific microbial strain capable of producing urease and separating the produced urease. CONSTITUTION:The objective urease can be produced by culturing a urease- producing microbial strain belonging to genus Pseudomonas and having optimum reaction pH in acidic region (e.g., FERM P-9793) and collecting the urease produced in the microbial cell and/or in the culture liquid. The culture is preferably carried out in a medium having a pH of 5.5-7.8 at about 30 deg.C for 5-72hr.

公开(公告)号：JPS63240775A

公开(公告)日：1988-10-06

申请号：JP18556587

申请日：1987-07-27

Applicant: NAT TAX ADMINISTRATION AGENCY ,  HOKKAIDO SUGAR CO

Inventor： SAITO KAZUO ,  SHIMOII HITOSHI ,  SATO SHUNICHI ,  TADENUMA MAKOTO ,  YOSHIZAWA KIYOSHI ,  MORIYA KAZUHITO ,  IZUMI CHIKASHI

IPC: C12P7/06 ,  C12N1/16 ,  C12R1/865

Abstract: NEW MATERIAL:Saccharomyces cerevisiae H-1 strain (FERM-P No.8885). USE:Useful for alcohol fermentation of beet molasses, having killer and aggregation properties, excellent continuous availability, reproducibility, etc., of fermenting power and capable of fermentation in charging in a high concentration when used for continuous method for utilization. PREPARATION:Saccharomyces cerevisiae 909-1 strain is initially sporulated and the formed spores are exposed and dispersed by a cell wall lytic enzyme. Colonies are then grown in a complete culture medium and then screened to separate haploids. Saccharomyces cerevisiae 180 strain is subsequently sporulated and the respective ascosporal quartets are joined to the above-mentioned haploids to cultivate the afore-mentioned zygotes.

公开(公告)号：JPH04365467A

公开(公告)日：1992-12-17

申请号：JP26307690

申请日：1990-10-02

Applicant: NAT TAX ADMINISTRATION AGENCY

Inventor： TADENUMA MAKOTO ,  KITAMURA HIDEFUMI ,  SATO SHUNICHI ,  IEFUJI HARUYUKI ,  SHIMOII HITOSHI

IPC: A23L35/00 ,  C12G3/02 ,  C12N1/16 ,  C12R1/865

Abstract: PURPOSE:To obtain a matured refined rice wine lees without change of unfavorable coloration, etc., due to maturation by autodigesting in a short time a specified temperature sensitive yeast obtained by mutagenesis treatment of the yeast in a refined rice wine lees. CONSTITUTION:A yeast, e.g. Saccharomyces cerevisiae J504 is initially subjected to mutagenesis treatment to obtain a temperature sensitive yeast normally growing within an allowable temperature range (10-30 deg.C) and performing alcohol fermentation while dying out and autodigesting in an unallowable temperature range (35-40 deg.C). The above-mentioned temperature sensitive yeast is then autodigested in a refined rice wine lees at a temperature within the unallowable temperature range to mature the refined rice wine lees in a short time. As the above-mentioned temperautre sensitive yeast, e.g. K-ts 8 strain (Bikokenstipulation No.11736) derived from Saccharomyces cerevisiae J504 is exemplified.