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1.发明专利
公开(公告)号：JPH06113821A
公开(公告)日：1994-04-26
申请号：JP29093592
申请日：1992-10-06
Applicant: NAT TAX ADMINISTRATION AGENCY , JOZO SHIGEN KENKYUSHO KK
Inventor： NISHITANI NAOMICHI , OBATA TAKAYUKI , IIMURA MINORU , YOSHIZAWA YUKIO , TAMURA GAKUZO
Abstract: PURPOSE:To obtain the subject yeast giving a liquor rich in fragrant component owing to the high productivity of the fragrant component independent of the kind of fatty acid in the raw material by separating a mutant strain which has become unable to utilize the fatty acids originated from the raw material by mutation. CONSTITUTION:The objective yeast is obtained by carrying out the mutation of a yeast and separating a mutant which has become unable to utilize the fatty acids originated from the raw material. Concretely, the KYOKAI No.7 sake yeast belonging to Saccharomyces cerevisiae is subjected to mutation treatment with ethylmethane sulfonate and cultured in a medium incorporated with cerulenin (an antibiotic substance specifically inhibiting the synthesis of fatty acid) and palmitic acid (a fatty acid) and the colonies unable to grow are separated to obtain the new mutant Saccharomyces cerevisiae K-7-CF4 (FERM P-13177).
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2.发明专利
公开(公告)号：JPH02117385A
公开(公告)日：1990-05-01
申请号：JP26809788
申请日：1988-10-26
Applicant: NAT TAX ADMINISTRATION AGENCY , JOZO SHIGEN KENKYUSHO KK
Inventor： TAKAHASHI KOJIRO , IIMURA MINORU , GOMI KATSUYA , HARA MASAMICHI , YOSHIZAWA KIYOSHI , MINAZU TETSUYOSHI , TAMURA GAKUZO
Abstract: PURPOSE:To eliminate production of urea in wines or alcohols by making a brewing using Saccharomyces cerevisiae transformed by a plasmid vector for alginase gene destruction of a plasmid vector for alginase gene replacement. CONSTITUTION:A plasmid vector for alginase gene destruction or a plasmid vector for alginase gene replacement is cut with a restriction enzyme into straight chain DNA. Using this DNA, Saccharomyces cerevisiae is transformed into a transformant causing no urea formation. Using this transformant, wine or an alcohol is produced. The above transformant, due to deletion of alginase, will inhibit urea formation, thus eliminating the formation of ethyl carbamate derived from urea, leading to production of wines, etc., free from ethyl carbamate (a carcinogen).
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公开(公告)号：JPH02268685A
公开(公告)日：1990-11-02
申请号：JP8678789
申请日：1989-04-07
Applicant: NAT TAX ADMINISTRATION AGENCY , JOZO SHIGEN KENKYUSHO KK
Inventor： TAKAHASHI KOJIRO , IIMURA MINORU , GOMI KATSUYA , HARA MASAMICHI , YOSHIZAWA KIYOSHI , TADA SETSUZO , TAMURA GAKUZO
Abstract: PURPOSE:To obtain DNA sequence containing a promoter, terminator and protein code region contained in amylase A gene isolated from Aspergillus oryzae and used for mass-producing Aspergillus oryzae by genetic engineering technique. CONSTITUTION:A DNA fragment coding takaamylase A is subjected to cloning from a cut fragment by restriction enzyme for genome DNA of Aspergillus oryzae and base sequence of the DNA fragment is determined. A transforming substance wherein takaamylase A activity is raised to six times can be obtained by transfecting a host vector system of Aspergillus oryzae, in which the DNA fragment is integrate, into Aspergillus oryzae.
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公开(公告)号：JPH02119779A
公开(公告)日：1990-05-07
申请号：JP27317288
申请日：1988-10-31
Applicant: NAT TAX ADMINISTRATION AGENCY , JOZO SHIGEN KENKYUSHO KK
Inventor： IIMURA MINORU , TAKAHASHI KOJIRO , GOMI KATSUYA , HARA MASAMICHI , YOSHIZAWA KIYOSHI , SHIBUYA ICHIRO , TAMURA GAKUZO
IPC: C12N9/34 , C12G3/02 , C12N1/15 , C12N1/19 , C12N15/09 , C12N15/56 , C12N15/80 , C12N15/81 , C12R1/66 , C12R1/69 , C12R1/865
Abstract: PURPOSE:To produce glucoamylase having ability separating a raw starch and capable of reacting in an acid area at high temperature by applying gene manipulation to a glucoamylase isolated from Aspergillus shirousamii. CONSTITUTION:DNA Fragment coding glucoamylase(GA) from genome DNA restriction enzyme fragment of Aspergillus shirousamii(AS) is cloned and integrated into a host vector system of Aspergillus oryzae to provide a transformant A capable of producing GA having 70 deg.C optimum reaction temperature and 3.2-7.5 reaction pH and reacting even at high temperature in an acidic area. Total mRNA of AS is further prepared and cDNA library is prepared based thereon and clone coding GA of AS is separated from within the library. The cDNA is coupled to an expression vector and then introduced into Saccharomyces cerevisiae to provide a transformant B. Both of the transformant A and B are used to provide the aimed GA.
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公开(公告)号：JPH0799979A
公开(公告)日：1995-04-18
申请号：JP26544793
申请日：1993-09-30
Applicant: NAT TAX ADMINISTRATION AGENCY , JOZO SHIGEN KENKYUSHO KK
Inventor： OBA TOSHITERU , SUDO SHIGETOSHI , KANEKO AKIHIRO , TAMURA GAKUZO
Abstract: PURPOSE:To obtain a new gene encoding alpha-amylase derived from Aspergillus kawachii, providing a large amount of an acid-resistant, thermostable and raw starch hydrolyzing alpha-amylase by gene manipulation, capable of directly producing an alcohol by transducing it into a yeast. CONSTITUTION:A conidium of Aspergillus kawachii IFO 4308 is inoculated into a medium and subjected to rotary shaking culture at 30 deg.C for 120 hours to give a mold. The prepared cell is collected by a filter, frozen with liquid nitrogen and ground and whole RNA is extracted from the ground cell by guanidine thiocyanate method and mRNA is isolated from the whole RNA by using an oligo alphaT column. Then the mRNA is used as a template to synthesize cDNA, a cDNA library is prepared by a conventional method and screened by using a DNA fragment containing part of a gene encoding an acid-resistant alpha-amylase as a probe to select a positive clone and to recover DNA, which is treated with a restriction enzyme to give the objective new gene encoding alpha-amylase having acid resistance, thermostability and raw starch hydrolyzing proprety.
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Patent Agency Ranking
公开(公告)号：JPH0662868A
公开(公告)日：1994-03-08
申请号：JP24011492
申请日：1992-08-18
Applicant: JOZO SHIGEN KENKYUSHO KK , NAT TAX ADMINISTRATION AGENCY
Inventor： MINETOKI TOSHIKI , TAMURA GAKUZO , GOMI KATSUYA , KITAMOTO KATSUHIKO , KUMAGAI CHIEKO
Abstract: PURPOSE:To produce an alpha-glucosidase in high efficiency by transforming Aspergillus oryzae with a vector of Aspergillus oryzae containing an alpha- glucosidase gene and culturing the transformed Aspergillus oryzae. CONSTITUTION:DNA fragment coding an alpha-glucosidase gene specified by a restriction map is cloned from a chromosome DNA of Aspergillus oryzae. Aspergillus oryzae is transformed by using the DNA fragment as a vector and the transformed Aspergillus oryzae is cultured in a medium containing maltose or starch as exclusive carbon source. The alpha-glucosidase secreted in the culture liquid is separated from the liquid. The obtained alpha-glucosidase is useful for the efficient production of e.g. isomaltooligosaccharide and utilizable as a delicious taste component of Japanese wine, fermented bean paste, soy sauce, etc., and for the proliferation of bifidus bacteria.
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公开(公告)号：JPH0595787A
公开(公告)日：1993-04-20
申请号：JP28411791
申请日：1991-10-04
Applicant: JOZO SHIGEN KENKYUSHO KK , NAT TAX ADMINISTRATION AGENCY
Inventor： OGAWA MASAHIRO , TAMURA GAKUZO , GOMI KATSUYA , KITAMOTO KATSUHIKO , KUMAGAI CHIEKO
Abstract: PURPOSE:To provide the subject promoter derived from genome gene of phosphoglycerate kinase of Aspergillus oryze, capable of mass-producing phosphoglycerate kinase and capable of efficiently producing various kinds of useful substances. CONSTITUTION:Aspergillus oryze R11340 strain is seeded in a culture medium, cultured at 30 deg.C for 3 day, subsequently collected, ground, suspended in a buffer solution, treated with phenol-chloroform and subjected to ethanol precipitation to isolate chromosome DNA. The isolated chromosome DNA is treated with a restriction enzyme and the resultant respective fragments are bonded to a vector to form a gene library of Aspergillus oryze chromosome DNA. The obtained gene library is subsequently screened by using a partial base sequence of phosphoglycerate kinase gene as a probe to select positive clones. From the selected positive clones, the plasmid is recovered and treated with a restriction enzyme, thus obtaining the objective phosphoglycerate kinase gene promoter.
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公开(公告)号：JPH06277040A
公开(公告)日：1994-10-04
申请号：JP10404391
申请日：1991-02-13
Applicant: NAT TAX ADMINISTRATION AGENCY , JOZO SHIGEN KENKYUSHO KK
Inventor： HARA MASAMICHI , TADENUMA MAKOTO , SATO SHUNICHI , IETOU HARUYUKI , SHIMOII HITOSHI , SUGIYAMA RYUICHI , TAMURA GAKUZO
Abstract: PURPOSE:To selectively and efficiently lyse only the cell wall of a yeast without decomposing and denaturing its intracellular contents by allowing a microorganism having a yeast cell wall-lysing enzyme-producing ability or its cultured product to act on the yeast. CONSTITUTION:A microorganism having an ability of producing a yeast cell wall-lysing enzyme [e.g. a new microorganismic strain, YS-08 (FERM-P-11761)] and/or its culture product is allowed to act on a yeast, especially a basidiomycete such as a red yeast and/or a deuteromycete yeast. The yeast cell wall-lysing enzyme is preferably produced by inoculating the microorganism into a medium and subsequently culturing the microorganism at 25-35 deg.C at a pH of 6-7.
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公开(公告)号：JPH02119783A
公开(公告)日：1990-05-07
申请号：JP27081988
申请日：1988-10-28
Applicant: NAT TAX ADMINISTRATION AGENCY , JOZO SHIGEN KENKYUSHO KK
Inventor： GOTO KUNIYASU , HARA MASAMICHI , YOSHIZAWA KIYOSHI , MOTOYOSHI TORU , TAMURA GAKUZO
Abstract: PURPOSE:To enable introduction of a giant DNA into a host yeast by using the protoplast fusion-accompanying transformation method. CONSTITUTION:Chromosomes are separated from a yeast and DNA derived from the separated chromosomes is recovered in a state of a giant DNA. The resultant DNA is directly introduced into a yeast using the pulse field gel electrophoresis method. The giant DNA is preferably obtained by separating the yeast chromosome DNA by molecular size using the pulse field gel electrophoresis method and recovering the separated chromosome DNA using an electrophoresis- like method. As the giant DNA for introduction, one obtained by mildly treating a giant DNA molecule with a restriction enzyme and then integrating a foreign gene thereinto can be used.
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公开(公告)号：JPH08275780A
公开(公告)日：1996-10-22
申请号：JP10703895
申请日：1995-04-07
Applicant: MEIJI MILK PROD CO LTD , NAT TAX ADMINISTRATION AGENCY
Inventor： ITO YOSHIYUKI , SASAKI TAKASHI , GOMI KATSUYA , KITAMOTO KATSUHIKO , TAKAHASHI KOJIRO , KUMAGAI CHIEKO , TAMURA GAKUZO
Abstract: PURPOSE: To obtain a new gene coding a β-galactosidase having a specific amino acid sequence and originated from Aspergillus oryzae, and giving a transformant highly expressing the above-mentioned enzyme useful for converting lactose into useful saccharides outside the cell. CONSTITUTION: This new gene codes a β-galactosidase having an amino acid sequence of the formula and originated from Aspergillus oryzae. The gene is useful for massively producing a transformant which highly expresses outside the 11 the K&B-galactosidase capable of converting lactose low in the utilization value into glucose and galactose which are high in the utilization values. The lactose is a main component in a whey by-produced in a process for producing cheese and casein. The gene is obtained by separating a chromosomal DNA from Aspergillus oryzae RIB 40 strain by a conventional method, making a chromosomal DNA library with the separated chromosomal DNA, screening the library with a product multiplied by a PCR method as a probe, selecting a positive clone and recovering the gene.
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