公开(公告)号：JP2007202569A

公开(公告)日：2007-08-16

申请号：JP2007127255

申请日：2007-05-11

Applicant: Roche Diagnostics Gmbh ,  ロシュ ダイアグノスティックス ゲーエムベーハー

Inventor： HEIDRICH BJOERN ,  ROBINSON PETER-NICHOLAS ,  TIECKE FRANK ,  ROLFS ARNDT

IPC: C12N15/09 ,  C12Q1/04 ,  C12Q1/68

CPC classification number: C12Q1/689 ,  Y02A50/451

Abstract: PROBLEM TO BE SOLVED: To provide a reagent comprising a fewer components and facilitating to detect Legionella, and a method for more specifically and more variously detecting Legionella. SOLUTION: Disclosed is a method for genus-specifically amplifying a nucleic acid of the genus Legionella, wherein a method featured by that all species having possibility of belonging to the genus Legionella are amplified by using primers less than 7; a method for detecting one or more species of the genus Legionella based on the amplified nucleic acid; and a reagent kit for genus-specific amplification and species-specific detection of Legionella species comprising one set of primers less than 7 and at least one Legionella species-specific probe are provided. COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 要解决的问题：提供一种试剂，其包含较少的组分并且便于检测军团菌，以及一种用于更具体地和更多地检测军团菌的方法。 解决方案：公开了一种专门扩增军团菌核酸的方法，其特征在于通过使用小于7的引物扩增具有属于军团菌属的可能性的所有物种的方法; 基于扩增的核酸检测军团菌属的一种或多种的方法; 以及用于属特异性扩增和物种特异性检测军团菌物种的试剂盒，其包含一组小于7的引物和至少一种军团菌物种特异性探针。 版权所有（C）2007，JPO＆INPIT