-
公开(公告)号：JP5896960B2
公开(公告)日：2016-03-30
申请号：JP2013140485
申请日：2013-07-04
发明人： ブラント ミハエル , フランツェ レインハルド , ペサラ ウルリッチ
IPC分类号： C07K14/505 , C12N5/10 , C12N15/09 , C12P21/02 , C12Q1/02
CPC分类号： C07K14/505 , C12N15/10 , C12N15/625 , A61K38/00 , C07K2319/02 , C07K2319/036 , C07K2319/75
-
2.发明专利Identification of human cell line for production of human protein by endogenous gene activation 有权通过内源性基因激活鉴定人类细胞系生产人蛋白质
公开(公告)号：JP2014193187A
公开(公告)日：2014-10-09
申请号：JP2014136448
申请日：2014-07-02
发明人： BRANDT MICHAEL , FRANZE REINHARD , PESSARA ULRICH
IPC分类号： C12Q1/02 , A61K38/00 , C07K14/505 , C12N5/08 , C12N15/09 , C12N15/10 , C12N15/62 , C12P21/02
CPC分类号： C07K14/505 , A61K38/00 , C07K2319/02 , C07K2319/036 , C07K2319/75 , C12N15/10 , C12N15/625
摘要： PROBLEM TO BE SOLVED: To provide a method for the selection of human cells for the production of human proteins by endogenous gene activation in order to produce human proteins in economical yields and in a form which is suitable for the production of a pharmaceutical preparation.SOLUTION: A method for the selection of human cell lines for the preparation of human proteins by the activation of a target gene endogenously present in a cell is characterized in that (a) a human cell line is tested for the presence of the following features: (i) the target gene with a desired nucleic acid sequence; (ii) at least 5 population doublings of the cell number within 14 days in a suspension culture; and (iii) at least 5 population doublings of the cell number within 14 days in a serum-free culture medium, and (b) using a cell line having features (i), (ii), and (iii) as starting cell line for the endogenous activation of the target gene.
摘要翻译： 要解决的问题：提供一种通过内源基因激活选择人细胞用于生产人类蛋白质的方法，以便以经济的产率和以适于制备药物制剂的形式产生人蛋白质。 ：通过活化内源存在于细胞中的靶基因来选择用于制备人蛋白质的人细胞系的方法的特征在于（a）测试人细胞系存在以下特征：（ i）具有所需核酸序列的靶基因; （ii）在悬浮培养中14天内至少5次细胞数量的倍增; 和（iii）在无血清培养基中在14天内至少5次细胞数量的倍增，和（b）使用具有特征（i），（ii）和（iii）的细胞系作为起始细胞系 用于靶基因的内源性激活。
-
公开(公告)号：JP5537894B2
公开(公告)日：2014-07-02
申请号：JP2009238436
申请日：2009-10-15
发明人： クライベル，イェルク , ヴァルテル，トーマス , ハルトティグ，ヘルベルト , レスニアク，クリストフ , メニク，マルティーン , リートリンク，ミヒャエル , シュミット，ヘルムート
CPC分类号： C23C30/00 , C12Q1/6806 , Y10S428/90 , Y10S435/814 , Y10T428/12042 , Y10T428/2996 , C12Q2563/149 , C12Q2563/143
摘要： Magnetic particles are prepared containing a magnetic core coated with a glass layer having a substantially pore-free glass surface or having pores with a diameter of less than 10 nm. The particles are used for separating biological material such as nucleic acids. A preferred process of preparing the particles is by forming a mixture of magnetic cores with a sol formed from an alcohol and a metal alkoxide, spray-drying the mixture to coat the cores with a layer of gelled sol, and heating the coated cores to obtain the magnetic glass particles. Preferably, the particles have an average particle size of less than 100 mum. The magnetic core may be a composite material containing a mica core and magnetite particles immobilized on the mica core, and the glass layer may contain boron oxide. Magnetic core materials include magnetite (Fe 3 O 4 ) and Fe 2 O 3 .
-
公开(公告)号：JP4756009B2
公开(公告)日：2011-08-24
申请号：JP2007157849
申请日：2007-06-14
发明人： ハルトティグ，ヘルベルト
CPC分类号： B03C1/01 , C08J3/124 , G01N33/5434 , G01N2446/80
-
公开(公告)号：JP4629813B2
公开(公告)日：2011-02-09
申请号：JP34223498
申请日：1998-12-01
发明人： スターン アン , ホノルド コンラッド , ホルトシェク トーマス
IPC分类号： C12N15/09 , C07K20060101 , C07K14/505 , C12N20060101 , C12N5/00 , C12N5/10 , C12N5/22 , C12N15/12 , C12N15/63 , C12N15/65 , C12N15/67 , C12N15/79 , C12N15/85 , C12N15/90 , C12Q20060101 , C12Q1/68 , C12R1/91
CPC分类号： C07K14/505 , C12N15/63 , C12N15/65 , C12N15/85 , C12N15/90 , C12N15/907 , C12N2800/30 , C12N2830/002 , C12Q1/6897
摘要： Preparing dihydrofolate reductase (DHFR)-negative eukaryotic cells (A), is new. Preparing dihydrofolate reductase (DHFR)-negative eukaryotic cells (A) comprises: (1) transfecting the cells with a vector that comprises at least one target sequence (TS) for a site-specific recombinase; sequences that flank TS and are homologous to a DHFR sequence endogenously present in the cell, to permit homologous recombination (HR); and optionally positive and/or negative selection marker genes (MG); (2) growing the transfected cells under conditions suitable for HR; and (3) recovering the resulting cells. Independent claims are also included for: (1) introducing a heterologous DHFR gene into a eukaryotic cell; (2) vector (V1) containing a sequence (X) that consists of an optional positive selection MG, sequence encoding DHFR and sequence encoding a desired protein, in expresssible form, where (X) is flanked by at least one TS; (3) vector (V2) for HR containing a sequence (Y) that consists of an optional positive selection MG, flanked by at least one TS, also sequences that flank (Y) and are homologous with a DHFR sequence endogenously present in the cell, to permit HR, optionally also a negative selection MG, outside the flanking sequences; (4) eukaryotic, preferably human, cells in which at least one endogenous DHFR-encoding sequence has been inactivated and, in this region, at least one TS has been integrated into the genome; and (5) eukaryotic, preferably human, cells that have a heterologous nucleic acid sequence (Z) in the region of an endogenous DHFR locus, where (Z) contains sequences encoding DHFR and a desired protein, also at least one TS.
-
公开(公告)号：JP4384400B2
公开(公告)日：2009-12-16
申请号：JP2002321415
申请日：2002-11-05
发明人： クラウス ウォーレリウス , ホルスト エベルハルツ , レインハード フランゼ
IPC分类号： C07K14/505 , C12P21/02 , A61K38/00 , C12N5/00 , C12N5/06 , C12N5/10 , C12N15/09 , C12N15/10 , C12N15/12 , C12N15/62 , C12P21/00
CPC分类号： C12N15/625 , A61K38/00 , C07K14/505 , C07K2319/02 , C07K2319/036 , C07K2319/75 , C12N15/10 , C12P21/005
摘要： The invention concerns a process for the production of a polypeptide with suitable glycosylation by culturing eukaryotic cells and isolating the polypeptide from the culture medium or/and the cells. In this process the desired glycosylated polypeptide can be produced recombinantly with the aid of endogenous gene activation or be produced naturally by the cells.
-
公开(公告)号：JP4373506B2
公开(公告)日：2009-11-25
申请号：JP20621798
申请日：1998-07-22
发明人： ニヒトル アルフォンス
IPC分类号： G01N33/531 , G01N33/553 , A61K47/30 , B01J13/00 , C07K14/00 , C07K17/14 , G01N33/543 , G01N33/58
CPC分类号： G01N33/585 , G01N33/553
-
公开(公告)号：JP4350235B2
公开(公告)日：2009-10-21
申请号：JP29028599
申请日：1999-10-12
发明人： ピスクル ジュル , イーレンフェルト ハンス−ジョルク , ミュンヒ−ピーターセン ブリジット , ソンダーガート ライフ
CPC分类号： C12N9/1205
-
9.发明专利
公开(公告)号：JP2009075125A
公开(公告)日：2009-04-09
申请号：JP2008295166
申请日：2008-11-19
发明人： KARL JOHANN , LENZ HELMUT , KRAUSE FRIEDEMANN , FINCKH PETER , HORNAUER HANS , BERGER JOHANN
IPC分类号： G01N33/543 , C12Q1/68 , G01N33/566
CPC分类号： G01N33/54366 , C12Q1/6834 , Y10S435/805 , Y10S435/81 , Y10S435/962 , Y10S435/973 , Y10S435/974
摘要： PROBLEM TO BE SOLVED: To provide a device and a method for detecting an analyte, capable of displaying directly the presence of interference to take the interference into consideration, when evaluating a test result, and capable of displaying directly a type of the interference in some cases.
SOLUTION: A solid phase has at least one test area containing a reagent for detecting at least the one analyte in a sample, and further includes at least one control area for detecting an interference reaction due to nonspecific coupling of a nonanalyte interference component onto the test area. A solid phase receptor having an analyte specific coupling segment is applied using a filling solution, and the control area contains the reagent of the filling solution, but includes no analyte specific coupling segment.
COPYRIGHT: (C)2009,JPO&INPIT摘要翻译： 要解决的问题：为了提供一种用于检测分析物的装置和方法，其能够在评估测试结果时直接显示干扰的存在以考虑干扰，并且能够直接显示一种类型的 有些情况下有干扰。 解决方案：固相具有至少一个包含用于检测样品中至少一种分析物的试剂的测试区域，并且还包括至少一个控制区域，用于检测由非分析干扰组分的非特异性偶联引起的干扰反应 到测试区域。 使用填充溶液施加具有分析物特异性偶联区段的固相受体，并且控制区域含有填充溶液的试剂，但不包括分析物特异性偶联区段。 版权所有（C）2009，JPO＆INPIT
-
公开(公告)号：JP2007202569A
公开(公告)日：2007-08-16
申请号：JP2007127255
申请日：2007-05-11
CPC分类号： C12Q1/689 , Y02A50/451
摘要： PROBLEM TO BE SOLVED: To provide a reagent comprising a fewer components and facilitating to detect Legionella, and a method for more specifically and more variously detecting Legionella. SOLUTION: Disclosed is a method for genus-specifically amplifying a nucleic acid of the genus Legionella, wherein a method featured by that all species having possibility of belonging to the genus Legionella are amplified by using primers less than 7; a method for detecting one or more species of the genus Legionella based on the amplified nucleic acid; and a reagent kit for genus-specific amplification and species-specific detection of Legionella species comprising one set of primers less than 7 and at least one Legionella species-specific probe are provided. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译： 要解决的问题：提供一种试剂，其包含较少的组分并且便于检测军团菌，以及一种用于更具体地和更多地检测军团菌的方法。 解决方案：公开了一种专门扩增军团菌核酸的方法，其特征在于通过使用小于7的引物扩增具有属于军团菌属的可能性的所有物种的方法; 基于扩增的核酸检测军团菌属的一种或多种的方法; 以及用于属特异性扩增和物种特异性检测军团菌物种的试剂盒，其包含一组小于7的引物和至少一种军团菌物种特异性探针。 版权所有（C）2007，JPO＆INPIT
-
-
-
-
-
-
-
-
-
搜索结果
134 条记录 (耗时 0.032 秒)
