公开(公告)号：JP2006025651A

公开(公告)日：2006-02-02

申请号：JP2004206746

申请日：2004-07-14

Applicant: Sds Biotech Corp ,  株式会社エス・ディー・エス バイオテック

Inventor： TANABE HIROSHI ,  YAMANAKA SATOSHI ,  NAGASAKI KEIZO ,  TOMARU YUJI

IPC: C12N7/00 ,  A01N25/22 ,  A01N37/18 ,  A01N63/00 ,  A01N63/02

Abstract: PROBLEM TO BE SOLVED: To provide a method for improving storage stability of an algaecidal virus against red tide, in order to prevent reduction in algaecidal ability to algae to be infection object, since a large-sized DNA virus having algaecidal activity against red tide-causing algae is readily deactivated by light and a water temperature, although activity is kept to some extent by preservation under light shielding and low-temperature conditions and in order to further improve preservability, wherein antibiotics and glycerol as a protecting agent are added to the algaecidal virus against red tide.
SOLUTION: The method for improving storage stability of the virus comprises adding streptomycin sulfate (50-5,000 ppm) and chloramphenicol (5-500 ppm) and glycerol (1-20%) to a suspension of HaV (Heterosigma akashiwo virus) being an algaecidal virus against Heterosigma akashiwo of harmful red tide-causing alga and HcV (Heterocapsa circularisquama virus) being an algaecidal virus against Heterocapsa circularisquama.
COPYRIGHT: (C)2006,JPO&NCIPI

Abstract translation: 待解决的问题：提供一种提高灭藻病毒抗红潮的贮藏稳定性的方法，以防止将藻类杀藻能力降低为感染对象，因为具有杀藻活性的大尺寸DNA病毒 红潮引起的藻类在光和水温下容易失活，尽管活性通过在遮光和低温条件下保存而保持一定程度，并为了进一步改善保存性，其中加入抗生素和甘油作为保护剂 对抗潮湿的灭藻病毒。 解决方案：用于提高病毒储存稳定性的方法包括将硫酸链霉素（50-5,000ppm）和氯霉素（5-500ppm）和甘油（1-20％）加入到HaV（异基因二病毒）的悬浮液中， 是一种抗Heterosigma akashiwo的有害红潮引起的藻类和HcV（Heterocapsa circularisquama病毒）的灭藻病毒，是抗Heterocapsa circularisquama的灭藻病毒。 版权所有（C）2006，JPO＆NCIPI

公开(公告)号：JP2009195117A

公开(公告)日：2009-09-03

申请号：JP2008037662

申请日：2008-02-19

Applicant: Sds Biotech Corp ,  株式会社エス・ディー・エス バイオテック

Inventor： NAGASAKI KEIZO ,  TOMARU YUJI ,  NAGAI SATOSHI ,  TAKAO YOSHITAKE ,  TANABE HIROSHI

IPC: C12N7/00 ,  A01N63/00 ,  A01P7/04 ,  A01P13/00

Abstract: PROBLEM TO BE SOLVED: To provide a new virus which specifically infects algae in Cryptophyte, Teleaulax and proliferates.
SOLUTION: This virus having the following forms and biological properties infects algae in Cryptophyte, Teleaulax and proliferates to control or inhibit the proliferation of the algae. (Form) total length: 0.01 to 0.4 μm; shape of the virus: spherical; particle size: 130 to 260 nm; the structure of a tail portion, and the structure of an outer membrane are absent; shape of capsid: regular icosahedron; genome: double-strand DNA. (Biological properties) the virus does not exhibit infectivity or alga solubility to the algae of Bacillariophyceae, Eustigmatophyceae, Dinophyceae and Raphidophyceae classes.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供特异性感染Cryptophyte，Teleaulax中的藻类并增殖的新病毒。 解决方案：具有以下形式和生物学特性的病毒感染Cryptophyte，Teleaulax中的藻类并增殖以控制或抑制藻类的增殖。 （形式）总长度：0.01〜0.4μm; 形状的病毒：球形; 粒度：130〜260nm; 尾部的结构和外膜的结构不存在; 衣壳形状：常规二十面体; 基因组：双链DNA。 （生物学特性）病毒不表现出对芽孢杆菌藻，ust螨科，D螨科和Raphidophyceae类的藻类的感染性或藻类溶解度。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2006254904A

公开(公告)日：2006-09-28

申请号：JP2006037263

申请日：2006-02-14

Applicant: Fisheries Research Agency ,  Fukui Prefecture ,  Sds Biotech Corp ,  株式会社エス・ディー・エス バイオテック ,  独立行政法人水産総合研究センター ,  福井県

Inventor： NAGASAKI KEIZO ,  YOSHIDA TAKASHI ,  HIROISHI SHINGO ,  TAKASHIMA YUKARI ,  TOMARU YUJI ,  DOMEN YOKO ,  TANABE HIROSHI ,  YAMANAKA SATOSHI ,  SHISA TOMOKO ,  TAKAO YOSHITAKE

IPC: C12N7/00 ,  A01N63/00 ,  A01P13/00 ,  C12N7/02 ,  C12N7/08 ,  C12N15/09 ,  C12R1/94

Abstract: PROBLEM TO BE SOLVED: To provide cyanophage capable of specifically infecting the water bloom-causing blue-green algae of the genus Microcystis and proliferating, to provide a method for controlling water bloom with the cyanophage, to provide an agent for controlling water bloom, to provide a method for isolating the cyanophage, to provide a method for concentrating/purifying, to provide a subculture method, and to provide a preservation method.
SOLUTION: The cyanophage having a total length of 0.01 to 0.4 μm and capable of specifically infecting the algae of the genus Microcystis and proliferating. The method for isolating the cyanophage includes a process for filtering a liquid sample containing the cyanophage with a filter, inoculating the obtained filtrate into a solution for culturing the algae of the genus Microcystis, culturing the cyanophage, and then subjecting the alga dissolution-observed culture solution to a limiting dilution treatment to clone the cyanophage. The agent for controlling water bloom contains the cyanophage as an active ingredient. The method for controlling water bloom comprises spraying the cyanophage on a water area. The method for concentrating/purifying the cyanophage, the subculture method, and the preservation method.
COPYRIGHT: (C)2006,JPO&NCIPI

Abstract translation: 要解决的问题：为了提供能够特异性感染微囊藻类的引起水华的蓝绿色藻类并增殖的氰染色体，以提供一种用于控制水雾的方法，以提供用于控制水的药剂 提供分离蓝细菌的方法，提供浓缩/纯化方法，提供传代培养方法，并提供保存方法。 解决方案：全长为0.01〜0.4μm，能够特异性感染微囊藻类并增殖的藻类。 用于分离蓝藻的方法包括用过滤器过滤含有氰染色液体样品的方法，将获得的滤液接种到用于培养微囊藻属藻类的溶液中，培养蓝藻，然后对藻类溶解观察培养物 溶液进行有限稀释处理以克隆蓝藻。 用于控制水华的药剂含有蓝藻作为活性成分。 控制水华的方法包括在水域喷洒蓝藻。 浓缩/净化蓝藻的方法，传代培养方法和保存方法。 版权所有（C）2006，JPO＆NCIPI

公开(公告)号：JP2006025652A

公开(公告)日：2006-02-02

申请号：JP2004206763

申请日：2004-07-14

Applicant: Sds Biotech Corp ,  株式会社エス・ディー・エス バイオテック

Inventor： TANABE HIROSHI ,  YAMANAKA SATOSHI ,  NAGASAKI KEIZO ,  TOMARU YUJI

IPC: C12N7/00

Abstract: PROBLEM TO BE SOLVED: To provide a method for concentratedly obtaining high-density viruses in a short period of time in artificially mass producing useful algaecidal viruses against red tide-causing algae.
SOLUTION: The method for concentratedly obtaining high-density viruses comprises controlling HaV (Heterosigma akashiwo virus) being an algaecidal virus of Heterosigma akashiwo of harmful red tide-causing alga and HcV (Heterocapsa circularisquama virus) being an algaecidal virus of Heterocapsa circularisquama by the amounts and the species of viruses to be inoculated to each culture solution of host plankton.
COPYRIGHT: (C)2006,JPO&NCIPI

Abstract translation: 待解决的问题：提供一种在短时间内集中获得高密度病毒以人造大规模生产用于抗红潮引起的藻类的有效的杀藻病毒的方法。 解决方案：集中获得高密度病毒的方法包括控制作为有害的红潮引起的藻类的异igma病的灭藻病毒（Heterosigma akashiwo病毒）和作为Heterocapsa circularisquama的灭藻病毒的HcV（Heterocapsa circularisquama病毒）的HaV 通过接种到宿主浮游生物的每种培养液中的病毒的数量和种类。 版权所有（C）2006，JPO＆NCIPI

公开(公告)号：JP2004105095A

公开(公告)日：2004-04-08

申请号：JP2002272413

申请日：2002-09-19

Applicant: Fisheries Research Agency ,  Sds Biotech:Kk ,  株式会社エス・ディー・エス バイオテック ,  独立行政法人水産総合研究センター

Inventor： NAGASAKI KEIZO ,  YAMAGUCHI MINEO ,  TOMARU YUJI ,  KATANOSAKA NORIAKI ,  ITAKURA SHIGERU

IPC: A01G33/02 ,  A01H13/00 ,  A01M21/00 ,  C12N7/00 ,  C12N7/02

Abstract: PROBLEM TO BE SOLVED: To provide virus growable/algilytic by specifically infecting on red tide-derived diatom Rhizosolenia, to provide a method and an agent for controlling the red tide using the virus, and to provide respective methods for isolating, subculturing and storing the virus.
SOLUTION: The virus is smaller than 0.1μm in particle size. The method for isolating the virus comprises filtering a liquid sample containing the virus via a 0.1μm-perforation filter, inoculating the filtrate into a culture fluid of diatom Rhizosolenia to culture the diatom, and carrying out a limiting dilution of the resultant culture fluid where an algilysis of the diatom is observed to clone the virus. The red tide-controlling agent comprises the virus as the active ingredient. The method for controlling the red tide comprises spreading red tide waters with the virus. The respective methods for isolating, subculturing and storing the virus are also provided.
COPYRIGHT: (C)2004,JPO

Abstract translation: 待解决的问题：为了提供通过特异性感染来自红潮的硅藻Rhizosolenia的可生长/耐盐性的病毒，提供使用病毒控制红潮的方法和试剂，并提供各种分离，传代培养的方法 并存储病毒。

解决方案：病毒粒径小于0.1μm。 分离病毒的方法包括通过0.1μm穿孔过滤器过滤含有病毒的液体样品，将滤液接种到硅藻Rhizosolenia的培养液中以培养硅藻，并对所得培养液进行有限稀释，其中 观察到硅藻的裂解以克隆病毒。 红潮控制剂包括病毒作为活性成分。 控制红潮的方法包括用病毒传播红潮水。 还提供了分离，传代和存储病毒的各种方法。 版权所有（C）2004，JPO