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308 条记录 (耗时 0.019 秒)

    FREEZE-PRESERVING LIQUID FOR BACTERIA
    6.
    发明专利
    FREEZE-PRESERVING LIQUID FOR BACTERIA 失效

    公开(公告)号：JP2001327280A

    公开(公告)日：2001-11-27

    申请号：JP2000190271

    申请日：2000-05-23

    申请人： LION CORP

    发明人： KAWAGUCHI HIROMI ,  IZUMIKAWA YOSUKE ,  ITO SATOSHI ,  TANAKA KENSUKE

    IPC分类号： C12N1/04 ,  C12N1/20 ,  C12R1/385

    摘要： PROBLEM TO BE SOLVED: To provide a freeze-preserving liquid for bacteria, exhibiting a good bacterial viability and activity after their preservation and being provided for a preservation efficacy test, a bactericidal capacity test and an antibacterial test directly without performing a pre-culture. SOLUTION: This freeze-preserving liquid for bacteria is characterized by containing trehalose and/or a polyethylene glycol as active ingredients.

    METHOD FOR SCREENING INHIBITORY SUBSTANCE FOR PSEUDOMONAS AERUGINOSA IRON-CAPTURING SYSTEM
    7.
    发明专利
    METHOD FOR SCREENING INHIBITORY SUBSTANCE FOR PSEUDOMONAS AERUGINOSA IRON-CAPTURING SYSTEM 失效

    公开(公告)号：JP2001299380A

    公开(公告)日：2001-10-30

    申请号：JP2000118179

    申请日：2000-04-19

    申请人： DAIICHI SEIYAKU CO

    发明人： TAKASE HIROYUKI ,  NIUCHI HIRONOBU

    IPC分类号： G01N33/50 ,  A61K45/00 ,  A61P31/04 ,  C12Q1/04 ,  C12Q1/18 ,  C12R1/385 ,  G01N33/15

    摘要： PROBLEM TO BE SOLVED: To provide a method for capable of screening an inhibitory substance for pseudomonas aeruginosa iron-capturing system, useful for creating an anti- pseudomonas aeruginosa agent or anti-pseudomonas aeruginosa infection agent based on a new action mechanism. SOLUTION: This method for screening the inhibitory substance for pseudomonas aeruginosa iron-capturing system comprises the following processes (1) and (2): (1) a process of adding a substance to be tested into each of the systems of culturing pseudomonas aeruginosa by using (A) a medium in which a TonB-lacking strain of the pseudomonas aeruginosa can propagate and (B) a medium in which the TonB-lacking strain can not propagate; and (2) a process of selecting a substance which does not inhibit the propagation of the pseudomonas aeruginosa in the above medium (A), but inhibits the propagation of the pseudomonas aeruginosa in the above medium (B).

    DETECTION OF DNA HAVING MICROMUTATION
    8.
    发明专利
    DETECTION OF DNA HAVING MICROMUTATION 失效

    公开(公告)号：JP2000308489A

    公开(公告)日：2000-11-07

    申请号：JP12195799

    申请日：1999-04-28

    申请人： DAIICHI SEIYAKU CO

    发明人： YOKOTA HIROSHI ,  GOTO KOSHICHI

    IPC分类号： C12N15/09 ,  C12N15/00 ,  C12Q1/68 ,  C12R1/385

    摘要： PROBLEM TO BE SOLVED: To provide a method for detecting DNA having micromutation by combining a procedure of condensing a DNA part having structural characteristics or periphery thereof using a substance which specifically recognizes such a DNA, and a procedure of cloning by the subtraction method. SOLUTION: This is a method for cloning a DNA part having structural characteristics or periphery thereof containing such a DNA part by performing a method including, in combination, a process of condensing a DNA part having structural characteristics or periphery thereof containing the DNA part by utilizing a substance which specifically recognizes such a DNA, and a process of selectively cloning only a DNA part having structural characteristics or periphery thereof containing the DNA part by the subtraction treatment.

    LIPOPROTEIN I IN PSEUDOMONAS AERUGINOSA
    9.
    发明专利
    LIPOPROTEIN I IN PSEUDOMONAS AERUGINOSA 失效

    公开(公告)号：JP2000152794A

    公开(公告)日：2000-06-06

    申请号：JP20692199

    申请日：1999-07-21

    申请人： CHIRON BEHRING GMBH & CO

    发明人： DUCHENE MICHAEL ,  VON SPECHT ULRICH ,  DOMDEY HORST

    IPC分类号： C12N15/09 ,  A61K39/104 ,  A61K39/395 ,  A61K39/40 ,  A61P31/04 ,  C07H21/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/21 ,  C07K14/41 ,  C07K14/705 ,  C07K16/00 ,  C07K16/12 ,  C12N15/31 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/68 ,  C12Q1/689 ,  C12R1/19 ,  C12R1/385 ,  G01N33/569 ,  G01N33/577

    摘要： PROBLEM TO BE SOLVED: To obtain a new immunogenicity partial sequence substance of lipoprotein I in Pseudomonas aeruginosa which consists of an immunogenicity partial sequence substance of adventitial protein I in Pseudomonas aeruginosa containing a specific amino acid sequence and is used as an effective vaccine or the like in the protection or the like due to the active immunity against pyocyanic infections. SOLUTION: This immunogenicity partial sequence substance is a new immunogenicity partial sequence substance of adventitial protein (lipoprotein) I in Pseudomonas aeruginosa containing the amino acid sequence represented by the formula and is useful as an effective vaccine or the like in the protection or the like due to the active immunity against pyocyanic infections. This immunogenicity partial sequence substance is obtained by initially isolating and purifying a genome DNA by means of a conventional method from Pseudomonas aeruginosa, preparing a gene bank by using this genome DNA followed by plating this gene bank, transfering materials from the resultant plaque to a nitrocellulose membrane, detecting a plaque which expresses a small amount of lipoprotein I by reacting a monoclonal antibody peculiar to the lipoprotein I in Pseudomonas aeruginosa and finally recovering the gene from this positive plaque.

    10.
    发明专利
    失效

    公开(公告)号：JP3042015B2

    公开(公告)日：2000-05-15

    申请号：JP12472891

    申请日：1991-04-26

    IPC分类号： C12Q1/04 ,  C12R1/385 ,  C12R1/39