公开(公告)号：US20140106989A1

公开(公告)日：2014-04-17

申请号：US14056162

申请日：2013-10-17

Applicant: Bio-Rad Laboratories, Inc., LSG - LSD Division

Inventor： John Barich ,  Renee Lemaire-Adkins ,  David Neaz ,  Ariel Notcovich ,  Paul Patt ,  Ryan Short ,  Steven Swihart ,  Evan Thrush

IPC: G01N21/63

Abstract: Analyte arrays such as solutes in a slab-shaped gel following electrophoresis, and particularly arrays that are in excess of 3 cm square and up to 25 cm square and higher, are imaged at distances of 5 cm or less by either forming sub-images of the entire array and stitching together the sub-images by computer-based stitching technology, or by using an array of thin-film photoresponsive elements that is coextensive with the analyte array to form a single image of the array.

Abstract translation: 分析物阵列如电泳后的板状凝胶中的溶质，特别是超过3cm见方和高达25cm见方的高达25cm见方的阵列，通过以下方式成像5cm或更小的距离： 整个阵列并通过基于计算机的缝合技术将子图像拼接在一起，或者通过使用与分析物阵列共同延伸以形成阵列的单个图像的薄膜光响应元件阵列来拼接子图像。

公开(公告)号：US09606111B2

公开(公告)日：2017-03-28

申请号：US13870710

申请日：2013-04-25

Applicant: Bio-Rad Laboratories, Inc., LSG-LSD Division

Inventor： Steve Freeby ,  Ning Liu ,  Kevin McDonald ,  Aran Paulus ,  Anton Posch

IPC: G01N33/53 ,  G01N33/68

CPC classification number: G01N33/53 ,  G01N33/6827 ,  G06F19/10 ,  G06T7/0012 ,  G06T11/60 ,  G06T2207/30004

Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

公开(公告)号：US20150070483A1

公开(公告)日：2015-03-12

申请号：US14477253

申请日：2014-09-04

Applicant: Bio-Rad Laboratories, Inc., LSG-LSD Division

Inventor： Evan Thrush ,  Evelio Perez ,  Steve Swihart

IPC: G02B21/36 ,  G02B21/00 ,  G02B21/04

CPC classification number: G02B21/361 ,  G01N21/00 ,  G01N21/76 ,  G02B17/023 ,  G02B21/0008 ,  G03B15/00 ,  G03B17/17

Abstract: An imaging assembly for the viewing, imaging, and analysis of biological, chemical, and/or biochemical samples in gels or other substrates, in which an adjustable camera and lens module, a reflex mirror, and a focal plane mirror, are configured to bend or fold an optical path in order to image a target region, and where the optical path can be reflected along non-orthogonal angles. The imaging assembly is configured to reduce the overall size of the imaging apparatus due to the angles at which the mirrors and camera and lens assembly are positioned relative to each other, which allows for the imaging of relatively larger samples in the target region.

Abstract translation: 用于观察，成像和分析凝胶或其它基底中的生物，化学和/或生化样品的成像组件，其中可调节的相机和透镜模块，反射镜和焦平面镜配置成弯曲 或折叠光路以便对目标区域进行成像，并且其中可以沿非正交角度反射光路。 成像组件被配置为由于反射镜和相机和透镜组件相对于彼此定位的角度而减小成像装置的总体尺寸，这允许在目标区域中对相对较大的样本进行成像。

公开(公告)号：US20130288388A1

公开(公告)日：2013-10-31

申请号：US13870710

申请日：2013-04-25

Applicant: BIO-RAD LABORATORIES, INC., LSG - LSD DIVISION

Inventor： Steve Freeby ,  Ning Liu ,  Kevin McDonald ,  Aran Paulus ,  Anton Posch

IPC: G01N33/53

CPC classification number: G01N33/53 ,  G01N33/6827 ,  G06F19/10 ,  G06T7/0012 ,  G06T11/60 ,  G06T2207/30004

Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

Abstract translation: 本文公开了使用卤代烷基化色氨酸荧光进行蛋白质定量和归一化的方法。 使用与色氨酸残基反应以形成荧光产物的卤素取代的有机化合物（即卤代烷）处理复合蛋白质样品，即每种含有来自不同来源的不具有共同蛋白质谱的1,000个或更多个不同蛋白质的样品。 然后使用紫外光照射样品，并对每个样品中所有蛋白质产生的荧光发射进行检测和定量，以获得各种样品中总蛋白质含量的比较值。 这样获得的值被发现是比较总蛋白质含量的有效指示，尽管事实上，任何单个样品中的各种蛋白质之间的色氨酸水平变化很大，并且样品由于其起源的多样性而趋于彼此不同 在它们所含的蛋白质的身份和相对量中。 蛋白质样品也通过使用每个样品中的总蛋白的无染色检测或每个样品中的子样品检测来校准样品稀释度，样品加载和蛋白质转移不一致性的差异。