公开(公告)号：US08187837B2

公开(公告)日：2012-05-29

申请号：US12670734

申请日：2008-06-20

申请人： Ute Ehringer ,  Eva Kohlstrung

发明人： Ute Ehringer ,  Eva Kohlstrung

IPC分类号： C12P21/00 ,  C12N1/06 ,  C12N1/21 ,  C07K14/51

CPC分类号： C07K14/475

摘要： A process for the production of a purified recombinant GDF-5 related protein in prokaryotes comprises the steps of bacterial cell disruption and inclusion body solubilization to obtain a solubilized monomer of a GDF-5 related protein, said process characterized by a) disruption of bacterial cells with a high pressure homogenizer at a disruption pressure between 800 and 900 bar; and/or b) treatment of the recovered inclusion bodies with a denaturing solubilization buffer comprising L-arginine.

摘要翻译： 在原核生物中生产纯化的重组GDF-5相关蛋白的方法包括细菌细胞破坏和包涵体增溶步骤以获得GDF-5相关蛋白的溶解单体，所述方法的特征在于a）细菌细胞的破坏 在高压均化器处于800至900巴之间的破碎压力下; 和/或b）用包含L-精氨酸的变性增溶缓冲液处理回收的包涵体。

公开(公告)号：US20110160441A1

公开(公告)日：2011-06-30

申请号：US12670734

申请日：2008-06-20

申请人： Ute Ehringer ,  Eva Kohlstrung

发明人： Ute Ehringer ,  Eva Kohlstrung

IPC分类号： C07K14/475

CPC分类号： C07K14/475

摘要： A process for the production of a purified recombinant GDF-5 related protein in prokaryotes comprises the steps of bacterial cell disruption and inclusion body solubilization to obtain a solubilized monomer of a GDF-5 related protein, said process characterized by a) disruption of bacterial cells with a high pressure homogenizer at a disruption pressure between 800 and 900 bar; and/or b) treatment of the recovered inclusion bodies with a denaturing solubilization buffer comprising L-arginine.

摘要翻译： 在原核生物中生产纯化的重组GDF-5相关蛋白的方法包括细菌细胞破坏和包涵体增溶步骤以获得GDF-5相关蛋白的溶解单体，所述方法的特征在于a）细菌细胞的破坏 在高压均化器处于800至900巴之间的破碎压力下; 和/或b）用包含L-精氨酸的变性增溶缓冲液处理回收的包涵体。