公开(公告)号：US20060099611A1

公开(公告)日：2006-05-11

申请号：US11156014

申请日：2005-06-17

申请人： Robert Irving ,  Peter Hudson ,  Huseyin Mustafa ,  Kim Wark ,  Matias Abregu

发明人： Robert Irving ,  Peter Hudson ,  Huseyin Mustafa ,  Kim Wark ,  Matias Abregu

IPC分类号： C12Q1/68 ,  C12P21/06 ,  C12N1/21 ,  C12N1/18 ,  C12N5/06

CPC分类号： C40B40/02 ,  C12N15/102 ,  C12N15/1037

摘要： The present invention relates to the field of evolution of nucleic acids in vivo and provides methods and compositions for introducing diversity into gene products. The present invention allows generation of new sequences that have desirable properties by virtue of high frequency mutation events within a cell. The high frequency mutation of a polynucleotide sequence results in, the production of a large population of new sequence variants. Appropriate selection and/or screening permits identification and isolation of mutant forms of the polynucleotide sequence as well as products resulting from expression of the mutant sequences.

摘要翻译： 本发明涉及体内核酸进化领域，并提供了将多样性引入基因产物的方法和组合物。 本发明允许通过细胞内的高频突变事件产生具有期望特性的新序列。 多核苷酸序列的高频突变导致大量新序列变体的产生。 适当的选择和/或筛选允许鉴定和分离突变形式的多核苷酸序列以及由突变序列的表达产生的产物。