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公开(公告)号：US12091656B2
公开(公告)日：2024-09-17
申请号：US16472645
申请日：2017-12-27
发明人： Manuel Kaulich , Andreas Ernst , Martin Wegner , Valentina Diehl , Rahel De Bruyn , Svenja Wiechmann
CPC分类号： C12N15/102 , C07H21/02 , C07H21/04 , C12N15/1037 , C12N15/1072 , C40B20/04 , C40B30/04 , C40B40/02 , C40B40/06 , C12Y207/07007 , C12Y302/02027 , C12Y605/01001
摘要： The present invention pertains to a novel method for the generation of highly diverse RNA expressing vectors and vector libraries for use in targeted gene knock out, knock down and genome modification approaches. The invention pertains to a method for generating such higher order libraries without the need of classical cloning technologies. This is particularly useful for libraries based on large vectors wherein a sequence cannot be easily mutated with classical mutagenesis methods. The vectors and libraries generated according to the methods of the invention are in particular for RNA assisted silencing technologies such as RNA interference, and for targeted genome editing using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system or similar RNA/DNA-encoded gene perturbation systems which use small guide RNAs to target the CRISPR complex to a specific genomic sequence. The invention provides also kits comprising the materials for performing the methods of the invention.
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公开(公告)号：US12084651B2
公开(公告)日：2024-09-10
申请号：US17343905
申请日：2021-06-10
CPC分类号： C12N15/1037 , C07K16/00 , C07K16/2887 , C07K16/32 , C12N15/1034 , G01N33/6854 , C07K2319/30
摘要： Methods for display of recombinant whole immunoglobulins or immunoglobulin libraries on the surface of eukaryote host cells, including yeast and filamentous fungi, are described. The methods are useful for screening libraries of recombinant immunoglobulins in eukaryote host cells to identify immunoglobulins that are specific for an antigen of interest.
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3.发明公开公开(公告)号：US20240294899A1
公开(公告)日：2024-09-05
申请号：US18432527
申请日：2024-02-05
申请人： Iontas Limited
发明人： John MCCAFFERTY , Michael DYSON , Kothai PARTHIBAN
CPC分类号： C12N15/102 , C07K16/005 , C12N15/1037 , C12N15/907 , C07K2317/24 , C07K2317/622 , C07K2317/64 , C07K2319/03 , C07K2319/035 , C07K2319/036 , C12N2310/20 , C12N2800/30 , C12N2800/80
摘要： The invention relates to methods of producing eukaryotic cell libraries encoding a repertoire of binding molecules (“binders”), wherein the methods use a site-specific nuclease for targeted cleavage of cellular DNA to enhance site-specific integration of binder genes through endogenous cellular repair mechanisms. Populations of eukaryotic cells are produced in which a repertoire of genes encoding binders are integrated into a desired locus in cellular DNA (e.g., a genomic locus) allowing expression of the encoded binding molecule, thereby creating a population of cells expressing different binders.
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公开(公告)号：US12049712B2
公开(公告)日：2024-07-30
申请号：US17229859
申请日：2021-04-13
申请人： 10X Genomics, Inc.
IPC分类号： C40B30/04 , C07K14/74 , C12N15/10 , C12N15/11 , C12N15/85 , C12Q1/6804 , C12Q1/6806 , C12Q1/6818 , C12Q1/6827 , C12Q1/6881 , C40B50/06 , C40B70/00 , G01N33/50 , G01N33/53 , G01N33/532 , G01N33/543 , G01N33/548 , G01N33/569 , G01N33/58
CPC分类号： C40B30/04 , C07K14/70539 , C12N15/1037 , C12N15/1055 , C12N15/1065 , C12N15/1075 , C12N15/11 , C12N15/85 , C12Q1/6804 , C12Q1/6806 , C12Q1/6818 , C12Q1/6827 , C12Q1/6881 , G01N33/5032 , G01N33/505 , G01N33/5304 , G01N33/5306 , G01N33/5308 , G01N33/532 , G01N33/54306 , G01N33/54366 , G01N33/548 , G01N33/56977 , G01N33/58 , C12N2310/20 , C12N2320/10 , C12Q2537/164 , C12Q2563/179 , C12Q2563/185 , C12Q2565/1015 , C40B50/06 , C40B70/00
摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).
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公开(公告)号：US20240240259A1
公开(公告)日：2024-07-18
申请号：US18551356
申请日：2022-03-26
发明人： Sunil Sharma , Raffaella Soldi , John Altin , Jeffrey Trent
IPC分类号： C12Q1/6886 , A61K39/00 , C12N15/10
CPC分类号： C12Q1/6886 , A61K39/0011 , C12N15/1037 , A61K2039/53 , A61K2039/585 , C12Q2600/156
摘要： The present disclosure includes compounds and methods for treating a subject having a disease such as cancer. A treatment method includes administering to the patient a therapeutically effective amount of one or more peptides corresponding to a tumor neoantigen or administering to the patient a therapeutically effective amount of one or more oligonucleotides each having a nucleic acid sequence that encodes a peptide corresponding to a tumor neoantigen. The tumor neoantigens may be identified from patient-specific tumor mutations in the patient's tumor cells.
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公开(公告)号：US12037706B2
公开(公告)日：2024-07-16
申请号：US17718275
申请日：2022-04-11
发明人： Pierce Ogden , Gleb Kuznetsov , Shane Lofgren , Kathleen Nudel , Hoong Chuin Lim , Karen Duffy , Jeffrey Chang
CPC分类号： C40B40/08 , C07K16/005 , C07K16/18 , C07K17/02 , C12N15/1037 , C07K2317/55
摘要： Methods and systems for quantification of an abundance of one or more payloads (e.g. proteins) in a mixture (e.g. a complex mixture (e.g. in vivo)) using barcodes (e.g. peptide barcodes), binders (e.g. polypeptide binders), and binding agents (e.g. phage) are provided herein.
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公开(公告)号：US12037385B2
公开(公告)日：2024-07-16
申请号：US17346534
申请日：2021-06-14
申请人： Genentech, Inc.
CPC分类号： C07K16/18 , C07K14/4702 , C12N15/1037 , G01N33/68 , C07K2317/24 , C07K2317/30 , C07K2317/55 , C07K2317/56 , C07K2317/565 , C07K2317/92 , G01N2333/4703 , G01N2440/36
摘要： The invention provides anti-polyubiquitin antibodies and methods of using the same.
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公开(公告)号：US20240229297A1
公开(公告)日：2024-07-11
申请号：US18592473
申请日：2024-02-29
CPC分类号： C40B40/10 , C12N15/1037 , C12N15/81 , C40B40/08 , C40B50/06
摘要： Antibody libraries comprising a plurality of heavy chain variable domains and/or a plurality of light chain variable domains, which comprise complementary determining regions (CDRs) found in naturally-occurring human antibodies, and methods of making such antibody libraries. The antibody libraries are free of members that comprise one or more liabilities affecting one or more features of such members. Further, the antibody libraries comprise members having heavy chain and/or light chain CDRs not found in the same naturally-occurring human antibody.
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公开(公告)号：US20240207421A1
公开(公告)日：2024-06-27
申请号：US18254565
申请日：2021-12-03
发明人： Michael WHITNEY , Quyen NGUYEN
CPC分类号： A61K47/64 , A61K41/0061 , A61K41/0071 , A61K41/0076 , A61K41/008 , A61K49/0056 , C12N15/1037
摘要： The present disclosure provides nerve targeting peptides, including those comprising the amino acid sequence of any one of SEQ ID NOS: 1-710; conjugates comprising such nerve targeting peptides and a cargo molecule; compositions comprising said peptides and conjugates; and methods of said peptides and conjugates in surgical procedures and other applications.
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公开(公告)号：US12012593B2
公开(公告)日：2024-06-18
申请号：US17727975
申请日：2022-04-25
申请人： Miti Biosystems GmbH
发明人： Guntram Christiansen
CPC分类号： C12N15/1037 , C07K1/047 , C12N15/1082 , C40B40/02 , C40B40/10 , C40B50/06
摘要： The invention refers to a replicable genetic package displaying a peptide having at least one intramolecular cyclic bond between two heteroatoms of amino acid side chains, a method of preparing a replicable genetic package, a method of producing a library and a library of replicable genetic package.
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