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公开(公告)号：US20060099611A1
公开(公告)日：2006-05-11
申请号：US11156014
申请日：2005-06-17
申请人： Robert Irving , Peter Hudson , Huseyin Mustafa , Kim Wark , Matias Abregu
发明人： Robert Irving , Peter Hudson , Huseyin Mustafa , Kim Wark , Matias Abregu
CPC分类号： C40B40/02 , C12N15/102 , C12N15/1037
摘要： The present invention relates to the field of evolution of nucleic acids in vivo and provides methods and compositions for introducing diversity into gene products. The present invention allows generation of new sequences that have desirable properties by virtue of high frequency mutation events within a cell. The high frequency mutation of a polynucleotide sequence results in, the production of a large population of new sequence variants. Appropriate selection and/or screening permits identification and isolation of mutant forms of the polynucleotide sequence as well as products resulting from expression of the mutant sequences.
摘要翻译： 本发明涉及体内核酸进化领域，并提供了将多样性引入基因产物的方法和组合物。 本发明允许通过细胞内的高频突变事件产生具有期望特性的新序列。 多核苷酸序列的高频突变导致大量新序列变体的产生。 适当的选择和/或筛选允许鉴定和分离突变形式的多核苷酸序列以及由突变序列的表达产生的产物。
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公开(公告)号：US20070048774A1
公开(公告)日：2007-03-01
申请号：US11498974
申请日：2006-08-03
申请人： Gregory Coia , Peter Hudson , Peter Iliades , Robert Irving
发明人： Gregory Coia , Peter Hudson , Peter Iliades , Robert Irving
CPC分类号： C12N9/127 , C07K16/082 , C07K16/18 , C07K2317/622 , C12N15/102 , C12N15/1041 , C12N15/1058
摘要： Provided is a method for the mutation, synthesis and selection of a protein of interest, by first incubating a replicable RNA molecule encoding the protein with ribonucleoside triphosphate precursors of RNA and an RNA-directed RNA polymerase, such that the RNA-directed RNA polymerase replicates the RNA molecule but introduces mutations thereby generating a population of mutant RNA molecules. The mutant RNA molecules are then incubated with a translation system under conditions which result in the synthesis of a population of mutant proteins. After translation, the mutant proteins are linked to their encoding RNA molecules, and one or more mutant proteins of interest are selected.
摘要翻译： 提供了通过首先将编码蛋白质的可复制RNA分子与RNA的核糖核苷三磷酸前体和RNA指导的RNA聚合酶一起孵育来进行突变，合成和选择目的蛋白质的方法，使得RNA指导的RNA聚合酶复制 RNA分子引入突变，从而产生突变型RNA分子群体。 然后将突变体RNA分子与翻译系统在导致合成突变蛋白质群体的条件下孵育。 翻译后，突变蛋白与其编码RNA分子连接，并且选择一种或多种感兴趣的突变蛋白。
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公开(公告)号：US20070065431A1
公开(公告)日：2007-03-22
申请号：US11588966
申请日：2006-10-27
申请人： Gregory Coia , Maria Galanis , Peter Hudson , Robert Irving , Stewart Nuttall
发明人： Gregory Coia , Maria Galanis , Peter Hudson , Robert Irving , Stewart Nuttall
CPC分类号： C07K14/70521 , A61K38/00 , C07K16/3061 , C07K16/40 , C07K2317/21 , C07K2317/22 , C07K2319/00 , Y10S530/808 , Y10S530/81 , Y10S530/812
摘要： The present invention relates to binding moieties comprising at least one monomeric V-like domain (VLD) derived from a non-antibody ligand, the at least one monomeric V-like domain being characterised in that at least one CDR loop structure or part thereof is modified or replaced such that the solubility of the modified VLD is improved when compared with the unmodified VLD.
摘要翻译： 本发明涉及包含至少一种衍生自非抗体配体的单体V样结构域（VLD）的结合部分，所述至少一个单体V样结构域的特征在于至少一个CDR环结构或其部分是 修饰或替换，使得当与未修饰的VLD相比时，修饰的VLD的溶解度得到改善。
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4.发明授权公开(公告)号：US06883781B2
公开(公告)日：2005-04-26
申请号：US10318030
申请日：2002-12-13
申请人： Robert Irving
发明人： Robert Irving
CPC分类号： E04H17/265 , Y10T29/53839 , Y10T29/53843
摘要： The tool is adapted for extracting the sheared remnant of a sign post having holes and comprises a shaft having a top and bottom end. To secure the tool to the remnant a clamp is provided comprising a stationary member integral to the bottom end of the shaft and a moveable member pivotally connected to the stationary member. Fixed to the stationary member is a guide member having a plurality of outwardly projecting pins. The pins have a diameter and spaced relationship adapted to correspond to the diameter and spaced relationship of the holes in the sign post remnant. When the clamp is inserted into the sign post remnant the pins will enter and tightly engage the holes in the signpost remnant. Connected to the top end of the shaft is a sliding hammer for imparting extraction forces to the sheared sign post remnant.
摘要翻译： 该工具适于提取具有孔的标志杆的剪切残留物，并且包括具有顶端和底端的轴。 为了将工具固定到残余物上，提供夹具，其包括与轴的底端一体的固定构件和可枢转地连接到固定构件的可移动构件。 固定到固定构件的是具有多个向外突出的销的引导构件。 销具有直径和间隔的关系，其适于对应于标志残余物中的孔的直径和间隔关系。 当夹具插入标志残留物时，针脚将进入并紧紧接合路标残留物中的孔。 连接到轴的顶端是用于将剪切力施加到剪切标志残余物的滑动锤。
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公开(公告)号：US4248709A
公开(公告)日：1981-02-03
申请号：US73260
申请日：1979-09-07
申请人： Robert Irving
发明人： Robert Irving
CPC分类号： C02F11/121 , B01D19/02 , B01D33/11 , B01D35/18 , C02F11/00
摘要： The method of treating sewer scum including combustibles and debris includes the steps of collecting the scum from sewage, delivering it to a perforated filter drum, continuously rotating the drum upon an inclined longitudinal axis, applying heated air under pressure to the exterior of the drum along its length inwardly of the perforations, discharging the debris continuously and collecting the filtered combustibles. Apparatus for filtering said scum includes an elongated cylindrical perforated drum rotatable on an inclined axis receiving scum at its upper end and discharging debris at its lower end. A cylindrical shell sealingly encloses the perforated portion of the drum for providing a pressure chamber above the drum and a collection chamber below the drum. An elongated air channel along the interior of the shell delivers high pressure heated air to said pressure chamber and filtered scum accumulates in the collection chamber.
摘要翻译： 处理包括可燃物和碎屑在内的下水道浮渣的方法包括以下步骤：从污水中收集浮渣，将其输送到穿孔过滤鼓上，在倾斜纵向轴线上连续旋转滚筒，将压力下的加热空气加到滚筒外面 其长度在穿孔的内部，连续地排出碎片并收集过滤的可燃物。 用于过滤所述浮渣的装置包括可在其上端接收浮渣的倾斜轴上旋转的细长圆柱形穿孔鼓，并在其下端排放碎屑。 圆柱形壳体密封地包围滚筒的穿孔部分，用于在滚筒上方提供压力室，在滚筒下方设置收集室。 沿着壳体内部的细长空气通道将高压加热的空气传送到所述压力室，并且过滤的浮渣积聚在收集室中。
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公开(公告)号：US20090068372A1
公开(公告)日：2009-03-12
申请号：US11851709
申请日：2007-09-07
申请人： Robert Irving
发明人： Robert Irving
摘要： An apparatus for applying a wax seal to an article such as a wine bottle including a bottom tank formed of at least one upstanding wall and a bottom wall and having an open top end, the bottom tank being fitted with electrical heating means; an upper tank formed of at least one upstanding wall and having an open top end and a closed bottom wall, the upper tank being fitted with electrical heating means, the upper tank being connected at its closed bottom wall with the open top end of said bottom tank, a screen adjustably located inside the upper tank, a pump located in the bottom tank and extending into the upper tank for conveying molten wax from the bottom tank to the upper tank and an overflow opening in the upper tank.
摘要翻译： 一种用于将蜡密封件施加到诸如酒瓶的物品的装置，包括由至少一个直立的壁和底壁形成的底部容器，并且具有敞开的顶端，底部容器装有电加热装置; 由至少一个直立的壁形成并具有敞开的顶端和封闭的底壁的上部箱体，上部箱体装配有电加热装置，上部箱体在其封闭的底部壁处与所述底部的敞开的顶端连接 罐，可调节地位于上罐内的屏幕，位于底箱中并延伸到上罐中的泵，用于将熔融的蜡从底罐输送到上罐，并且在上罐中具有溢流开口。
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