公开(公告)号：US08600168B2

公开(公告)日：2013-12-03

申请号：US13253857

申请日：2011-10-05

Applicant: Simant Dube ,  Gang Sun ,  Lian-She Zhao

Inventor： Simant Dube ,  Gang Sun ,  Lian-She Zhao

IPC: G06K9/36 ,  G06K9/40 ,  G06T7/00

CPC classification number: G01N21/64 ,  G06T7/0012 ,  G06T7/74 ,  G06T2207/10064 ,  G06T2207/30004

Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.

Abstract translation: 处理与微流体装置的荧光发射有关的数据的方法。 该方法包括执行与微流体装置的第一图像相关联的自动聚焦处理，并且执行与微流体装置的第一图像相关联的自动曝光处理。 该方法还包括捕获微流体装置的多个图像。 多个图像与多个热循环相关联。 该方法还包括执行多个拍摄图像的图像分析以确定一系列光强度并执行该系列光强度的数据分析以提供一系列阈值变化。

公开(公告)号：US20080075322A1

公开(公告)日：2008-03-27

申请号：US11900926

申请日：2007-09-13

Applicant: Simant Dube ,  Gang Sun ,  Lian-She Zhao

Inventor： Simant Dube ,  Gang Sun ,  Lian-She Zhao

IPC: G06K9/00

CPC classification number: G06K9/00134

Abstract: A method of determining a plurality of positions associated with a plurality of reaction chambers of a microfluidic device includes a) providing a baseline image; b) providing a template image of a reaction chamber; and c) selecting a region of the baseline image. The method also includes d) performing a matching process including matching the template image to one or more portions of the region of the baseline image; e) determining a position of a first chamber; and f) predicting a position of a second chamber. The method further includes g) repeating steps c) through f) for subsequent chambers.

Abstract translation: 确定与微流体装置的多个反应室相关联的多个位置的方法包括：a）提供基线图像; b）提供反应室的模板图像; 和c）选择基线图像的区域。 该方法还包括：d）执行匹配过程，包括将模板图像与基线图像区域的一个或多个部分相匹配; e）确定第一室的位置; 以及f）预测第二室的位置。 该方法还包括g）重复步骤c）至f）以用于随后的室。

公开(公告)号：US20120245888A1

公开(公告)日：2012-09-27

申请号：US13253857

申请日：2011-10-05

Applicant: Simant Dube ,  Gang Sun ,  Lian-She Zhao

Inventor： Simant Dube ,  Gang Sun ,  Lian-She Zhao

IPC: G06F17/18 ,  G01J1/44

CPC classification number: G01N21/64 ,  G06T7/0012 ,  G06T7/74 ,  G06T2207/10064 ,  G06T2207/30004

Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.

Abstract translation: 处理与微流体装置的荧光发射有关的数据的方法。 该方法包括执行与微流体装置的第一图像相关联的自动聚焦处理，并且执行与微流体装置的第一图像相关联的自动曝光处理。 该方法还包括捕获微流体装置的多个图像。 多个图像与多个热循环相关联。 该方法还包括执行多个拍摄图像的图像分析以确定一系列光强度并执行该系列光强度的数据分析以提供一系列阈值变化。

公开(公告)号：US20080075380A1

公开(公告)日：2008-03-27

申请号：US11900927

申请日：2007-09-13

Applicant: Simant Dube ,  Gang Sun ,  Lian-She Zhao

Inventor： Simant Dube ,  Gang Sun ,  Lian-She Zhao

IPC: G06K9/40

CPC classification number: G01N21/64 ,  G06T7/0012 ,  G06T7/74 ,  G06T2207/10064 ,  G06T2207/30004

Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.

Abstract translation: 处理与微流体装置的荧光发射有关的数据的方法。 该方法包括执行与微流体装置的第一图像相关联的自动聚焦处理，并且执行与微流体装置的第一图像相关联的自动曝光处理。 该方法还包括捕获微流体装置的多个图像。 多个图像与多个热循环相关联。 该方法还包括执行多个拍摄图像的图像分析以确定一系列光强度并执行该系列光强度的数据分析以提供一系列阈值变化。

公开(公告)号：US6019896A

公开(公告)日：2000-02-01

申请号：US36767

申请日：1998-03-06

Applicant: Elaine S. Mansfield ,  Lian-She Zhao ,  Marina Vainer ,  Curtis R. Kautzer

Inventor： Elaine S. Mansfield ,  Lian-She Zhao ,  Marina Vainer ,  Curtis R. Kautzer

IPC: G01N33/50 ,  B01D15/08 ,  C12N15/09 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68 ,  G01N27/447 ,  G01N30/86 ,  G01N30/00

CPC classification number: G01N30/8665 ,  C12N15/101 ,  C12Q1/68 ,  C12Q1/6827 ,  G01N27/44717 ,  G01N30/8634 ,  G01N2201/129 ,  G01N30/8631 ,  G01N30/8658

Abstract: A method for performing separation assays of biochemical samples includes computing a quality metric based on peak data produced during the separation run. The quality metric is the basis for selecting a subsequent step in the assay, including whether to re-run the separation when the quality metric indicates a low quality separation run. In a preferred embodiment, the quality metric is computed based on a peak resolution metric indicative of the peak resolution of the sample peaks in the data and a signal-to-noise ratio of the data. When a co-migrating standard is included in the separation run, the quality metric is further based on the degree of migration linearity of the reference peaks produced by the standard. The method was reduced to practice in separations to size and sort DNA fragments in high-throughput capillary array electrophoresis separations.

Abstract translation: 用于进行生物化学样品的分离测定的方法包括基于在分离运行期间产生的峰值数据来计算质量度量。 质量度量是选择测定中的后续步骤的基础，包括当质量度量指示低质量分离运行时是否重新运行分离。 在优选实施例中，基于指示数据中的样本峰值的峰值分辨率的峰值分辨率度量和数据的信噪比来计算质量度量。 当分离运行中包含共迁移标准时，质量度量还基于由标准产生的参考峰的迁移线性度。 在高通量毛细管阵列电泳分离中，将该方法减少到分离中的大小和分类DNA片段。

公开(公告)号：US08055034B2

公开(公告)日：2011-11-08

申请号：US11900927

申请日：2007-09-13

Applicant: Simant Dube ,  Gang Sun ,  Lian-She Zhao

Inventor： Simant Dube ,  Gang Sun ,  Lian-She Zhao

IPC: G06K9/36

CPC classification number: G01N21/64 ,  G06T7/0012 ,  G06T7/74 ,  G06T2207/10064 ,  G06T2207/30004

Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.

Abstract translation: 处理与微流体装置的荧光发射有关的数据的方法。 该方法包括执行与微流体装置的第一图像相关联的自动聚焦处理，并且执行与微流体装置的第一图像相关联的自动曝光处理。 该方法还包括捕获微流体装置的多个图像。 多个图像与多个热循环相关联。 该方法还包括执行多个拍摄图像的图像分析以确定一系列光强度并执行该系列光强度的数据分析以提供一系列阈值变化。

公开(公告)号：US08050516B2

公开(公告)日：2011-11-01

申请号：US11900926

申请日：2007-09-13

Applicant: Simant Dube ,  Gang Sun ,  Lian-She Zhao

Inventor： Simant Dube ,  Gang Sun ,  Lian-She Zhao

IPC: G06K9/36

CPC classification number: G06K9/00134

Abstract: A method of determining a plurality of positions associated with a plurality of reaction chambers of a microfluidic device includes a) providing a baseline image; b) providing a template image of a reaction chamber; and c) selecting a region of the baseline image. The method also includes d) performing a matching process including matching the template image to one or more portions of the region of the baseline image; e) determining a position of a first chamber; and f) predicting a position of a second chamber. The method further includes g) repeating steps c) through f) for subsequent chambers.

Abstract translation: 确定与微流体装置的多个反应室相关联的多个位置的方法包括：a）提供基线图像; b）提供反应室的模板图像; 和c）选择基线图像的区域。 该方法还包括：d）执行匹配过程，包括将模板图像与基线图像区域的一个或多个部分相匹配; e）确定第一室的位置; 以及f）预测第二室的位置。 该方法还包括g）重复步骤c）至f）以用于随后的室。