公开(公告)号：US11275072B2

公开(公告)日：2022-03-15

申请号：US15731004

申请日：2017-04-05

申请人： Jonathan C. Evans ,  Theodore W. Selby ,  Marta Manning

发明人： Jonathan C. Evans ,  Theodore W. Selby ,  Marta Manning

IPC分类号： G01N33/28 ,  C10M109/00 ,  C10M121/04 ,  C10M169/02 ,  C10M113/06 ,  G01N33/00 ,  G01N21/35 ,  C10N40/25

摘要： Effects of Oxygen on grease or another organic paste product can be evaluated with a modified version of the ASTM D942 test methodology for oxidation stability of lubricating greases or other organic paste products by employment of an oxidation pressure vessel, in which at least one of (A) a very small sample of the grease or other organic paste product is deployed for testing within the oxidation pressure vessel such that the sample has an enhanced surface area to mass ratio, and (B) a temperature other than about 99° C. is employed during the testing. In general, the testing is carried out under oxidation pressure conditions. The sample may be evaluated with respect to Oxygen uptake. Additional technique(s) directed to oxidation and/or other properties of the sample may be carried out before to after any Oxygen uptake evaluation, for example, FTIR analysis and/or ATR-FTIR analysis.

公开(公告)号：US20180003692A1

公开(公告)日：2018-01-04

申请号：US15731024

申请日：2017-04-07

申请人： Jonathan C. Evans ,  Theodore W. Selby ,  Marta Manning ,  Derrick D. Hilliker ,  Michael A. Habitz

发明人： Jonathan C. Evans ,  Theodore W. Selby ,  Marta Manning ,  Derrick D. Hilliker ,  Michael A. Habitz

IPC分类号： G01N33/28 ,  C10M105/00 ,  C10M121/04 ,  C10M105/32 ,  C10M113/06

CPC分类号： G01N33/2888 ,  C10M105/00 ,  C10M105/32 ,  C10M113/06 ,  C10M117/02 ,  C10M121/04 ,  C10M2201/056 ,  C10M2203/003 ,  C10M2203/10 ,  C10M2207/2805 ,  C10M2227/09 ,  C10N2230/06 ,  C10N2250/10

摘要： Grease wear test device includes a body in a form of a collar having an outer surface, a first side and an opposing second side, which are open to form a hollow, open channel through the body, which has a center axis, into which opposing vee blocks can be inserted; and perpendicular to the center axis, a pair of opposing holes through and open to the outer surface of the body and the cylindrical inside wall, through which a cylindrical test journal (falex pin) can be inserted for contact in general with opposing vee-shaped channels of the inserted, opposing vee blocks, and for rotation during testing. The device can be used to modify a falex pin and vee block device, to receive and contain a small sample of grease or another organic paste product for testing such as a modified version of an ASTM D2670 test method for measuring wear properties of fluid lubricants (falex pin and vee block method).

公开(公告)号：US20050069957A1

公开(公告)日：2005-03-31

申请号：US10924235

申请日：2004-08-23

申请人： Wilfredo Colon ,  Marta Manning

发明人： Wilfredo Colon ,  Marta Manning

IPC分类号： G01N33/68 ,  G01N33/53 ,  C12Q1/68 ,  G01N33/543

CPC分类号： G01N33/6803 ,  G01N2500/00

摘要： The present invention provides a fast and efficient means for identifying kinetically stable proteins. As used herein the term “kinetically stable protein” means a protein that is trapped in a specific conformation due to an unusually high unfolding barrier that results in very slow unfolding rates. The present inventors are the first to discover the existence of a correlation between kinetic stability and SDS-induced denaturation. Thus, the invention provides methods for identifying kinetically stable proteins comprising the step of testing the proteins for resistance to denaturation by SDS. In one embodiment, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) is one simple method for quickly identifying and selecting kinetically stable proteins.

摘要翻译： 本发明提供用于鉴定动力学稳定蛋白质的快速和有效的方法。 如本文所用，术语“动力学稳定蛋白”是指由于非常高的展开屏障导致非常缓慢的展开速率而被捕获在特定构象中的蛋白质。 本发明人首先发现动力学稳定性与SDS诱导的变性之间存在相关性。 因此，本发明提供了用于鉴定动力学稳定蛋白质的方法，其包括测试蛋白质以抵抗SDS变性的步骤。 在一个实施方案中，SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）是快速鉴定和选择动力学稳定蛋白质的简单方法。

公开(公告)号：US07217348B2

公开(公告)日：2007-05-15

申请号：US11226072

申请日：2005-09-14

申请人： Wilfredo Colón ,  Marta Manning

发明人： Wilfredo Colón ,  Marta Manning

IPC分类号： B01D57/02

CPC分类号： G01N33/6803 ,  G01N2500/00

摘要： The present invention provides a fast and efficient means for identifying kinetically stable proteins. As used herein the term “kinetically stable protein” means a protein that is trapped in a specific conformation due to an unusually high unfolding barrier that results in very slow unfolding rates. The present inventors are the first to discover the existence of a correlation between kinetic stability and SDS-induced denaturation. Thus, the invention provides methods for identifying kinetically stable proteins comprising the step of testing the proteins for resistance to denaturation by SDS. In one embodiment, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) is one simple method for quickly identifying and selecting kinetically stable proteins. In another embodiment a two-dimensional SDS-PAGE provides a high throughput method for quickly identifying kinetically stable proteins in a sample.

摘要翻译： 本发明提供用于鉴定动力学稳定蛋白质的快速和有效的方法。 如本文所用，术语“动力学稳定蛋白”是指由于非常高的展开屏障导致非常缓慢的展开速率而被捕获在特定构象中的蛋白质。 本发明人首先发现动力学稳定性与SDS诱导的变性之间存在相关性。 因此，本发明提供了用于鉴定动力学稳定蛋白质的方法，其包括测试蛋白质以抵抗SDS变性的步骤。 在一个实施方案中，SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）是快速鉴定和选择动力学稳定蛋白质的简单方法。 在另一个实施方案中，二维SDS-PAGE提供用于快速鉴定样品中的动力学稳定蛋白质的高通量方法。

公开(公告)号：US20170284994A1

公开(公告)日：2017-10-05

申请号：US15731004

申请日：2017-04-05

申请人： Jonathan C. Evans ,  Theodore W. Selby ,  Marta Manning

发明人： Jonathan C. Evans ,  Theodore W. Selby ,  Marta Manning

IPC分类号： G01N33/28 ,  C10M113/06 ,  C10M169/02 ,  C10M109/00 ,  C10M121/04

CPC分类号： G01N33/2805 ,  C10M109/00 ,  C10M113/06 ,  C10M121/04 ,  C10M169/02 ,  C10N2240/10 ,  G01N21/35 ,  G01N33/2888 ,  G01N2021/3595

摘要： Effects of Oxygen on grease or another organic paste product can be evaluated with a modified version of the ASTM D942 test methodology for oxidation stability of lubricating greases or other organic paste products by employment of an oxidation pressure vessel, in which at least one of (A) a very small sample of the grease or other organic paste product is deployed for testing within the oxidation pressure vessel such that the sample has an enhanced surface area to mass ratio, and (B) a temperature other than about 99° C. is employed during the testing. In general, the testing is carried out under oxidation pressure conditions. The sample may be evaluated with respect to Oxygen uptake. Additional technique(s) directed to oxidation and/or other properties of the sample may be carried out before to after any Oxygen uptake evaluation, for example, FTIR analysis and/or ATR-FTIR analysis.

公开(公告)号：US20060099647A1

公开(公告)日：2006-05-11

申请号：US11226072

申请日：2005-09-14

申请人： Wilfredo Colon ,  Marta Manning

发明人： Wilfredo Colon ,  Marta Manning

IPC分类号： C40B40/10

CPC分类号： G01N33/6803 ,  G01N2500/00

摘要： The present invention provides a fast and efficient means for identifying kinetically stable proteins. As used herein the term “kinetically stable protein” means a protein that is trapped in a specific conformation due to an unusually high unfolding barrier that results in very slow unfolding rates. The present inventors are the first to discover the existence of a correlation between kinetic stability and SDS-induced denaturation. Thus, the invention provides methods for identifying kinetically stable proteins comprising the step of testing the proteins for resistance to denaturation by SDS. In one embodiment, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) is one simple method for quickly identifying and selecting kinetically stable proteins. In another embodiment a two-dimensional SDS-PAGE provides a high throughput method for quickly identifying kinetically stable proteins in a sample.

摘要翻译： 本发明提供用于鉴定动力学稳定蛋白质的快速和有效的方法。 如本文所用，术语“动力学稳定蛋白”是指由于非常高的展开屏障导致非常缓慢的展开速率而被捕获在特定构象中的蛋白质。 本发明人首先发现动力学稳定性与SDS诱导的变性之间存在相关性。 因此，本发明提供了用于鉴定动力学稳定蛋白质的方法，其包括测试蛋白质以抵抗SDS变性的步骤。 在一个实施方案中，SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）是快速鉴定和选择动力学稳定蛋白质的简单方法。 在另一个实施方案中，二维SDS-PAGE提供用于快速鉴定样品中的动力学稳定蛋白质的高通量方法。

公开(公告)号：US10302619B2

公开(公告)日：2019-05-28

申请号：US15731024

申请日：2017-04-07

申请人： Jonathan C. Evans ,  Theodore W. Selby ,  Marta Manning ,  Derrick D. Hilliker ,  Michael A. Habitz

发明人： Jonathan C. Evans ,  Theodore W. Selby ,  Marta Manning ,  Derrick D. Hilliker ,  Michael A. Habitz

IPC分类号： G01N33/28 ,  C10M117/02 ,  C10M121/04 ,  C10M113/06 ,  C10M105/32 ,  C10M105/00

摘要： Grease wear test device includes a body in a form of a collar having an outer surface, a first side and an opposing second side, which are open to form a hollow, open channel through the body, which has a center axis, into which opposing vee blocks can be inserted; and perpendicular to the center axis, a pair of opposing holes through and open to the outer surface of the body and the cylindrical inside wall, through which a cylindrical test journal (falex pin) can be inserted for contact in general with opposing vee-shaped channels of the inserted, opposing vee blocks, and for rotation during testing. The device can be used to modify a falex pin and vee block device, to receive and contain a small sample of grease or another organic paste product for testing such as a modified version of an ASTM D2670 test method for measuring wear properties of fluid lubricants (falex pin and vee block method).

公开(公告)号：US10663449B2

公开(公告)日：2020-05-26

申请号：US16350334

申请日：2018-11-01

申请人： Jonathan C. Evans ,  Theodore W. Selby ,  Marta Manning ,  Gregory C. Miiller ,  William John VanBergen

发明人： Jonathan C. Evans ,  Theodore W. Selby ,  Marta Manning ,  Gregory C. Miiller ,  William John VanBergen

IPC分类号： G01N33/28 ,  B01J10/02

摘要： Elevated temperature liquid testing apparatus and methodology in which a thin film of test liquid and a reactant/control gas are provided about the top of a depositor member that is surrounded by a special mantle, for example, a substantially cylindrically walled glass mantle. As an oxidative engine oil test, it may mimic turbocharger conditions of a modern internal combustion engine. For example, employing moist air, the apparatus can test a thin film of engine oil for oxidation deposits at a predetermined temperature, say, 285° C., 290° C., or cycled between 285° C. or 290° C. and 320° C. or 330° C.

公开(公告)号：US20190145948A1

公开(公告)日：2019-05-16

申请号：US16350334

申请日：2018-11-01

申请人： Jonathan C. Evans ,  Theodore W. Selby ,  Marta Manning ,  Gregory C. Miiller ,  William John VanBergen

发明人： Jonathan C. Evans ,  Theodore W. Selby ,  Marta Manning ,  Gregory C. Miiller ,  William John VanBergen

IPC分类号： G01N33/28

CPC分类号： G01N33/2805 ,  B01J10/02 ,  G01N33/2817

摘要： Elevated temperature liquid testing apparatus and methodology in which a thin film of test liquid and a reactant/control gas are provided about the top of a depositor member that is surrounded by a special mantle, for example, a substantially cylindrically walled glass mantle. As an oxidative engine oil test, it may mimic turbocharger conditions of a modern internal combustion engine. For example, employing moist air, the apparatus can test a thin film of engine oil for oxidation deposits at a predetermined temperature, say, 285° C., 290° C., or cycled between 285° C. or 290° C. and 320° C. or 330° C.

公开(公告)号：US07393443B2

公开(公告)日：2008-07-01

申请号：US10924235

申请日：2004-08-23

申请人： Wilfredo Colón ,  Marta Manning

发明人： Wilfredo Colón ,  Marta Manning

IPC分类号： B01D59/42 ,  G01N31/00

CPC分类号： G01N33/6803 ,  G01N2500/00

摘要： The present invention provides a fast and efficient means for identifying kinetically stable proteins. As used herein the term “kinetically stable protein” means a protein that is trapped in a specific conformation due to an unusually high unfolding barrier that results in very slow unfolding rates. The present inventors are the first to discover the existence of a correlation between kinetic stability and SDS-induced denaturation. Thus, the invention provides methods for identifying kinetically stable proteins comprising the step of testing the proteins for resistance to denaturation by SDS. In one embodiment, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) is one simple method for quickly identifying and selecting kinetically stable proteins.

摘要翻译： 本发明提供用于鉴定动力学稳定蛋白质的快速和有效的方法。 如本文所用，术语“动力学稳定蛋白”是指由于非常高的展开屏障导致非常缓慢的展开速率而被捕获在特定构象中的蛋白质。 本发明人首先发现动力学稳定性与SDS诱导的变性之间存在相关性。 因此，本发明提供了用于鉴定动力学稳定蛋白质的方法，其包括测试蛋白质以抵抗SDS变性的步骤。 在一个实施方案中，SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）是快速鉴定和选择动力学稳定蛋白质的简单方法。