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公开(公告)号:US08946507B2
公开(公告)日:2015-02-03
申请号:US12819702
申请日:2010-06-21
申请人: Timothy Robert Hawkes , Glynn Mitchell , Stephen Thomas Hadfield , Paul Anthony Thompson , Russell Viner , Yan Zhang
发明人: Timothy Robert Hawkes , Glynn Mitchell , Stephen Thomas Hadfield , Paul Anthony Thompson , Russell Viner , Yan Zhang
CPC分类号: C12N9/0024 , C12N9/18 , C12N15/8287 , C12N15/8289 , C12N15/829 , C12P13/04 , C12P41/007
摘要: A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male or female reproductive structures, characterised in that (i) the non-phytotoxic substance is D-phosphinothricin and (ii) the enzyme is a D-amino acid oxidase.
摘要翻译: 一种产生雄性或雌性不育植物的方法,包括以编码与非植物毒性物质反应产生植物毒性物质的至少一种酶的多核苷酸转化植物材料并将由此转化的材料再生成植物的步骤,其中 所述非植物毒性物质直到雄性或雌性配子形成和/或成熟时被施用于植物,使得非植物毒性物质提供了用于选择性地防止形成或以其它方式形成的植物毒性物质 所述配子是非功能性的,其中所述酶优先在雄性或雌性生殖结构中表达,其特征在于(i)非植物毒性物质是D-膦丝菌素,和(ii)所述酶是D-氨基酸氧化酶。
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公开(公告)号:US20100293666A1
公开(公告)日:2010-11-18
申请号:US12819702
申请日:2010-06-21
申请人: Timothy Robert Hawkes , Glynn Mitchell , Stephen Thomas Hadfield , Paul Anthony Thompson , Russell Viner , Yan Zhang
发明人: Timothy Robert Hawkes , Glynn Mitchell , Stephen Thomas Hadfield , Paul Anthony Thompson , Russell Viner , Yan Zhang
CPC分类号: C12N9/0024 , C12N9/18 , C12N15/8287 , C12N15/8289 , C12N15/829 , C12P13/04 , C12P41/007
摘要: A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male or female reproductive structures, characterised in that (i) the non-phytotoxic substance is D-phosphinothricin and (ii) the enzyme is a D-amino acid oxidase.
摘要翻译: 一种产生雄性或雌性不育植物的方法,包括以编码与非植物毒性物质反应产生植物毒性物质的至少一种酶的多核苷酸转化植物材料并将由此转化的材料再生成植物的步骤,其中 所述非植物毒性物质直到雄性或雌性配子形成和/或成熟时被施用于植物,使得非植物毒性物质提供了用于选择性地防止形成或以其它方式形成的植物毒性物质 所述配子是非功能性的,其中所述酶优先在雄性或雌性生殖结构中表达,其特征在于(i)非植物毒性物质是D-膦丝菌素,和(ii)所述酶是D-氨基酸氧化酶。
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公开(公告)号:US20080250535A1
公开(公告)日:2008-10-09
申请号:US12111364
申请日:2008-04-29
申请人: Timothy Robert Hawkes , Glynn Mitchell , Stephen Thomas Hadfield , Paul Anthony Thompson , Russell Viner , Yan Zhang
发明人: Timothy Robert Hawkes , Glynn Mitchell , Stephen Thomas Hadfield , Paul Anthony Thompson , Russell Viner , Yan Zhang
CPC分类号: C12N9/0024 , C12N9/18 , C12N15/8287 , C12N15/8289 , C12N15/829 , C12P13/04 , C12P41/007
摘要: A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male or female reproductive structures, characterised in that (i) the non-phytotoxic substance is a D-alpha amino acid, and (ii) the enzyme is a D-amino acid oxidase.
摘要翻译: 一种产生雄性或雌性不育植物的方法,包括以编码与非植物毒性物质反应产生植物毒性物质的至少一种酶的多核苷酸转化植物材料并将由此转化的材料再生成植物的步骤,其中 所述非植物毒性物质直到雄性或雌性配子形成和/或成熟时被施用于植物,使得非植物毒性物质提供了用于选择性地防止形成或以其它方式形成的植物毒性物质 所述配子是非功能性的,其中所述酶优先在雄性或雌性生殖结构中表达,其特征在于(i)非植物毒性物质是D-α氨基酸,和(ii)所述酶是D- 氨基酸氧化酶。
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公开(公告)号:US08642836B2
公开(公告)日:2014-02-04
申请号:US12111364
申请日:2008-04-29
申请人: Timothy Robert Hawkes , Glynn Mitchell , Stephen Thomas Hadfield , Paul Anthony Thompson , Russell Viner , Yan Zhang
发明人: Timothy Robert Hawkes , Glynn Mitchell , Stephen Thomas Hadfield , Paul Anthony Thompson , Russell Viner , Yan Zhang
CPC分类号: C12N9/0024 , C12N9/18 , C12N15/8287 , C12N15/8289 , C12N15/829 , C12P13/04 , C12P41/007
摘要: A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male or female reproductive structures, characterized in that (i) the non-phytotoxic substance is a D-alpha amino acid, and (ii) the enzyme is a D-amino acid oxidase.
摘要翻译: 一种产生雄性或雌性不育植物的方法,包括以编码与非植物毒性物质反应产生植物毒性物质的至少一种酶的多核苷酸转化植物材料并将由此转化的材料再生成植物的步骤,其中 所述非植物毒性物质直到雄性或雌性配子形成和/或成熟时被施用于植物,使得非植物毒性物质提供了用于选择性地防止形成或以其它方式形成的植物毒性物质 所述配子是非功能性的,其中所述酶优先在雄性或雌性生殖结构中表达,其特征在于(i)非植物毒性物质是D-α氨基酸,和(ii)所述酶是D- 氨基酸氧化酶。
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公开(公告)号:US07939709B2
公开(公告)日:2011-05-10
申请号:US10504784
申请日:2003-02-14
申请人: Timothy Robert Hawkes , Glynn Mitchell , Stephen Thomas Hadfield , Paul Anthony Thompson , Russell Viner , Yan Zhang
发明人: Timothy Robert Hawkes , Glynn Mitchell , Stephen Thomas Hadfield , Paul Anthony Thompson , Russell Viner , Yan Zhang
CPC分类号: C12N9/0024 , C12N9/18 , C12N15/8287 , C12N15/8289 , C12N15/829 , C12P13/04 , C12P41/007
摘要: A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male or female reproductive structures, characterised in that (i) the non-phytotoxic substance is a D-alpha amino acid, and (ii) the enzyme is a D-amino acid oxidase.
摘要翻译: 一种产生雄性或雌性不育植物的方法,包括以编码与非植物毒性物质反应产生植物毒性物质的至少一种酶的多核苷酸转化植物材料并将由此转化的材料再生成植物的步骤,其中 所述非植物毒性物质直到雄性或雌性配子形成和/或成熟时被施用于植物,使得非植物毒性物质提供了用于选择性地防止形成或以其它方式形成的植物毒性物质 所述配子是非功能性的,其中所述酶优先在雄性或雌性生殖结构中表达,其特征在于(i)非植物毒性物质是D-α氨基酸,和(ii)所述酶是D- 氨基酸氧化酶。
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公开(公告)号:US20100291643A1
公开(公告)日:2010-11-18
申请号:US12818548
申请日:2010-06-18
申请人: Timothy Robert Hawkes , Glynn Mitchell , Stephen Thomas Hadfield , Paul Anthony Thompson , Russell Viner , Yan Zhang
发明人: Timothy Robert Hawkes , Glynn Mitchell , Stephen Thomas Hadfield , Paul Anthony Thompson , Russell Viner , Yan Zhang
IPC分类号: C12P9/00
CPC分类号: C12N9/0024 , C12N9/18 , C12N15/8287 , C12N15/8289 , C12N15/829 , C12P13/04 , C12P41/007
摘要: A method of producing enantimoerically pure D-Phosphinothricin (D-PPT) by providing a mixture of D and L PPT, N-acetylating L-PPT and then recovering and purifying D-PPT.
摘要翻译: 通过提供D和L PPT,N-乙酰化L-PPT的混合物,然后回收和纯化D-PPT,制备具有同义词的纯D - 膦丝菌素(D-PPT)的方法。
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