公开(公告)号：US11414688B2

公开(公告)日：2022-08-16

申请号：US16717375

申请日：2019-12-17

Applicant: 10X GENOMICS, INC.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Zahra Kamila Belhocine

IPC: C40B20/04 ,  C12P19/34 ,  C12Q1/6806

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

公开(公告)号：US20200165603A1

公开(公告)日：2020-05-28

申请号：US16419461

申请日：2019-05-22

Applicant: 10X Genomics, Inc.

Inventor： Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Katherine Pfeiffer ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6869 ,  C12Q1/6806

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US20200024596A1

公开(公告)日：2020-01-23

申请号：US16419428

申请日：2019-05-22

Applicant: 10X Genomics, Inc.

Inventor： Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Katherine Pfeiffer ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US20250137025A1

公开(公告)日：2025-05-01

申请号：US18940636

申请日：2024-11-07

Applicant: 10x Genomics, Inc.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Zahra Kamila Belhocine

IPC: C12P19/34 ,  C12Q1/6806 ,  C40B20/04

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

公开(公告)号：US12264316B2

公开(公告)日：2025-04-01

申请号：US16419428

申请日：2019-05-22

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Katherine Pfeiffer ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US11193122B2

公开(公告)日：2021-12-07

申请号：US17208369

申请日：2021-03-22

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US20210222158A1

公开(公告)日：2021-07-22

申请号：US17208369

申请日：2021-03-22

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Katherine Pfeiffer ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US10557158B2

公开(公告)日：2020-02-11

申请号：US16228362

申请日：2018-12-20

Applicant: 10X GENOMICS, INC.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Kamila Belhocine

IPC: C12P19/34 ,  C40B20/04 ,  C12Q1/6806

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

公开(公告)号：US20190169666A1

公开(公告)日：2019-06-06

申请号：US16228362

申请日：2018-12-20

Applicant: 10X GENOMICS, INC.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Kamila Belhocine

IPC: C12P19/34 ,  C12Q1/6806 ,  C40B20/04

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

公开(公告)号：US20250146181A1

公开(公告)日：2025-05-08

申请号：US19016998

申请日：2025-01-10

Applicant: 10x Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Katherine Pfeiffer ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C40B30/04 ,  C07K14/74 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C40B50/06 ,  C40B70/00 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532 ,  G01N33/543 ,  G01N33/548 ,  G01N33/569 ,  G01N33/58

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.