公开(公告)号：US10221436B2

公开(公告)日：2019-03-05

申请号：US14990276

申请日：2016-01-07

Applicant: 10X Genomics, Inc.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Kamila Belhocine

IPC: C40B20/04 ,  C12P19/34 ,  C12Q1/6806

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

公开(公告)号：US20240191271A1

公开(公告)日：2024-06-13

申请号：US18388735

申请日：2023-11-10

Applicant: 10X GENOMICS, INC.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Zahra Kamila Belhocine

IPC: C12P19/34 ,  C12Q1/6806 ,  C40B20/04

CPC classification number: C12P19/34 ,  C12Q1/6806 ,  C40B20/04 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00722

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

公开(公告)号：US20160257984A1

公开(公告)日：2016-09-08

申请号：US14990276

申请日：2016-01-07

Applicant: 10X Genomics, Inc.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Kamila Belhocine

IPC: C12P19/34

CPC classification number: C12P19/34 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00722 ,  C12Q1/6806 ,  C40B20/04 ,  C12Q2525/121 ,  C12Q2525/185 ,  C12Q2525/191 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2535/122 ,  C12Q2563/149

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

Abstract translation: 本公开提供了制备测序文库的方法，包括提供模板核酸序列，dNTP，dUTP，引物，聚合酶，dUTP切割酶和包含寡核苷酸衔接子序列片段的多个珠粒的步骤; 用聚合酶，dNTP，dUTP和随机六聚体扩增模板核酸，以提供包括偶尔的dUTP的互补核酸序列; 并用dUTP切割酶切除掺入的dUTP以在互补核酸序列中提供缺口以提供测序文库。

公开(公告)号：US20160024558A1

公开(公告)日：2016-01-28

申请号：US14806471

申请日：2015-07-22

Applicant: 10X Genomics, Inc.

Inventor： Paul Hardenbol ,  Keith Bjornson

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6848 ,  C12Q2521/531

Abstract: Compositions, systems and methods employing nucleic acid binding proteins for use in the regulation and/or modulation of nucleic acid based reactions, including transcription, translation, modification, digestion, and hybridization reactions. Such compositions are employed in controlling a variety of different reaction types involving nucleic acids.

Abstract translation: 使用核酸结合蛋白的组合物，系统和方法用于调节和/或调节基于核酸的反应，包括转录，翻译，修饰，消化和杂交反应。 这样的组合物用于控制涉及核酸的各种不同的反应类型。

公开(公告)号：US11414688B2

公开(公告)日：2022-08-16

申请号：US16717375

申请日：2019-12-17

Applicant: 10X GENOMICS, INC.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Zahra Kamila Belhocine

IPC: C40B20/04 ,  C12P19/34 ,  C12Q1/6806

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

公开(公告)号：US20250137025A1

公开(公告)日：2025-05-01

申请号：US18940636

申请日：2024-11-07

Applicant: 10x Genomics, Inc.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Zahra Kamila Belhocine

IPC: C12P19/34 ,  C12Q1/6806 ,  C40B20/04

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

公开(公告)号：US10557158B2

公开(公告)日：2020-02-11

申请号：US16228362

申请日：2018-12-20

Applicant: 10X GENOMICS, INC.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Kamila Belhocine

IPC: C12P19/34 ,  C40B20/04 ,  C12Q1/6806

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

公开(公告)号：US20190169666A1

公开(公告)日：2019-06-06

申请号：US16228362

申请日：2018-12-20

Applicant: 10X GENOMICS, INC.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Kamila Belhocine

IPC: C12P19/34 ,  C12Q1/6806 ,  C40B20/04

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.