公开(公告)号：US20230015226A1

公开(公告)日：2023-01-19

申请号：US17852250

申请日：2022-06-28

Applicant: 10x Genomics, Inc.

Inventor： Hong CHEN ,  Evan R. DAUGHARTHY ,  Zhao ZHAO

IPC: C12Q1/6841

Abstract: The present application provides methods, compositions, and kits for analyzing a biological sample embedded in a three-dimensional polymerized matrix using a primer-exchange reaction (PER). In some embodiments, the methods comprise contacting the sample with a nucleic acid molecule that directly or indirectly binds to an analyte in the sample and immobilizing the nucleic acid molecule in the matrix, wherein the nucleic acid molecule comprises a free 3′ priming region for initiation of PER. In some embodiments, the methods enable sensitive detection of the identity and relative position of analytes in the sample.

公开(公告)号：US20230323437A1

公开(公告)日：2023-10-12

申请号：US17816991

申请日：2022-08-02

Applicant: 10x Genomics, Inc.

Inventor： Hong CHEN ,  Evan R. DAUGHARTHY ,  Shoshoni DROZ ,  Milena LAZOVA ,  Zhao ZHAO

IPC: C12Q1/6806 ,  C12Q1/6841

CPC classification number: C12Q1/6806 ,  C12Q1/6841

Abstract: The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein address one or more issues associated with the stability and/or size of nucleic acid structures such as rolling circle amplification products in the biological sample.