公开(公告)号：US20230015226A1

公开(公告)日：2023-01-19

申请号：US17852250

申请日：2022-06-28

Applicant: 10x Genomics, Inc.

Inventor： Hong CHEN ,  Evan R. DAUGHARTHY ,  Zhao ZHAO

IPC: C12Q1/6841

Abstract: The present application provides methods, compositions, and kits for analyzing a biological sample embedded in a three-dimensional polymerized matrix using a primer-exchange reaction (PER). In some embodiments, the methods comprise contacting the sample with a nucleic acid molecule that directly or indirectly binds to an analyte in the sample and immobilizing the nucleic acid molecule in the matrix, wherein the nucleic acid molecule comprises a free 3′ priming region for initiation of PER. In some embodiments, the methods enable sensitive detection of the identity and relative position of analytes in the sample.

公开(公告)号：US20230323437A1

公开(公告)日：2023-10-12

申请号：US17816991

申请日：2022-08-02

Applicant: 10x Genomics, Inc.

Inventor： Hong CHEN ,  Evan R. DAUGHARTHY ,  Shoshoni DROZ ,  Milena LAZOVA ,  Zhao ZHAO

IPC: C12Q1/6806 ,  C12Q1/6841

CPC classification number: C12Q1/6806 ,  C12Q1/6841

Abstract: The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein address one or more issues associated with the stability and/or size of nucleic acid structures such as rolling circle amplification products in the biological sample.

公开(公告)号：US20230013775A1

公开(公告)日：2023-01-19

申请号：US17812075

申请日：2022-07-12

Applicant: 10x Genomics, Inc.

Inventor： Hong CHEN ,  Evan R. DAUGHARTHY

IPC: C12Q1/6841 ,  G01N21/64 ,  G01N33/543

Abstract: The present disclosure relates in some aspects to methods for preparing biological samples for in situ analysis of one or more analytes, wherein the biological sample has been previously affixed to a substrate, which is not compatible with in situ analysis, for example, due to the absence of positional markers and/or fiducial markers and/or a region suitable for in situ signal detection on the substrate.