公开(公告)号：US12104173B2

公开(公告)日：2024-10-01

申请号：US16962973

申请日：2019-01-15

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Jaichandran Sivalingam ,  Steve Oh ,  Shaul Reuveny

IPC: C12N5/0789 ,  C12N5/078

CPC classification number: C12N5/0647 ,  C12N5/0641 ,  C12N2501/10 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/20 ,  C12N2501/392 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/00 ,  C12N2506/11 ,  C12N2527/00

Abstract: Methods of differentiation of pluripotent stem cells into hematopoietic precursor cells, wherein the method is carried out under suspension agitation, and wherein a GSK-3-inhibitor or a Wnt pathway activator is added during a stage of mesoderm induction, and cell culture media for use in the methods, as well as kits for performing the same.