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    Microcarriers for stem cell culture
    2.
    发明授权
    Microcarriers for stem cell culture 有权

    公开(公告)号：US09458431B2

    公开(公告)日：2016-10-04

    申请号：US13968214

    申请日：2013-10-29

    Applicant: Agency for Science, Technology and Research

    Inventor： Steve Oh ,  Shaul Reuveny ,  Jian Li ,  William Richard Nicholas Birch

    IPC: C12N5/00 ,  C12N5/02 ,  C12N5/074 ,  C12N5/0735 ,  C12M1/12

    CPC classification number: C12N5/0696 ,  C12M25/16 ,  C12N5/0606 ,  C12N2531/00 ,  C12N2533/10 ,  C12N2533/32 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2533/78 ,  C12N2533/80 ,  C12N2533/90

    Abstract: We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

    Microcarriers for stem cell culture
    3.
    发明授权
    Microcarriers for stem cell culture 有权
    用于干细胞培养的微载体

    公开(公告)号：US09340770B2

    公开(公告)日：2016-05-17

    申请号：US14134275

    申请日：2013-12-19

    Applicant: Agency for Science, Technology and Research

    Inventor： Steve Oh ,  Marti Lecina ,  Andre Choo ,  Shaul Reuveny ,  Robert Zweigerdt ,  Allen Chen

    IPC: C12N5/00 ,  C12N5/0735 ,  C12N5/0789

    CPC classification number: C12N5/0606 ,  C12N5/0062 ,  C12N5/0075 ,  C12N5/0647

    Abstract: We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

    Abstract translation: 我们公开了包含其上涂覆有基质并具有正电荷的颗粒的颗粒，该颗粒的尺寸允许附着于其上的灵长类动物或人类干细胞的聚集。 颗粒可以包括具有50μm至400μm之间的最长尺寸（例如约200μm）的基本细长的圆柱形或棒状颗粒。 它的横截面尺寸可以在20μm和30μm之间。 该颗粒可以包括具有约20μm和约120μm之间，例如约65μm的尺寸的基本致密或球形的颗粒。 我们还公开了增加灵长类动物或人类干细胞的方法，该方法包括：提供连接到第一和第二相应颗粒上的第一和第二灵长类动物或人类干细胞，使第一灵长类动物或人类干细胞接触第二灵长类动物或人类干细胞 细胞以形成细胞聚集体并培养所述聚集体以使所述灵长类动物或人类干细胞传播至少一个通道。 还公开了使用包被在基质胶或透明质酸中的微载体在长期悬浮培养物中繁殖人胚胎干细胞（hESC）的方法。 我们还公开了一种区分干细胞的方法。

    Microcarriers for Stem Cell Culture
    4.
    发明申请
    Microcarriers for Stem Cell Culture 有权

    公开(公告)号：US20140315300A1

    公开(公告)日：2014-10-23

    申请号：US13968214

    申请日：2013-10-29

    Applicant: Agency for Science, Technology and Research

    Inventor： Steve Oh ,  Shaul Reuveny ,  Jian Li ,  William Richard Nicholas Birch

    IPC: C12N5/074 ,  C12N5/0735

    CPC classification number: C12N5/0696 ,  C12M25/16 ,  C12N5/0606 ,  C12N2531/00 ,  C12N2533/10 ,  C12N2533/32 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2533/78 ,  C12N2533/80 ,  C12N2533/90

    Abstract: We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

    Microcarriers for Stem Cell Culture
    5.
    发明申请
    Microcarriers for Stem Cell Culture 有权
    干细胞培养的微载体

    公开(公告)号：US20140193903A1

    公开(公告)日：2014-07-10

    申请号：US14134275

    申请日：2013-12-19

    Applicant: Agency for Science, Technology and Research

    Inventor： Steve Oh ,  Marti Lecina ,  Andre Choo ,  Shaul Reuveny ,  Robert Zweigert ,  Allen Chen

    IPC: C12N5/0735

    CPC classification number: C12N5/0606 ,  C12N5/0062 ,  C12N5/0075 ,  C12N5/0647

    Abstract: We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

    Abstract translation: 我们公开了包含其上涂覆有基质并具有正电荷的颗粒的颗粒，该颗粒的尺寸允许附着于其上的灵长类动物或人类干细胞的聚集。 颗粒可以包括具有50μm至400μm之间的最长尺寸（例如约200μm）的基本细长的圆柱形或棒状颗粒。 它的横截面尺寸可以在20μm和30μm之间。 该颗粒可以包括具有约20μm和约120μm之间，例如约65μm的尺寸的基本致密或球形的颗粒。 我们还公开了增加灵长类动物或人类干细胞的方法，该方法包括：提供连接到第一和第二相应颗粒上的第一和第二灵长类动物或人类干细胞，使第一灵长类动物或人类干细胞接触第二灵长类动物或人类干细胞 细胞以形成细胞聚集体并培养所述聚集体以使所述灵长类动物或人类干细胞传播至少一个通道。 还公开了使用包被在基质胶或透明质酸中的微载体在长期悬浮培养物中繁殖人胚胎干细胞（hESC）的方法。 我们还公开了一种区分干细胞的方法。

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