公开(公告)号：US20240318142A1

公开(公告)日：2024-09-26

申请号：US18682870

申请日：2022-08-10

Applicant: KYOTO UNIVERSITY ,  REGE NEPHRO CO., LTD.

Inventor： Kenji OSAFUNE ,  Hiraku TSUJIMOTO

IPC: C12N5/071

CPC classification number: C12N5/0687 ,  C12N2500/38 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/2301 ,  C12N2501/41 ,  C12N2501/727 ,  C12N2506/45

Abstract: A method of producing renal interstitial progenitor cells, the method including the step of culturing HOXC10-positive cells in a medium comprising a GSK (glycogen synthase kinase)-3β inhibitor, a SHH (Sonic Hedgehog) signal activator, and a TGF (transforming growth factor) β inhibitor, and preferably also comprising IL (interleukin)-1β and retinoic acid, to induce the renal interstitial progenitor cells.

公开(公告)号：US20240318133A1

公开(公告)日：2024-09-26

申请号：US18731343

申请日：2024-06-02

Applicant: Upside Foods, Inc.

Inventor： Kalle Lukyan JOHNSON ,  Patricia R. BENTON ,  Cameron Gentry COPELAND ,  Sukhdeep Singh DHADWAR ,  Meri Teresa FIRPO ,  Marie Elizabeth GIBBONS ,  Stephen K. HSU ,  Robert SIERRA ,  Kevin John KAYSER

IPC: C12N5/071

CPC classification number: C12N5/0602 ,  C12N2500/14 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/33

Abstract: Provided herein are nutrient media formulations and engineered growth factors, and methods thereof, useful for the production of slaughter-free meat.

公开(公告)号：US20240309334A1

公开(公告)日：2024-09-19

申请号：US18121340

申请日：2023-03-14

Applicant: ReGen Theranostics, Inc.

Inventor： Timothy Nelson

IPC: C12N5/074 ,  A01N1/02 ,  C12N15/86

CPC classification number: C12N5/0696 ,  A01N1/0284 ,  C12N15/86 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/60 ,  C12N2506/1307 ,  C12N2740/15043

Abstract: In some embodiments, the present disclosure is directed to methods of reprogramming fibroblasts into induced pluripotent stem cells. In some embodiments, a method comprises obtaining fibroblasts, such as from a skin biopsy. In some embodiments, a method comprises reprogramming fibroblasts using a viral vector encoding reprogramming factors. In some embodiments, a method comprises expanding iPSC and identifying confluent iPSC.

公开(公告)号：US20240299462A1

公开(公告)日：2024-09-12

申请号：US18662902

申请日：2024-05-13

Applicant: ViaCyte, Inc.

Inventor： Alan D. Agulnick ,  Kevin Allen D'Amour

IPC: A61K35/39 ,  A61K35/545 ,  C12N5/071

CPC classification number: A61K35/39 ,  A61K35/545 ,  C12N5/0676 ,  C12N5/0678 ,  C12N2500/25 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/195 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2533/90

Abstract: A human immature endocrine cell population and methods for making an immature endocrine cell population are provided. Specifically, immature beta cells and methods for production of immature beta cells are described. Immature beta cells co-express INS and NKX6.1 and are uni-potent and thereby develop into mature beta cells when implanted in vivo. The mature beta cells in vivo are capable of producing insulin in response to glucose stimulation.

公开(公告)号：US12071648B2

公开(公告)日：2024-08-27

申请号：US16650492

申请日：2018-09-25

Applicant: GENOSKIN

Inventor： Claire Jardet ,  Pascal Descargues ,  Hanne Norsgaard ,  Paola Lovato

IPC: C12Q1/02 ,  C12N5/071

CPC classification number: C12Q1/025 ,  C12N5/0629 ,  C12N2501/15 ,  C12N2501/2301 ,  C12N2501/2302 ,  C12N2501/2323 ,  C12N2501/51 ,  C12N2501/515

Abstract: Disclosed is an ex-vivo model of inflamed skin, an in-vitro method for obtaining the same and uses thereof for screening anti-inflammatory compounds. The method includes injecting, into the dermis of a healthy skin biopsy previously taken from a mammal, a composition including an effective amount for activating dermal resident T cells of an anti-CD3 antibody and an anti-CD28 antibody; and b) incubating the injected skin biopsy obtained in step a) in the presence of a composition including an effective amount, for obtaining the polarization of the T cells activated in step a) into LTh1 and/or LTh17 and the synthesis of inflammation markers, of at least one mixture of IL-1β, IL-23, and TGF-β.

公开(公告)号：US20240279612A1

公开(公告)日：2024-08-22

申请号：US18553316

申请日：2022-03-31

Applicant: INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE ,  NANTES UNIVERSITÉ ,  HCS PHARMA ,  CNRS (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE) ,  CHU DE NANTES

Inventor： Karim SI-TAYEB ,  Amandine CAILLAUD ,  Nathalie MAUBON ,  Zied SOUGUIR ,  Meryl ROUDAUT

IPC: C12N5/071

CPC classification number: C12N5/0671 ,  C12N2500/02 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/2306 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/45 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2537/10

Abstract: The present disclosure relates to methods for preparing a liver organoid, as well as liver organoids obtained with the same and uses thereof. It was observed that, in presence of a set of suitable cell culture media, a use of a 3D porous scaffold and of alternated steps of hypoxic and normoxic conditions was able to drive the differentiation of stems cells in functional liver organoids comprising at least in hepatocytes, cholangiocytes, stellate cells, and sinusoidal endothelial cells. The obtained liver organoids expressed liver-specific genes, responded to LDL internalization, showed lipid accumulation and presented a strong activity of CYP enzymes.

公开(公告)号：US12065674B2

公开(公告)日：2024-08-20

申请号：US17856085

申请日：2022-07-01

Applicant: YALE UNIVERSITY

Inventor： Mahboobe Ghaedi ,  Laura Niklason

IPC: C12N5/071 ,  A61K35/36

CPC classification number: C12N5/0688 ,  A61K35/36 ,  C12N5/0689 ,  C12N2500/99 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/415 ,  C12N2506/45

Abstract: The present invention provides compositions and methods of preparing airway cells. In one aspect, an epithelial airway cell derived from an induced pluripotent stem (iPS) cell characterized by expression of airway cell surface markers and an ability to proliferate is described. In another aspect, methods of differentiating an iPS into an epithelial airway cell is provided. Engineered lungs, methods of making such engineered lungs comprising the epithelial airway cells and treating respiratory disorders are also disclosed.

公开(公告)号：US12065671B2

公开(公告)日：2024-08-20

申请号：US16931003

申请日：2020-07-16

Applicant: The United States of America, as represented by the Secretary, Department of Health and Human Services ,  FUJIFILM Cellular Dynamics, Inc.

Inventor： Kapil Bharti ,  Lucas Chase ,  Feng Xuezhu ,  Balendu Shekhar Jha

IPC: C12N5/079

CPC classification number: C12N5/0621 ,  C12N2500/30 ,  C12N2501/105 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/415 ,  C12N2506/45 ,  C12N2533/50 ,  C12N2533/52

Abstract: Provided herein are methods of producing an RPE cell population from a starting cell suspension, such as a single cell suspension, of pluripotent stem cells (PSCs). Such a method may comprise culturing the starting single cell suspension of PSCs in differentiation media to produce human RPE cells.

公开(公告)号：US12060575B2

公开(公告)日：2024-08-13

申请号：US17178279

申请日：2021-02-18

Applicant: Technion Research & Development Foundation Limited

Inventor： Michal Amit ,  Joseph Itskovitz-Eldor

IPC: C12N5/0735 ,  C12N5/00

CPC classification number: C12N5/0606 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0043 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/2306 ,  C12N2501/235 ,  C12N2533/12 ,  C12N2533/90

Abstract: A method of expanding and maintaining human embryonic stem cells (ESCs) in an undifferentiated state by culturing the ESCs in a suspension culture under culturing conditions devoid of substrate adherence is provided. Also provided are a method of deriving ESC lines in the suspension culture and methods of generating lineage-specific cells from ESCs which were expanded in the suspension culture of the present invention.

公开(公告)号：US20240264152A1

公开(公告)日：2024-08-08

申请号：US18378530

申请日：2023-10-10

Applicant: The Regents of the University of California

Inventor： Alysson Renato Muotri ,  Priscilla Davidson Negraes ,  Cleber Trujillo ,  Pinar Mesci

IPC: G01N33/50 ,  C12N5/0789

CPC classification number: G01N33/5058 ,  C12N5/0647 ,  G01N33/5011 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155

Abstract: The disclosure provides methods of making functional cortical organoids from somatic cells and stem cells and methods of using the functional organoids.